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Volumn 23, Issue 4, 2008, Pages 306-312

Quantification of microcystis in a eutrophic lake by simple DNA extraction and SYBR green real-time PCR

Author keywords

DNA extraction; Eutrophic lake; Microcystis; Quantification; SYBR Green I

Indexed keywords

ALGAE; MICROCYSTIS;

EID: 70349915871     PISSN: 13426311     EISSN: 13426311     Source Type: Journal    
DOI: 10.1264/jsme2.ME08515     Document Type: Article
Times cited : (16)

References (39)
  • 1
    • 33746583201 scopus 로고    scopus 로고
    • Potential pitfalls in the quantitative molecular detection of escherichia coli O157:H7 in environmental matrices
    • Artz, R.R.E., L.M. Avery, D.L. Jones, and K. Killham. 2006. Potential pitfalls in the quantitative molecular detection of Escherichia coli O157:H7 in environmental matrices. Can. J. Microbiol. 52:482-488.
    • (2006) Can. J. Microbiol , Issue.52 , pp. 482-488
    • Artz, R.R.E.1    Avery, L.M.2    Jones, D.L.3    Killham, K.4
  • 2
    • 0036946724 scopus 로고    scopus 로고
    • Monitoring changing toxigenicity of a cyanobacterial bloom by molecular methods
    • Baker, J.A., B. Entsch, B.A. Neilan, and D.B. McKay. 2002. Monitoring changing toxigenicity of a cyanobacterial bloom by molecular methods. Appl. Environ. Microbiol. 68:6070-6076.
    • (2002) Appl. Environ. Microbiol , vol.68 , pp. 6070-6076
    • Baker, J.A.1    Entsch, B.2    Neilan, B.A.3    McKay, D.B.4
  • 3
    • 3543006508 scopus 로고    scopus 로고
    • Growth rate regulation of rRNA content of a marine synechococcus (cyanobacterium) strain
    • Binder, B.J., and Y.C. Liu. 1998. Growth rate regulation of rRNA content of a marine Synechococcus (cyanobacterium) strain. Appl. Environ. Microbiol. 64:3346-3351.
    • (1998) Appl. Environ. Microbiol , vol.64 , pp. 3346-3351
    • Binder, B.J.1    Liu, Y.C.2
  • 4
    • 61849131560 scopus 로고    scopus 로고
    • CGER. Center for Global Environmental Research, National Institute for Environmental Studies, Tukuba, Japan
    • CGER. 2007. Lake Kasumigaura database. Center for Global Environmental Research, National Institute for Environmental Studies, Tukuba, Japan. http://db.cger.nies.go.jp/gem/inter/GEMS/database/ kasumi/top.html.
    • (2007) Lake Kasumigaura database
  • 5
    • 0022610857 scopus 로고
    • Role of ribosome degradation in the death of starved escherichia coli cells
    • Davis, B.D., S.M. Luger, and P.C. Tai. 1986. Role of ribosome degradation in the death of starved Escherichia coli cells. J. Bacteriol. 166:439-445.
    • (1986) J. Bacteriol , vol.166 , pp. 439-445
    • Davis, B.D.1    Luger, S.M.2    Tai, P.C.3
  • 7
    • 26644464141 scopus 로고    scopus 로고
    • Using Dirichlet tessellation to help estimate microbial biomass concentrations
    • Dugan, N.R. 2005. Using Dirichlet tessellation to help estimate microbial biomass concentrations. J. Microbiol. Methods 63:205-210.
    • (2005) J. Microbiol. Methods , vol.63 , pp. 205-210
    • Dugan, N.R.1
  • 8
    • 33749267987 scopus 로고    scopus 로고
    • Highly sensitive real-time PCR assay for quantification of toxic cyanobacteria based on microcystin synthetase A gene
    • Furukawa, K., N. Noda, S. Tsuneda, T. Saito, T. Itayama, and Y. Inamori. 2006. Highly sensitive real-time PCR assay for quantification of toxic cyanobacteria based on microcystin synthetase A gene. J. Biosci. Bioeng. 102:90-96.
