High-level expression and large-scale preparation of soluble HBx antigen from Escherichia coli

Biotechnology and Applied Biochemistry

Volumn 54, Issue 3, 2009, Pages 141-147

  Liu, Dong ^a   Zou, Liyun ^a   Li, Wanling ^a   Wang, Li ^a   Wu, Yuzhang ^a  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Amylose resin chromatography; Escherichia coli strain JM109; Hepatitis B virus X protein (HBx); Hepatocellular carcinoma; Q Sepharose chromatography; Sephadex G 75 chromatography

Indexed keywords

AMYLOSE RESIN CHROMATOGRAPHY; HEPATITIS B VIRUS X PROTEIN (HBX); HEPATOCELLULAR CARCINOMA; Q-SEPHAROSE CHROMATOGRAPHY; SEPHADEX G-75 CHROMATOGRAPHY;

CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; DEXTRAN; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; ION EXCHANGE; PURIFICATION; RESINS; SIGNAL TRANSDUCTION;

CRYSTAL STRUCTURE;

AMYLOSE; HEPATITIS B VIRUS X PROTEIN; HYBRID PROTEIN; SEPHAROSE;

ALPHA HELIX; ANTIGEN EXPRESSION; ARTICLE; BETA SHEET; CHROMATOGRAPHY; CONTROLLED STUDY; CRYSTAL STRUCTURE; ESCHERICHIA COLI; IMMUNOGENICITY; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN SECONDARY STRUCTURE;

CARRIER PROTEINS; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, ION EXCHANGE; CIRCULAR DICHROISM; ESCHERICHIA COLI; FACTOR XA; RECOMBINANT FUSION PROTEINS; TRANS-ACTIVATORS;

ESCHERICHIA COLI; HEPATITIS B VIRUS;

EID: 70350169727     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20090116     Document Type: Article

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