Biocompatible ionic liquids: A new approach for stabilizing proteins in liquid formulation

Journal of Biomechanical Engineering

Volumn 131, Issue 7, 2009, Pages

  Vrikkis, Regina M ^a   Fraser, Kevin J ^b   Fujita, Kyoko ^c   MacFarlane, Douglas R ^b   Elliott, Gloria D ^a  

^a University of North Carolina at Charlotte   (United States)

^b MONASH UNIVERSITY   (Australia)

^c TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

Author keywords

Biocompatibility; Ionic liquids; Protein stability

Indexed keywords

AQUEOUS BUFFER; DIHYDROGEN PHOSPHATE; DRUG FORMULATIONS; FUNCTIONAL ACTIVITIES; IMIDAZOLIUM; LIQUID FORMULATIONS; MACROPHAGE CELL LINE; METABOLIC ACTIVITY; MICROCALORIMETER; NEW APPROACHES; PROTEIN STABILITY; PROTEIN STABILIZATION; RESAZURIN; ROOM TEMPERATURE; SACCHARINATE; STRUCTURAL STABILITIES; THERAPEUTIC PROTEIN; UNFOLDING TEMPERATURE;

BIOCOMPATIBILITY; CELL CULTURE; ENZYMES; IONS; SODIUM; STABILITY;

IONIC LIQUIDS;

1 BUTYL 3 METHYL IMIDAZOLIUM LACTATE; CHOLINE DEHYDROGEN PHOSPHATE; CHOLINE SACCHARINATE; IONIC LIQUID; LYSOZYME; RESAZURIN; UNCLASSIFIED DRUG; BIOMATERIAL; BIOPOLYMER; PROTEIN;

ANIMAL CELL; AQUEOUS SOLUTION; ARTICLE; BIOCOMPATIBILITY; CELL CULTURE; CELL METABOLISM; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY TEST; DIFFERENTIAL SCANNING CALORIMETRY; DOSE RESPONSE; ENTHALPY; ENZYME ACTIVITY; FUNCTIONAL PROTEOMICS; MACROPHAGE; MOUSE; NONHUMAN; PROTEIN INTERACTION; PROTEIN STABILITY; PROTEIN STRUCTURE; TOXICITY TESTING; CHEMICAL MODEL; CHEMISTRY; COMPUTER SIMULATION; SOLUTION AND SOLUBILITY;

MICROCOCCUS;

BIOCOMPATIBLE MATERIALS; BIOPOLYMERS; COMPUTER SIMULATION; IONIC LIQUIDS; MODELS, CHEMICAL; PROTEINS; SOLUTIONS;

EID: 70349931524     PISSN: 01480731     EISSN: 15288951     Source Type: Journal    
DOI: 10.1115/1.3156810     Document Type: Article

References (16)

1. Baker, S. N., McCleskey, T. M., Pandey, S., and Baker, G. A., 2004, "Fluorescence Studies of Protein Thermostability in Ionic Liquids," Chem. Commun. (Cambridge), 8, pp. 940-941.
2. Fujita, K., MacFarlane, D. R., and Forsyth, M., 2005, "Protein Solubilising and Stabilizing Ionic Liquids," Chem. Commun. (Cambridge), 38, pp. 4804-4806.
3. Fujita, K., Forsyth, M., MacFarlane, D. R., Reid, R. W., and Elliott, G. D., 2006, "Unexpected Improvement in Stability and Utility of Cytochrome C by Solution in Biocompatible Ionic Liquids," Biotechnol. Bioeng., 94(6), pp. 1209-1213.
4. Byrne, N., Wang, L.-M., Belieres, J.-P., and Angell, C. A., 2007, "Reversible Folding-Unfolding, Aggregation Protection, and Multi-Year Stabilization, in High Concentration Protein Solutions, Using Ionic Liquids," Chem. Commun. (Cambridge), 26, pp. 2714-2716.
5. Cooper, A., Nutley, M. A., and Wadood, A., 2000, "Differential Scanning Microcalorimetry," Protein-Ligand Interactions: Hydrodynamics and Calorimetry, B. Z. Chowdhry and S. E. Harding, eds., Oxford University Press, Oxford, NY, pp. 287-318.
6. Spinozzi, F., Ortore, M. G., Sinibaldi, R., Mariani, P., Esposito, A., Cinelli, S., and Onori, G., 2008, "Microcalorimetric Study of Thermal Unfolding of Lysozyme in Water/Glycerol Mixtures: An Analysis by Solvent Exchange Model," J. Chem. Phys., 129, p. 035101.
7. Fraser, K. J., Izgordina, E. I., Forsyth, M., Scott, J. L., and MacFarlane, D. R., 2007, "Liquids Intermediate Between 'Molecular' and 'Ionic' Liquids: Liquid Ion Pairs?" Chem. Commun. (Cambridge), 37, pp. 3817-3819.
8. Ranke, J., Mölter, K., Stock, F., Bottin-Weber, U., Poczobutt, J., Hoffmann, J., Ondruschka, B., Filser, J., and Jastorff, B., 2004, "Biological Effects of Imidazolium Ionic Liquids With Varying Chain Lengths in Acute Vibrio Fischeri and WST-1 Cell Viability Assays," Ecotoxicol. Environ. Saf., 58, pp. 396-404.
9. 10 in Human Peripheral Blood Monocytes and J774 Macrophages," Respir. Res., 5(9), pp. 5-29.
10. Wright, E. L., Quenell, D. C., Suling, W. J., and Barrow, W. W., 1996, "Use of Mono Mac 6 Human Monocytic Line and J774 Murine Macrophage Cell Line in Parallel Antimycobacterial Drug Studies," Antimicrob. Agents Chemother., 40(9), pp. 2206-2208.
11. Jiang, G., Woo, B. H., Kang, F., Singh, J., and DeLuca, P. P., 2002, "Assessment of Protein Release Kinetics, Stability and Protein Polymer Interaction of Lysozyme Encapsulated Poly(D,L-lactide-co-glycolide) Microspheres," J. Controlled Release, 79, pp. 137-145.
12. Bradford, M., 1976, "A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding," Anal. Biochem., 72, pp. 248-254.
13. Shugar, D., 1952, "The Measurement of Lysozyme Activity and the Ultra- Violet Inactivation of Lysozyme," Biochim. Biophys. Acta, 8, pp. 302-309.
14. Stolte, S., Arning, J., Bottin-Weber, U., Matzke, M., Stock, F., Thiele, K., Uerdingen, M., Welz-Bierman, U., Jastorff, B., and Ranke, J., 2006, "Anion Effects on the Cytotoxicity of Ionic Liquids," Green Chem., 8, pp. 621-629.
15. Nockemann, P., Thijs, B., Driesen, K., Janssen, C. R., Van Hecke, K., Van Meervelt, L., Kossmann, S., Kirchner, B., and Binnemans, K., 2007, "Choline Saccharinate and Choline Acesulfamate: Ionic Liquids With Low Toxicities," J. Phys. Chem. B, 111, pp. 5254-5263.
16. Micaêlo, N. M., and Soares, C. M., 2008, "Protein Structure and Dynamics in Ionic Liquids. Insights from Molecular Simulation Studies," J. Phys. Chem. B, 112, pp. 2566-2572.