    • (2006) J. Biosci. Bioeng , vol.102 , pp. 90-96
    • Furukawa, K.1    Noda, N.2    Tsuneda, S.3    Saito, T.4    Itayama, T.5    Inamori, Y.6
  • 9
    • 0025322547 scopus 로고
    • Tangential flow filtration and preliminary phylogenetic analysis of Marine Picoplankton
    • Giovannoni, S.J., E.F. Delong, T.M. Schmidt, and N.R. Pace. 1990. Tangential flow filtration and preliminary phylogenetic analysis of marine picoplankton. Appl. Environ. Microbiol. 56:2572-2575.
    • (1990) Appl. Environ. Microbiol , vol.56 , pp. 2572-2575
    • Giovannoni, S.J.1    Delong, E.F.2    Schmidt, T.M.3    Pace, N.R.4
  • 10
    • 33845930902 scopus 로고    scopus 로고
    • Interactive influences of nutrient loading, zooplankton grazing, and micro-cystin synthetase gene expression on cyanobacterial bloom dynamics in a eutrophic New York lake
    • Gobler, C.J., T.W. Davis, K.J. Coyne, and G.L. Boyer. 2007. Interactive influences of nutrient loading, zooplankton grazing, and micro-cystin synthetase gene expression on cyanobacterial bloom dynamics in a eutrophic New York lake. Harmful Algae 6:119-133.
    • (2007) Harmful Algae , vol.6 , pp. 119-133
    • Gobler, C.J.1    Davis, T.W.2    Coyne, K.J.3    Boyer, G.L.4
  • 14
    • 0000166036 scopus 로고    scopus 로고
    • Detection and 16S rDNA sequence analysis of a bloom-forming cyanobacterial genus
    • Kondo, R., M. Komura, S. Hiroishi, and Y. Hata. 1998. Detection and 16S rDNA sequence analysis of a bloom-forming cyanobacterial genus Microcystis. Fisheries Sci. 64:840-841.
    • (1998) Microcystis. Fisheries Sci , vol.64 , pp. 840-841
    • Kondo, R.1    Komura, M.2    Hiroishi, S.3    Hata, Y.4
  • 15
    • 0001857117 scopus 로고
    • 16S/23S RRNA sequencing
    • E. Stackebrandt and M. Goodfellow (ed.). John Wiley & Sons, Chichester, United Kingdom
    • Lane, D.J. 1991. 16S/23S rRNA sequencing, p. 115-175. In E. Stackebrandt and M. Goodfellow (ed.), Nucleic Acid Techniques in Bacterial Systematics. John Wiley & Sons, Chichester, United Kingdom.
    • (1991) Nucleic Acid Techniques in Bacterial Systematics , pp. 115-175
    • Lane, D.J.1
  • 16
    • 10744219802 scopus 로고    scopus 로고
    • Quantitative real-time PCR using TaqMan and SYBR Green for actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, tetq gene and total bacteria
    • Maeda, H., C. Fujimoto, Y. Haruki, T. Maeda, S. Kokeguchi, M. Petelin, H. Arai, I. Tanimoto, F. Nishimura, and S. Takashiba. 2003. Quantitative real-time PCR using TaqMan and SYBR Green for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, tetQ gene and total bacteria. FEMS Immunol. Med. Microbiol. 39:81-86.
    • (2003) FEMS Immunol. Med. Microbiol , vol.39 , pp. 81-86
    • Maeda, H.1    Fujimoto, C.2    Haruki, Y.3    Maeda, T.4    Kokeguchi, S.5    Petelin, M.6    Arai, H.7    Tanimoto, I.8    Nishimura, F.9    Takashiba, S.10
  • 17
    • 0242417422 scopus 로고    scopus 로고
    • Natural variation in the microcystin synthetase operon mcyABC and impact on micro- cystin production in Microcystis Strains
    • Mikalsen, B., G. Boison, O.M. Skulberg, J. Fastner, W. Davies, T.M. Gabrielsen, K. Rudi, and K. S. Jakobsen. 2003. Natural variation in the microcystin synthetase operon mcyABC and impact on micro- cystin production in Microcystis strains. J. Bacteriol. 185:2774-2785.
