Gene-specific silencing induced by parallel complementary RNA in Pseudomonas aeruginosa

Biotechnology Letters

Volumn 31, Issue 10, 2009, Pages 1571-1575

  Liu, Yi Shuang ^a   Zhang, Yue Qin ^a   Yang, Li Xia ^a   Yao, Tian Jue ^a   Xiao, Chun Ling ^a  

^a INSTITUTE OF MEDICINAL BIOTECHNOLOGY   (China)

Author keywords

Efflux pump; Gene specific silencing; mexA; Parallel complementary RNA; Pseudomonas aeruginosa

Indexed keywords

ACCUMULATION RATES; ANTI-MICROBIAL AGENT; CONTROL STRAIN; EFFLUX PUMP; EFFLUX PUMPS; ETHIDIUM BROMIDE; GENE REGULATIONS; GENE SILENCING; MEXA; MINIMUM INHIBITORY CONCENTRATION; PARALLEL COMPLEMENTARY RNA; PSEUDOMONAS AERUGINOSA;

ESCHERICHIA COLI; GENETIC ENGINEERING; RNA; STRAIN;

NUCLEIC ACIDS;

ANTIINFECTIVE AGENT; CARRIER PROTEIN; COMPLEMENTARY RNA; MEXA PROTEIN, PSEUDOMONAS AERUGINOSA; OUTER MEMBRANE PROTEIN;

ARTICLE; DRUG ANTAGONISM; GENE SILENCING; GENETICS; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; PSEUDOMONAS AERUGINOSA;

ANTI-BACTERIAL AGENTS; BACTERIAL OUTER MEMBRANE PROTEINS; GENE KNOCKDOWN TECHNIQUES; GENE SILENCING; MEMBRANE TRANSPORT PROTEINS; MICROBIAL SENSITIVITY TESTS; PSEUDOMONAS AERUGINOSA; RNA, COMPLEMENTARY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PROKARYOTA; PSEUDOMONAS AERUGINOSA;

EID: 70350103536     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-0046-x     Document Type: Article

References (14)

1. Akama H, Matsuura T, Kashiwagi S et al (2004) Crystal structure of the membrane fusion protein, MexA, of the multidrug transporter in Pseudomonas aeruginosa. J Biol Chem 279:25939-25942.
2. Devienne K, Raddi M (2002) Screening for antimicrobial activity of natural products using a microplate photometer. Braz J Microbiol 33:166-168.
3. Fire A, Xu S, Montgomery MK et al (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391:806-811.
4. Makarova KS, Grishin NV, Shabalina SA et al (2006) A putative RNA-interference-based immune system in prokaryotes: computational analysis of the predicted enzymatic machinery, functional analogies with eukaryotic RNAi, and hypothetical mechanisms of action. Biol Direct 1:7.
5. Masuda N, Sakagawa E, Ohya S et al (2000) Substrate specificities of MexAB-OprM, MexCD-OprJ, and MexXY-oprM efflux pumps in Pseudomonas aeruginosa. Antimicrob Agents Chemother 44:3322-3327.
6. Narita S, Eda S, Yoshihara E et al (2003) Linkage of the efflux-pump expression level with substrate extrusion rate in the MexAB-OprM efflux pump of Pseudomonas aeruginosa. Biochem Biophys Res Commun 308:922-926.
7. Nehme D, Li XZ, Elliot R et al (2004) Assembly of the MexAB-OprM multidrug efflux system of Pseudomonas aeruginosa: identification and characterization of mutations in mexA compromising MexA multimerization and interaction with MexB. J Bacteriol 186:2973-2983.
8. Ocaktan A, Yoneyama H, Nakae T (1997) Use of fluorescence probes to monitor function of the subunit proteins of the MexA-MexB-OprM drug extrusion machinery in Pseudomonas aeruginosa. J Biol Chem 272:21964-21969.
9. Ponomarenko NA, Chistiakova LG, Chernov BK et al (1992) Formation of a parallel RNA-RNA duplex in vivo in Escherichia coli cells. Dokl Akad Nauk 327:584-588.
10. Poole K, Tetro K, Zhao Q et al (1996) Expression of the multidrug resistance operon mexA-mexB-oprM in Pseudomonas aeruginosa: mexR encodes a regulator of operon expression. Antimicrob Agents Chemother 40:2021-2028.
11. Tchurikov NA (1992) Natural DNA sequences complementary in the same direction: evidence for parallel biosynthesis? Genetica 87:113-117.
12. Tchurikov NA, Ponomarenko NA, Golova YB et al (1995) The formation of parallel RNA-RNA duplexes in vitro. J Biomol Struct Dyn 13:507-513.
13. Tchurikov NA, Chistyakova LG, Zavilgelsky GB et al (2000) Gene-specific silencing by expression of parallel complementary RNA in Escherichia coli. J Biol Chem 275:26523-26529.
14. Win MN, Smolke CD (2007) RNA as a versatile and powerful platform for engineering genetic regulatory tools. Biotechnol Genet Eng Rev 24:311-346.