Leishmania infantum: Antiproliferative effect of recombinant plant cystatins on promastigotes and intracellular amastigotes estimated by direct counting and real-time PCR

Experimental Parasitology

Volumn 123, Issue 4, 2009, Pages 341-346

  Ordonez Gutierrez L ^a   Martinez M ^b   Rubio Somoza, I ^b   Diaz I ^b   Mendez, S ^c   Alunda, J M ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b ETSI MontesTechnical University of Madrid   (Spain)

^c Cornell University ^*  (United States)

Author keywords

Amastigotes; Cystatins; Cysteine proteases; Leishmania; Macrophages; Promastigotes; Q PCR

Indexed keywords

CYSTATIN; CYSTEINE PROTEINASE;

AMASTIGOTE; ANIMAL CELL; ARTICLE; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; DRUG MECHANISM; LEISHMANIA INFANTUM; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMASTIGOTE; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION;

ANIMALS; CELL LINE; CELL SURVIVAL; CYSTATINS; CYSTEINE PROTEINASE INHIBITORS; DNA, PROTOZOAN; DOGS; DOSE-RESPONSE RELATIONSHIP, DRUG; HORDEUM; LEISHMANIA INFANTUM; MACROPHAGES; POLYMERASE CHAIN REACTION;

HORDEUM; LEISHMANIA INFANTUM; MAMMALIA;

EID: 70350031599     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2009.08.015     Document Type: Article

References (36)