    • (2003) J. Bacteriol , vol.185 , pp. 2774-2785
    • Mikalsen, B.1    Boison, G.2    Skulberg, O.M.3    Fastner, J.4    Davies, W.5    Gabrielsen, T.M.6    Rudi, K.7    Jakobsen, K.S.8
  • 18
    • 1842509857 scopus 로고    scopus 로고
    • Convenient determination of DNA extraction efficiency using an external DNA recovery standard and quantitative-competitive PCR
    • Mumy, K.L., and R.H. Findlay. 2004. Convenient determination of DNA extraction efficiency using an external DNA recovery standard and quantitative-competitive PCR. J. Microbiol. Methods 57:259-268.
    • (2004) J. Microbiol. Methods , vol.57 , pp. 259-268
    • Mumy, K.L.1    Findlay, R.H.2
  • 19
    • 33750941233 scopus 로고    scopus 로고
    • Effect of iron complexation with dissolved organic matter on the growth of cyanobacteria in a eutrophic lake
    • Nagai, T., A. Imai, K. Matsushige, and T. Fukushima. 2006. Effect of iron complexation with dissolved organic matter on the growth of cyanobacteria in a eutrophic lake. Aquat. Microb. Ecol. 44:231-239.
    • (2006) Aquat. Microb. Ecol , vol.44 , pp. 231-239
    • Nagai, T.1    Imai, A.2    Matsushige, K.3    Fukushima, T.4
  • 20
    • 37549018436 scopus 로고    scopus 로고
    • Growth characteristics and growth modeling of microcystis aeruginosa and planktothrix agardhii under iron limitation
    • Nagai, T., A. Imai, K. Matsushige, and T. Fukushima. 2007. Growth characteristics and growth modeling of Microcystis aeruginosa and Planktothrix agardhii under iron limitation. Limnology 8:261-270.
    • (2007) Limnology , vol.8 , pp. 261-270
    • Nagai, T.1    Imai, A.2    Matsushige, K.3    Fukushima, T.4
  • 22
    • 1642482669 scopus 로고    scopus 로고
    • Three-dimensional differentiation of photo-autotrophic biofilm constituents by multi-channel laser scanning microscopy (single-photon and two-photon excitation
    • Neu, T.R., S. Woelfl, and J.R. Lawrence. 2004. Three-dimensional differentiation of photo-autotrophic biofilm constituents by multi-channel laser scanning microscopy (single-photon and two-photon excitation). J. Microbiol. Methods 56:161-172.
    • (2004) J. Microbiol. Methods , vol.56 , pp. 161-172
    • Neu, T.R.1    Woelfl, S.2    Lawrence, J.R.3
  • 23
    • 33750080742 scopus 로고    scopus 로고
    • Blooms of single bacterial species in a coastal lagoon of the southwestern Atlantic Ocean
    • Piccini, C., D. Conde, C. Alonso, R. Sommaruga, and J. Pernthaler. 2006. Blooms of single bacterial species in a coastal lagoon of the southwestern Atlantic Ocean. Appl. Environ. Microbiol. 72:6560-6568.
    • (2006) Appl. Environ. Microbiol , vol.72 , pp. 6560-6568
    • Piccini, C.1    Conde, D.2    Alonso, C.3    Sommaruga, R.4    Pernthaler, J.5
  • 24
    • 0030330669 scopus 로고    scopus 로고
    • Effects of nutrient media and culture duration on growth, macromolecular composition and toxicity in batch cultures of microcystis aeruginosa
    • Rao, P.V.L., A.S.B. Bhaskar, and R. Bhattacharya. 1996. Effects of nutrient media and culture duration on growth, macromolecular composition and toxicity in batch cultures of Microcystis aeruginosa. Microbios 86:95-104.
