Association studies of erythoid α-spectrin at the tetramerization site

British Journal of Haematology

Volumn 147, Issue 3, 2009, Pages 392-395

  Lam, Vinh Q ^a   Antoniou, Chloe ^a   Rolius, Ramunas ^a   Fung, Leslie W M ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

Author keywords

Hereditary elliptocytosis; Hereditary pyropoikilocytosis; Protein protein interactions; Spectrin; Tetramer

Indexed keywords

FODRIN; PROTEIN BETA SPECTRIN; SPECTRIN; TETRAMER; UNCLASSIFIED DRUG; PEPTIDE FRAGMENT;

ARTICLE; CIRCULAR DICHROISM; CLINICAL FEATURE; ERYTHROCYTE; HEREDITARY ELLIPTOCYTOSIS; HEREDITARY HEMOLYTIC ANEMIA; HUMAN; MUTATION; PRIORITY JOURNAL; PYROPOIKILOCYTOSIS; CHEMISTRY; ELECTRON SPIN RESONANCE; GENETICS; METHODOLOGY; PROTEIN BINDING; STRUCTURE ACTIVITY RELATION;

ELECTRON SPIN RESONANCE SPECTROSCOPY; ERYTHROCYTES; HUMANS; MUTATION; PEPTIDE FRAGMENTS; PROTEIN BINDING; SPECTRIN; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 70349920964     PISSN: 00071048     EISSN: 13652141     Source Type: Journal    
DOI: 10.1111/j.1365-2141.2009.07876.x     Document Type: Article

References (15)

1. Antoniou, C., Lam, V.Q. Fung, L.W.-M. (2008) Conformational changes at the tetramerization site of erythroid α-spectrin upon binding β-spectrin: a spin label EPR study. Biochemistry, 47, 10765 10772.
2. Bennett, V. (1989) The spectrin-actin junction of erythrocyte membrane skeletons. Biochimica et Biophysica Acta, 988, 107 121.
3. Bignone, P.A. Baines, A.J. (2003) Spectrin aII and bII isoforms interact with high affinity at the tetramerization site. Biochemical Journal, 374, 613 624.
4. Bolin, K.A., Hanson, P., Wright, S.J. Millhauser, G.L. (1998) An NMR investigation of the conformational effect of nitroxide spin labels on ala-rich helical peptides. Journal of Magnetic Resonance, 131, 248 253.
5. Delaunay, J. (2007) The molecular basis of hereditary red cell membrane disorders. Blood Reviews, 21, 1 20.
6. Gaetani, M., Mootien, S., Harper, S., Gallagher, P.G. Speicher, D.W. (2008) Structural and functional effects of hereditary hemolytic anemia-associated point mutations in the alpha spectrin tetramer site. Blood, 111, 5712 5720.
7. Hustedt, E.J. Beth, A.H. (1999) Nitroxide spin-spin interactions: applications to protein structure and dynamics. Annual Review of Biophysics and Biomolecular Structure, 28, 129 153.
8. Li, Q. Fung, L.W.-M. (2009) Structural and dynamic study of the tetramerization region of non-erythroid a-spectrin: a frayed helix revealed by site-directed spin labeling electron paramagnetic resonance. Biochemistry, 48, 206 215.
9. Mehboob, S., Luo, B.-H., Fu, W., Johnson, M.E. Fung, L.W.-M. (2005) Conformational studies of the tetramerization site of human erythroid spectrin by cysteine-scanning spin labeling EPR methods. Biochemistry, 44, 15898 15905.
10. Nicolas, G., Pedroni, S., Fournier, C., Gautero, H., Craescu, C., Dhermy, D. Lecomte, M.C. (1998) Spectrin self-association site: characterization and study of beta-spectrin mutations associated with hereditary elliptocytosis. Biochemical Journal, 332, 81 89.
11. Park, S., Johnson, M.E. Fung, L.W.-M. (2002) Nuclear magnetic resonance studies of mutations of the tetramerization region of human alpha spectrin. Blood, 100, 283 288.
12. Park, S., Caffrey, M.S., Johnson, M.E. Fung, L.W.-M. (2003) Solution structural studies on human erythrocyte a-spectrin tetramerization site. Journal of Biological Chemistry, 278, 21837 21844.
13. Pothier, B., Alloisio, N., Marechal, J., Morle, L., Ducluzeau, M.T., Caldani, C., Philippe, N. Delaunay, J. (1990) Assignment of Sp I/74 hereditary elliptocytosis to the α- or β-chain of spectrin through in vitro dimer reconstitution. Blood, 75, 2061 2069.
14. Song, Y., Pipalia, N. Fung, L.W.-M. (2009) The L49F mutation in alpha erythroid spectrin induces local disorder in the tetramer association region: fluorescence and molecular dynamics studies of free and bound alpha spectrin. Protein Science, 18, 1916 1925.
15. Speicher, D.W., DeSilva, T.M., Speicher, K.D.J., Ursitti, A., Hembach, P. Weglarz, L. (1993) Location of the human red cell spectrin tetramer binding site and detection of a related 'closed' hairpin loop dimer using proteolytic footprinting. Journal of Biological Chemistry, 268, 4227 4235.