Production of 5,8,11-eicosatrienoic acid (Mead acid) by a Δ6 desaturation activity-enhanced mutant derived from a Δ12 desaturase- defective mutant of an arachidonic acid-producing fungus, Mortierella alpina 1S-4

Applied and Environmental Microbiology

Volumn 63, Issue 5, 1997, Pages 1820-1825

  Kawashima, Hiroshi ^a   Nishihara, Mitsuhiro ^b   Hirano, Yuriko ^b   Kamada, Nozomu ^b   Akimoto, Kengo ^a   Konishi, Kyoko ^a   Shimizu, Sakayu ^b  

^a SUNTORY LTD   (Japan)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

5,8,11 ICOSATRIENOIC ACID; ARACHIDONIC ACID; FATTY ACID; TRIACYLGLYCEROL;

ARTICLE; CULTURE MEDIUM; ENZYME ACTIVITY; ESSENTIAL FATTY ACID DEFICIENCY; FATTY ACID METABOLISM; FERMENTATION; FUNGUS MUTANT; LIPID COMPOSITION; MYCELIUM; NONHUMAN; TEMPERATURE;

EID: 0030894937     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.63.5.1820-1825.1997     Document Type: Article

References (24)

1. Adkisson, A. D., F. S. Risener, Jr., P. P. Zarrinkar, M. D. Walla, W. W. Christie, and R. E. Wuthier. 1991. Unique fatty acid composition of normal cartilage: discovery of high levels of n-9 eicosatrienoic acid and low levels of n-6 polyunsaturated fatty acids. FASEB J. 5:344-353.
2. Brenner, R. R., and R. O. Peluffo. 1966. Effect of saturated and unsaturated fatty acids on the desaturation in vitro of palmitic, stearic, oleic, linoleic, and linolenic acids. J. Biol. Chem. 241:5213-5219.
3. Cleland, K. A., M. J. James, M. A. Neumann, R. A. Gibson, and L. G. Cleland. 1995. Differences in fatty acid composition of immature and mature articular cartilage in humans and sheep. Lipids 30:949-953.
4. 4 synthesis in essential fatty acid deficiency: role of leukotriene A hydrolase. Lipids 29: 151-155.
5. Folch, J., M. Lees, and G. H. Sloane-Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497-509.
6. Fulco, A. J., and J. F. Mead. 1959. Metabolism of essential fatty acids. J. Biol. Chem. 234:1411-1416.
7. Ghosh, A., M. Koley, and J. Dutta. 1982. Preparation of cis,cis,cis-5,8,11-eicosatrienoic acid from arachidonic acid. Lipids 17:314-316.
8. 3. J. Biol. Chem. 256:2275-2279.
9. Hornsta, G., A. C. Houwelinggen, M. Simonis, and J. M. Gerrand. 1989. Fatty acid composition of umbilical arteries and veins: possible implications for the fetal EFA-status. Lipids 24:511-517.
10. 4 synthesis in rats: a novel approach to inhibition of eicosanoid synthesis. J. Exp. Med. 178:2261-2265.
11. Jareonkitmongkol, S., H. Kawashima, S. Shimizu, and H. Yamada. 1992. Production of 5,8,11-cis-eicosatrienoic acid by a Δ12-desaturase-defective mutant of Mortierella alpina 1S-4. J. Am. Oil Chem. Soc. 69:939-944.
12. Jareonkitmongkol, S., H. Kawashima, N. Shirasaka, S. Shimizu, and H. Yamada. 1992. Production of dihomo-γ-linolenic acid by a Δ5-desaturase-defective mutant of Mortierella alpina 1S-4. Appl. Environ. Microbiol. 58: 2196-2200.
13. Jareonkitmongkol, S., E. Sakuradani, and S. Shimizu. 1993. A novel Δ5-desaturase-defective mutant of Mortierella alpina 1S-4 and its dihomo-γ-linolenic acid productivity. Appl. Environ. Microbiol. 59:4300-4304.
14. Jareonkitmongkol, S., S. Shimizu, and H. Yamada. 1992. Fatty acid desaturation-defective mutants of an arachidonic-acid-producing fungus, Mortierella alpina 1S-4. J. Gen. Microbiol. 138:997-1002.
15. 6-desaturase-defective mutant of the fungus Mortierella alpina 1S-4. Biochim. Biophys. Acta 1167:137-141.
16. Jareonkitmongkol, S., S. Shimizu, and H. Yamada. 1993. Production of an eicosapentaenoic acid-containing oil by a Δ12 desaturase-defective mutant of Mortierella alpina 1S-4. J. Am. Oil Chem. Soc. 70:119-123.
17. Kawashima, H., K. Akimoto, N. Shirasaka, and S. Shimizu. 1996. Inhibitory effect of alkyl gallate and its derivatives on fatty acid desaturation. Biochim. Biophys. Acta 1299:34-38.
18. 2-like activity of 20:3n-9 platelet lipoxygenase end-product. FEBS Lett. 181:53-56.
19. Mead, J. F., and W. H. Slaton, Jr. 1956. Metabolism of essential fatty acids. III. Isolation of 5,8,11-eicosatrienoic acid from fat-deficient rats. J. Biol. Chem. 219:705-709.
20. Shimizu, S., S. Jareonkitmongkol, H. Kawashima, K. Akimoto, and H. Yamada. 1991. Production of a novel ω1-eicosapentaenoic acid by Mortierella alpina 1S-4 grown on 1-hexadecene. Arch. Microbiol. 156:163-166.
21. Shimizu, S., H. Kawashima, K. Akimoto, Y. Shinmen, and H. Yamada. 1989. Conversion of linseed oil to an eicosapentaenoic acid-containing oil by Mortierella alpina 1S-4 at low temperature. Appl. Microbiol. Biotechnol. 32:1-4.
22. Shinmen, Y., S. Shimizu, K. Akimoto, H. Kawashima, and H. Yamada. 1989. Production of arachidonic acid by Mortierella fungi: selection of a potent producer and optimization of culture conditions for large-scale production. Appl. Microbiol. Biotechnol. 31:11-16.
23. Werner, W., H.-G. Rey, and H. Wielinger. 1970. Über die Eigenschaften eines neuen Chromogens für die Blutzuckerbestimmung nach der GOD/ POD-Methode. Z. Anal. Chem. 252:224-228.
24. Yamada, H., S. Shimizu, and Y. Shinmen. 1987. Production of arachidonic acid by Mortierella elongata 1S-5. Agric. Biol. Chem. 51:785-790.