    • (1996) Microbios , vol.86 , pp. 95-104
    • Rao, P.V.L.1    Bhaskar, A.S.B.2    Bhattacharya, R.3
  • 25
    • 20044384537 scopus 로고    scopus 로고
    • Quantification of toxic micro- cystis spp. during the 2003 and 2004 blooms in western lake erie using quantitative real-time PCR
    • Rinta-Kanto, J.M., A.J.A. Ouellette, G.L. Boyer, M.R. Twiss, T.B. Bridgeman, and S.W. Wilhelm. 2005. Quantification of toxic Micro- cystis spp. during the 2003 and 2004 blooms in western Lake Erie using quantitative real-time PCR. Environ. Sci. Technol. 39:4198-4205.
    • (2005) Environ. Sci. Technol , vol.39 , pp. 4198-4205
    • Rinta-Kanto, J.M.1    Ouellette, A.J.A.2    Boyer, G.L.3    Twiss, M.R.4    Bridgeman, T.B.5    Wilhelm, S.W.6
  • 26
    • 1842716750 scopus 로고    scopus 로고
    • Three-dimensional modelling of a microcystis bloom event in the Swan River estuary, Western Australia
    • Robson, B.J., and D.P. Hamilton. 2004. Three-dimensional modelling of a Microcystis bloom event in the Swan River estuary, Western Australia. Ecol. Model. 174:203-222.
    • (2004) Ecol. Model , vol.174 , pp. 203-222
    • Robson, B.J.1    Hamilton, D.P.2
  • 27
    • 33748627689 scopus 로고    scopus 로고
    • Automatic analysis of aqueous specimens for phytoplankton structure recognition and population estimation
    • Rodenacker, K., B. Hense, U. Jütting, and P. Gais. 2006. Automatic analysis of aqueous specimens for phytoplankton structure recognition and population estimation. Microsc. Res. Tech. 69:708-720.
    • (2006) Microsc. Res. Tech , vol.69 , pp. 708-720
    • Rodenacker, K.1    Hense, B.2    Jütting, U.3    Gais, P.4
  • 28
    • 0033823572 scopus 로고    scopus 로고
    • Application of sequence-specific labeled 16s rrna gene oligonucle-otide probes for genetic profiling of cyanobacterial abundance and diversity by array hybridization
    • Rudi, K., O.M. Skulberg, R. Skulberg, and K.S. Jakobsen. 2000. Application of sequence-specific labeled 16S rRNA gene oligonucle- otide probes for genetic profiling of cyanobacterial abundance and diversity by array hybridization. Appl. Environ. Microbiol. 66:4004-4011.
    • (2000) Appl. Environ. Microbiol. , vol.66 , pp. 4004-4011
    • Rudi, K.1    Skulberg, O.M.2    Skulberg, R.3    Jakobsen, K.S.4
  • 29
    • 0023375195 scopus 로고
    • The neighbor-joining method: a new method for reconstructing phylogenetic trees
    • Saitou, N., and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425.
    • (1987) Mol. Biol. Evol , vol.4 , pp. 406-425
    • Saitou, N.1    Nei, M.2
  • 30
    • 33748672719 scopus 로고    scopus 로고
    • Relationship among several key cell cycle events in the developmental cyanobacte- rium anabaena sp strain PCC 7120
    • Sakr, S., M. Thyssen, M. Denis, and C.-C. Zhang. 2006. Relationship among several key cell cycle events in the developmental cyanobacte- rium Anabaena sp. strain PCC 7120. J. Bacteriol. 188:5958-5965.
    • (2006) J. Bacteriol , vol.188 , pp. 5958-5965
    • Sakr, S.1    Thyssen, M.2    Denis, M.3    Zhang, C.-C.4
  • 32
    • 0000347899 scopus 로고
    • Phyto-plankton species shift accompanied by transition from nitrogen dependence to phosphorus dependence of primary production in Lake Kasumigaura, Japan
    • Takamura, N., A. Otsuki, M. Aizaki, and Y. Nojiri. 1992. Phyto-plankton species shift accompanied by transition from nitrogen dependence to phosphorus dependence of primary production in Lake Kasumigaura, Japan. Arch. Hydrobiol. 124:129-148.