1. Abraham Z., Martínez M., Carbonero P., and Díaz I. Structural and functional diversity within the cystatin gene family of Hordeum vulgare. Journal of Experimental Botany 57 (2006) 4245-4255
2. Buxbaum L.U., Denise H., Coombs G.H., Alexander J., Mottram J.C., and Scott P. Cysteine protease B of Leishmania mexicana inhibits host Th1 responses and protective immunity. The Journal of Immunology 171 (2003) 3711-3717
3. Croft S.L., and Coombs G.H. Leishmaniasis-current chemotherapy and recent advances in the search for novel drugs. Trends in Parasitology 19 (2003) 502-508
4. Croft S.L., Sundar S., and Fairlamb A.H. Drug resistance in leishmaniasis. Clinical Microbiology Reviews 19 (2006) 111-126
5. Chappuis F., Sundar S., Hailu A., Ghalib H., Rijal S., Peeling R.W., Alvar J., and Boelaert M. Visceral leishmaniasis: what are the needs for diagnosis, treatment and control?. Nature Reviews Microbiology 5 (2007) 873-882
6. Das L., Datta N., Bandyopadhyay S., and Das P.K. Successful therapy of lethal murine visceral leishmaniasis with cystatin involves up-regulation of nitric oxide and a favorable T cell response. Journal of Immunology 166 (2001) 4020-4028
7. Denise H., McNeil K., Brooks D.R., Alexander J., Coombs G.H., and Mottram J.C. Expression of multiple CPB genes encoding cysteine proteases is required for Leishmania mexicana virulence in vivo. Infection and Immunity 71 (2003) 3190-3195
8. Desjeux P. Leishmaniasis: current situation and new perspectives. Comparative Immunology Microbiology Infectious Diseases 27 (2004) 305-318
9. Doyle P.S., Zhou Y.M., Engel J.C., and Mckerrow J.H. A cysteine protease inhibitor cures Chagas' disease in an immunodeficient-mouse model of infection. Antimicrobial Agents and Chemotherapy 51 (2007) 3932-3939
10. Frame M.J., Mottram J.C., and Coombs G.H. Analysis of the roles of cysteine proteinases of Leishmania mexicana in the host-parasite interaction. Parasitology 121 (2000) 367-377
11. Gramiccia M., Gradoni L., and Orsini S. Decreased sensitivity to meglumine antimoniate (Glucantime) of Leishmania infantum isolated from dogs after several courses of drug treatment. Annals of Tropical Medicine and Parasitology 86 (1992) 613-620
12. Kominami E., Tsukahara T., Bando Y., and Katunuma N. Distribution of cathepsins B and H in rat tissues and peripheral blood cells. Journal of Biochemistry 98 (1985) 87-93
13. Kopitar-Jerala N. The role of cystatins in cells of the immune system. FEBS Letters 580 (2006) 6295-6301
14. Mahmoudzadeh-Niknam H., and Mckerrow J.H. Leishmania tropica: cysteine proteases are essential for growth and pathogenicity. Experimental Parasitology 106 (2004) 158-163
15. Martínez M., López-Solanilla E., Rodríguez-Palenzuela P., Carbonero P., and Díaz I. Inhibition of plant-pathogenic fungi by the barley cystatin Hv-CPI (gene Icy) is not associated with its cysteine-proteinase inhibitory properties. Molecular Plant-Microbe Interactions 16 (2003) 876-883
16. McKerrow J.H., Engel J.C., and Caffrey C.R. Cysteine protease inhibitors as chemotherapy for parasitic infections. Bioorganic and Medicina Chemistry 7 (1999) 639-644
17. McKerrow J.H., Caffrey C., Kelly B., Loke P., and Sajid M. Proteases in parasitic diseases. Annual Reviews of Pathology 1 (2006) 497-536
18. Méndez S., Nell M., and Alunda J.M. Leishmania infantum: infection of macrophages in vitro with promastigotes. International Journal for Parasitology 26 (1996) 619-622
19. Morillas F., Sánchez Rabasco F., Ocaña J., Martín-Sánchez J., Ocaña-Wihelmi J., Acedo C., and Sanchiz-Marín M.C. Leishmaniosis in the focus of the Axarquia region, Malaga province, southern Spain: a survey of the human, dog, and vector. Parasitology Research 82 (1996) 569-570
20. Mottram J.C., Brooks D.R., and Coombs G.H. Roles of cysteine proteinases of trypanosomes and Leishmania in host-parasite interactions. Current Opinion in Microbiology 1 (1998) 455-460
21. Mottram, J.C., Souza, A.E., Hutchison, J.E., Carter, R., Frame, M.J., Coombs, G.H. 1996. Proceedings of the National Academy of Sciences USA 93, 6008-6013.
22. Mukherjee S., Ukil A., and Das P.K. Immunomodulatory peptide from cystatin, a natural cysteine protease inhibitor, against leishmaniasis as a model macrophage disease. Antimicrobial Agents and Chemotherapy 51 (2007) 1700-1707
23. Mundodi, V., Kucknoor, A.S., Gedamu, L., 2005. Role of Leishmania (Leishmania) chagasi amastigote cysteine protease in intracellular parasite survival: studies by gene disruption and antisense mRNA inhibition. BMC Molecular Biology 6, 3. Available from: .
24. Ordóñez-Gutiérrez L., Espada-Fernández R., Dea-Ayuela M.A., Torrado J.J., Bolás-Fernández F., and Alunda J.M. In vitro effect of new formulations of amphotericin B on amastigote and promastigote forms of Leishmania infantum. International Journal of Antimicrobial Agents 30 (2007) 325-329
25. Rzychon M., Chmiel D., and Stec-Niemczyk J. Modes of inhibition of cysteine proteases. Acta Biochimica Polonica 51 (2004) 861-873
26. Sajid M., and McKerrow J.H. Cysteine proteases of parasitic organisms. Molecular Biochemical Parasitology 120 (2002) 1-21
27. Salvati L., Mattu M., Colasanti M., Scalone A., Venturini G., Gradoni L., and Ascenzi P. NO donors inhibit Leishmania infantum cysteine proteinase activity. Biochimica et Biophysica Acta 1545 (2001) 357-366
28. Saravia N.G., Escorcia B., Osorio Y., Valderrama L., Brooks D., Arteaga L., Coombs G., Mottram J., and Travi B.L. Pathogenicity and protective immunogenicity of cysteine proteinase-deficient mutants of Leishmania mexicana in non-murine models. Vaccine 24 (2006) 4247-4259
29. Selzer P.M., Pingel S., Hsieh I., Ugele B., Chan V.J., Engel J.C., Bogyo M., Russell D.G., Sakanari J.A., and Mckerrow J.H. Cysteine protease inhibitors as chemotherapy: lessons from a parasite target. Proceedings National Academy of Sciences of the United States of America 96 (1999) 11015-11022
30. Sereno D., Cordeiro da Silva A., Mathieu-Daude F., and Ouaissi A. Advances and perspectives in Leishmania cell based drug-screening procedures. Parasitology International 56 (2007) 3-7
31. Sundar S. Paromomycin in the treatment of leishmaniasis. Expert Opinion on Investigational Drugs 17 (2008) 787-794
32. Sundar S., and Chatterjee M. Visceral leishmaniasis-current therapeutic modalities. Indian Journal of Medical Research 123 (2006) 345-352
33. Turk B. Targeting proteases: successes, failures and future prospects. Nature Reviews 5 (2006) 785-799
34. Verdot L., Lalmanach G., Vercruysse V., Hartmann S., Lucius R., Hoebeke J., Gauthier F., and Vray B. Cystatins up-regulate nitric oxide release from Interferon-γ-activated mouse peritoneal macrophages. The Journal of Biological Chemistry 271 (1996) 28077-28081
35. Vray B., Hartmann S., and Hoebeke J. Immunomodulatory properties of cystatins. Celular and Molecular Life Sciences 59 (2002) 1503-1512
36. World Health Organization, WHO, 2000. Leishmaniasis control home page .