Real Time PCR TaqMan assays for detection of polyomaviruses KIV and WUV in clinical samples

Journal of Virological Methods

Volumn 162, Issue 1-2, 2009, Pages 69-74

  Bergallo, Massimiliano ^a   Terlizzi, Maria Elena ^a   Astegiano, Sara ^a   Ciotti, Marco ^b   Babakir Mina, Muhammed ^b   Perno, Carlo Federico ^b   Cavallo, Rossana ^a   Costa, Cristina ^a  

^a UNIVERSITY OF TURIN   (Italy)

^b UNIVERSITY OF ROME TOR VERGATA   (Italy)

Author keywords

Polyomavirus KI; Polyomavirus WU; Primer probe set; Real Time polymerase chain reaction

Indexed keywords

VIRUS DNA;

ARTICLE; CONTROLLED STUDY; DNA DETERMINATION; FECES ANALYSIS; HUMAN; HUMAN TISSUE; KAROLINSKA INSTITUTET VIRUS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYOMA VIRUS; PRIORITY JOURNAL; PROCESS OPTIMIZATION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; TONSIL; VIRUS DETECTION; WASHINGTON UNIVERSITY VIRUS;

DNA PRIMERS; DNA, VIRAL; FECES; HUMANS; PALATINE TONSIL; POLYOMAVIRUS; POLYOMAVIRUS INFECTIONS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SPECIES SPECIFICITY; TAQ POLYMERASE; TUMOR VIRUS INFECTIONS;

POLYOMAVIRUS;

EID: 70349728256     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2009.07.016     Document Type: Article

References (16)

1. Allander T., Andreasson K., Gupta S., Bjerkner A., Bogdanovic G., Persson M.A., Dalianis T., Ramqvist T., and Andersson B. Identification of a third human polyomavirus. J. Virol. 81 (2007) 4130-4136
2. Babakir-Mina M., Ciccozzi M., Dimonte S., Farchi F., Valdarchi C., Rezza G., Perno C.F., and Ciotti M. Identification of the novel KI polyomavirus in the respiratory tract of an Italian patient. J. Med. Virol. 80 (2008) 2012-2014
3. Babakir-Mina M., Ciccozzi M., Campitelli L., Aquaro S., Lo Coco A., Perno C.F., and Ciotti M. Identification of the novel KI polyomavirus in paranasal and lung tissues. J. Med. Virol. 81 (2009) 558-561
4. Bergallo M., Costa C., Margio S., Sidoti F., Segoloni G.P., Ponzi A.N., and Cavallo R. Detection and typing of BKV, JCV, and SV40 by multiplex nested polymerase chain reaction. Mol. Biotechnol. 35 (2007) 243-252
5. Bialasiewicz S., Whiley D.M., Lambert S.B., Gould A., Nissen M.D., and Sloots T.P. Development and evaluation of real-time PCR assays for the detection of the newly identified KI and WU polyomaviruses. J. Clin. Virol. 40 (2007) 9-14
6. Butel J.S., and Lednicky J.A. Cell and molecular biology of simian virus 40: implications for human infections and disease. J. Natl. Cancer Inst. 91 (1999) 119-134
7. Costa C., Bergallo M., Astegiano S., Terlizzi M.E., Sidoti F., Segoloni G.P., and Cavallo R. Monitoring of BK virus replication in the first year following renal transplantation. Nephrol. Dial. Transplant. 23 (2008) 3333-3336
8. Dimech W., Bowden D.S., Brestovac B., Byron K., James G., Jardine D., Sloots T., and Dax E.M. Validation of assembled nucleic acid-based tests in diagnostic microbiology laboratories. Pathology 36 (2004) 45-50
9. Gardner S.D., Field A.M., Coleman D.V., and Hulme B. New human papovavirus (BK) isolated from urine after renal transplantation. Lancet 1 (1971) 1253-1257
10. Gaynor A.M., Nissen M.D., Whiley D.M., Mackay I.M., Lambert S.B., Wu G., Brennan D.C., Storch G.A., Sloots T.P., and Wang D. Identification of a novel polyomavirus from patients with acute respiratory tract infections. PLoS Pathog. 3 (2007) e64
11. Jiang M., Abend J.R., Johnson S.F., and Imperiale M.J. The role of polyomaviruses in human disease. Virology 384 (2009) 266-273
12. Lindau C., Tiveljung-Lindell A., Goh S., Ramqvist T., and Allander T. A single-tube, real-time PCR assay for detection of two newly characterized human KI and WU polyomaviruses. J. Clin. Virol. 44 (2009) 24-26
13. Norja P., Ubillos I., Templeton K., and Simmonds P. No evidence for an association between infections with WU and KI polyomaviruses and respiratory disease. J. Clin. Virol. 40 (2007) 307-311
14. Padgett B.L., Walker D.L., ZuRhein G.M., Eckroade R.J., and Dessel B.H. Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy. Lancet 1 (1971) 1257-1260
15. Payungporn S., Chieochansin T., Thongmee C., Panjaworayan N., Samransamruajkit R., Theamboolers A., and Poovorawan Y. Detection and discrimination of WU/KI polyomaviruses by real-time PCR with melting curve analysis. J. Virol. Methods 153 (2008) 70-73
16. Rabenau H.F., Kessler H.H., Kortenbusch M., Steinhorst A., Raggam R.B., and Berger A. Verification and validation of diagnostic laboratory tests in clinical virology. J. Clin. Virol. 40 (2007) 93-98