Heterologous expression and secretion of Lactobacillus amylovorus α-amylase in Leuconostoc citreum

Biotechnology Letters

Volumn 31, Issue 11, 2009, Pages 1783-1788

  Eom, Hyun Ju ^a   Moon, Jin Seok ^a   Seo, Eun Young ^a   Han, Nam Soo ^a  

^a Chungbuk National University   (South Korea)

Author keywords

Amylase; Heterologous protein; Leuconostoc citreum; PLeuCM; Segregational stability

Indexed keywords

AMYLASE ACTIVITY; AMYLASE GENES; CLONING VECTORS; CULTURE BROTHS; EXPRESSION VECTORS; HETEROLOGOUS EXPRESSION; HETEROLOGOUS PROTEIN; HETEROLOGOUS PROTEINS; HOST-VECTOR SYSTEM; LEUCONOSTOC; LEUCONOSTOC CITREUM; PLEUCM; SEGREGATIONAL STABILITY; SIGNAL PEPTIDE; SIGNAL SEQUENCE;

AMINES; CELL CULTURE; CLONING; ENZYME ACTIVITY; GENE EXPRESSION;

SYSTEM STABILITY;

AMYLASE;

ARTICLE; BACTERIAL GENE; CYTOLOGY; DNA SEQUENCE; ENZYMOLOGY; GENETIC TRANSFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; LACTOBACILLUS ACIDOPHILUS; LEUCONOSTOC; METABOLISM; MOLECULAR CLONING; PLASMID; RESTRICTION MAPPING; SECRETION;

ALPHA-AMYLASES; CLONING, MOLECULAR; GENES, BACTERIAL; LACTOBACILLUS ACIDOPHILUS; LEUCONOSTOC; PLASMIDS; RESTRICTION MAPPING; SEQUENCE ANALYSIS, DNA; TRANSFORMATION, GENETIC;

LACTOBACILLUS AMYLOVORUS; LEUCONOSTOC; LEUCONOSTOC CITREUM;

EID: 70350294290     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-0079-1     Document Type: Article

References (17)

1. Biet F, Berjeaud JM, Worobo RW, Cenatiempo Y, Fremaux C (1998) Heterologous expression of the bacteriocin mesentericin Y105 using the decicated transport system and the general secretion pathway. Microbiology 144:2845-2854.
2. Biet F, Cenatiempo Y, Fremoux C (1999) Characterization of pFR18, a small cryptic plasmid from Leuconostoc mesenteroides ssp. mesenteroides FR52, and its use as a food-grade vector. FEMS Microbiol Lett 179:375-383.
3. Chang H, Choi Y, Lee H (1996) Molecular cloning of a β-d-galactosidase gene from Lactococcus lactis subsp. lactis. J Microbiol Biotechnol 6:386-390.
4. Eom HJ, Park JM, Seo MJ, Kim MD, Han NS (2008) Monitoring of Leuconostoc mesenteroides DRC starter in fermented vegetable by random integration of chloramphenicol acetyltransferase gene. J Ind Microbiol Biotechnol 35:953-959.
5. Giraud E, Cuny G (1997) Molecular characterization of the a-amylase genes of Lactobacillus plantarum A6 and Lactobacillus amylovorus reveals an unusual 3′ end structure with direct tandem repeats and suggests a common evolutionary origin. Gene 198:149-157.
6. Hemme D, Catherine FS (2004) Leuconostoc, characteristics, use in dairy technology and prospects in functional foods. Int Dairy J14:467-494.
7. Hols P, Ferain T, Garmyn D, Bernard N, Delcour J (1994) Use of homologous expression-secretion signals and vector-fress stable chromosomal integration in engineering of Lactobacillus plantarum for α-amylase and levanase expression. Appl Environ Microbiol 60:1401-1413.
8. Jeong SJ, Park JY, Kim JH et al (2006) Transformation of Leuconostoc mesenteroides SY1, a strain isolated from kimchi. J Microbiol Biotechnol 16:149-152.
9. Kiewiet R, Bron S, de Jonge K, Venema G, Seegers JF (1993) Theta replication of the lactococcal plasmid pWV02. Mol Microbiol 10:319-327.
10. Kim JF, Jeong H, Lee JS et al (2008) Complete genome sequence of Leuconostoc citreum KM20. J Bacteriol 190:3093-3094.
11. Lantz MS, Ciborowski P (1994) Zymographic techniques for detection and characterization of microbial proteases. Methods Enzymol 235:563-594.
12. Miller AW, Eklund SH, Robyt JF (1986) Milligram to gram scale purification and characterization of dextransucrase from Leuconostoc mesenteroides NRRL B-512F. Carbohydr Res 147:119-133.
13. Neubauer H, Bauché A, Mollet B (2003) Molecular characterization and expression analysis of the dextransucrase DsrD of Leuconostoc mesenteroides Lcc4 in homologous and heterologous Lactococcus lactis cultures. Microbiology 149:973-982.
14. Rhim SL, Park MS, Ji GE (2006) Expression and secretion of Bifidobacterium adolescentis amylase by Bifodobacterium longum. Biotechnol Lett 28:163-168.
15. Sambrook J, Fritsch EF, Maniatis T (2001) Molecular cloning: A laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
16. Savijoki K, Kahala M, Palva A (1997) High level heterologous protein production in Lactococcus and Lactobacillus using a new secretion system based on the Lactobacillus brevis S-layer signals. Gene 86:255-262.
17. Shareck J, Choi Y, Lee B, Miguez CB (2004) Cloning vectors based on cryptic plasmids isolated from lactic acid bacteria: Their characteristics and potential applications in biotechnology. Crit Rev Biotechnol 24:155-208.