Cloning and overexpression of a thermostable signal peptide peptidase (SppA) from Thermoplasma volcanium GSS1 in E. coli

Biotechnology Journal

Volumn 4, Issue 7, 2009, Pages 1055-1065

  Kocabiyik, Semra ^a   Demirok, Burçak ^a  

^a MIDDLE EAST TECHNICAL UNIVERSITY   (Turkey)

Author keywords

Serine protease; Signal peptide peptidase; Thermophilic; Thermoplasma volcanium

Indexed keywords

ANION-EXCHANGE COLUMN CHROMATOGRAPHY; ARCHAEA; ARCHAEON; CHYMOSTATIN; DOMAIN ANALYSIS; E. COLI; ENZYME ASSAYS; GENE CODING; GENERAL BASE; HIGH TEMPERATURE; MULTIPLE SEQUENCE ALIGNMENTS; OVER-EXPRESSION; PRIMARY SEQUENCES; RECOMBINANT ENZYMES; SDS-PAGE ANALYSIS; SERINE PROTEASE; SIGNAL PEPTIDE; SIGNAL PEPTIDE PEPTIDASE; THERMOPHILIC; THERMOPLASMA VOLCANIUM; TRANS-MEMBRANE DOMAINS;

ACTIVATION ANALYSIS; AMINES; AMINO ACIDS; CALCIUM; CHROMATOGRAPHIC ANALYSIS; CLONING; COLUMN CHROMATOGRAPHY; DIFFUSERS (OPTICAL); ENZYME ACTIVITY; ENZYMES; ESCHERICHIA COLI; PEPTIDES; PURIFICATION;

GENE ENCODING;

CALCIUM ION; LYSINE; RECOMBINANT ENZYME; SERINE; SIGNAL PEPTIDASE;

AMINO ACID SEQUENCE; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ESCHERICHIA COLI; GENE OVEREXPRESSION; HIGH TEMPERATURE; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; THERMOPLASMA; THERMOPLASMA VOLCANIUM;

AMINO ACID SEQUENCE; ARCHAEAL PROTEINS; ASPARTIC ENDOPEPTIDASES; BASE SEQUENCE; CALCIUM; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; EDETIC ACID; ENZYME STABILITY; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; PHYLOGENY; PROTEASE INHIBITORS; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; THERMOPLASMA;

ARCHAEA; ESCHERICHIA COLI; THERMOPLASMA VOLCANIUM;

EID: 70350227206     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.200800344     Document Type: Article

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