    • (1992) Arch. Hydrobiol , vol.124 , pp. 129-148
    • Takamura, N.1    Otsuki, A.2    Aizaki, M.3    Nojiri, Y.4
  • 33
    • 0027968068 scopus 로고
    • Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
    • Thompson, J.D., D.G. Higgins, and T.J. Gibson. 1994. Clustal W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680.
    • (1994) Nucleic Acids Res , vol.22 , pp. 4673-4680
    • Thompson, J.D.1    Higgins, D.G.2    Gibson, T.J.3
  • 34
    • 0035376988 scopus 로고    scopus 로고
    • Detection of toxi- genicity by a probe for the microcystin synthetase A gene (mcyA) of the cyanobacterial genus microcystis, comparison of toxicities with 16S rRNA and phycocyanin operon (phycocyanin intergenic spacer) phylogenies
    • Tillett, D., D.L. Parker, and B.A. Neilan. 2001. Detection of toxi- genicity by a probe for the microcystin synthetase A gene (mcyA) of the cyanobacterial genus Microcystis, comparison of toxicities with 16S rRNA and phycocyanin operon (phycocyanin intergenic spacer) phylogenies. Appl. Environ. Microbiol. 67:2810-2818.
    • (2001) Appl. Environ. Microbiol , vol.67 , pp. 2810-2818
    • Tillett, D.1    Parker, D.L.2    Neilan, B.A.3
  • 35
    • 0036066028 scopus 로고    scopus 로고
    • Fluorescence- assisted image analysis of freshwater microalgae
    • Walker, R.F., K. Ishikawa, and M. Kumagai. 2002. Fluorescence- assisted image analysis of freshwater microalgae. J. Microbiol. Methods 51:149-162.
    • (2002) J. Microbiol. Methods , vol.51 , pp. 149-162
    • Walker, R.F.1    Ishikawa, K.2    Kumagai, M.3
  • 36
    • 0003379912 scopus 로고
    • The Cyanobacteria: Isolation, purification, and identification
    • In A. Balows, H.G. Truper, M. Dworkin, W. Harder, and K.H. Schleifer (ed.) Springer, New York
    • Waterbury, J.B. 1992. The Cyanobacteria: Isolation, purification, and identification, p. 2058-2078. In A. Balows, H.G. Truper, M. Dworkin, W. Harder, and K.H. Schleifer (ed.), The Prokaryotes, 2nd ed. Springer, New York.
    • (1992) The Prokaryotes, 2nd Ed. , pp. 2058-2078
    • Waterbury, J.B.1
  • 37
    • 0037403162 scopus 로고    scopus 로고
    • The low TN:TP ratio, a cause or a result of microcystis blooms?
    • Xie, L., P. Xie, S. Li, H. Tang, and H. Liu. 2003. The low TN:TP ratio, a cause or a result of Microcystis blooms? Water Res. 37:2073-2080.
    • (2003) Water Res , vol.37 , pp. 2073-2080
    • Xie, L.1    Xie, P.2    Li, S.3    Tang, H.4    Liu, H.5
  • 38
    • 24944540267 scopus 로고    scopus 로고
    • Coordination of DNA replication and cell division in cyanobacteria micro- cystis aeruginosa
    • Yoshida, T., M. Maki, H. Okamoto, and S. Hiroishi. 2005. Coordination of DNA replication and cell division in cyanobacteria Micro- cystis aeruginosa. FEMS Microbiol. Let. 251:149-154.
    • (2005) FEMS Microbiol. Let , vol.251 , pp. 149-154
    • Yoshida, T.1    Maki, M.2    Okamoto, H.3    Hiroishi, S.4
  • 39
    • 33750734678 scopus 로고    scopus 로고
    • Cyanobacterium microcystis bloom in a eutrophicated regulating reservoir
    • Yoshinaga, I., T. Hitomi, A. Miura, E. Shiratani, and T. Miyazaki. 2006. Cyanobacterium Microcystis bloom in a eutrophicated regulating reservoir. JARQ 40:283-289.
    • (2006) JARQ , vol.40 , pp. 283-289
    • Yoshinaga, I.1    Hitomi, T.2    Miura, A.3    Shiratani, E.4    Miyazaki, T.5


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