Measurement of ribosomal RNA turnover in vivo by use of deuterium-labeled glucose

Clinical Chemistry

Volumn 55, Issue 10, 2009, Pages 1824-1833

  Defoiche, Julien ^a,b   Zhang, Yan ^a   Lagneaux, Laurence ^c   Pettengell, Ruth ^a   Hegedus, Andrea ^a   Willems, Luc ^b   Macallan, Derek C ^a  

^a ST GEORGE'S UNIVERSITY OF LONDON   (United Kingdom)

^b UNIVERSITY OF LIÈGE   (Belgium)

^c UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

CD3 ANTIGEN; DEUTERIUM; GLUCOSE; RIBOSOME RNA;

ADULT; ANALYTIC METHOD; ARTICLE; B LYMPHOCYTE; CASE REPORT; CELL LABELING; CELL PROLIFERATION; CHRONIC LYMPHATIC LEUKEMIA; DERIVATIZATION; DNA METABOLISM; HUMAN; HUMAN CELL; HYDROLYSIS; IN VIVO STUDY; ISOTOPE LABELING; LYMPH NODE METASTASIS; LYMPHOCYTE CULTURE; LYMPHOID CELL LINE; MALE; MASS FRAGMENTOGRAPHY; RNA EXTRACTION; RNA METABOLISM; RNA SYNTHESIS;

ADENOSINE; AIDS-RELATED COMPLEX; CELL LINE, TUMOR; CELL PROLIFERATION; DEUTERIUM; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GLUCOSE; HUMANS; LEUKEMIA, LYMPHOCYTIC, CHRONIC, B-CELL; LYMPH NODES; LYMPHOCYTES; LYMPHOMA, NON-HODGKIN; MALE; MIDDLE AGED; MODELS, BIOLOGICAL; RIBONUCLEOSIDES; RNA, RIBOSOMAL; SARCOMA, KAPOSI;

TETRA;

EID: 70349684819     PISSN: 00099147     EISSN: 15308561     Source Type: Journal    
DOI: 10.1373/clinchem.2008.119446     Document Type: Article

References (28)

1. Ross J. mRNA stability in mammalian cells. Microbiol Rev 1995;59:423-450
2. Gillery P, Georges N, Wegrowski J, Randoux A, Borel JP. Protein synthesis in collagen lattice-cultured fibroblasts is controlled at the ribosomal level. FEBS Lett 1995;357:287-289
3. Yi X, Tesmer VM, Savre-Train I, Shay JW, Wright WE. Both transcriptional and posttranscriptional mechanisms regulate human telomerase template RNA levels. Mol Cell Biol 1999;19:3989-3997 (Pubitemid 29241973)
4. Halle JP, Muller S, Simm A, Adam G. Copy number, epigenetic state and expression of the rRNA genes in young and senescent rat embryo fibroblasts. Eur J Cell Biol 1997;74:281-288 (Pubitemid 27490219)
5. Gerber G, Gerber G, Altman KI. Studies on the metabolism of tissue proteins, I: turnover of collagen labeled with proline-U-C14 in young rats. J Biol Chem 1960;235:2653-2656
6. Cooper HL. Studies on RNA metabolism during lymphocyte activation.Transplant Rev 1972;11:3-38.
7. Grimble GK, Malik SB, Boza JJ. Methods for measuring tissue RNA turnover. Curr Opin Clin Nutr Metab Care 2000;3:399-408.
8. Ruggero D, Pandolfi PP. Does the ribosome translate cancer? Nat Rev Cancer 2003;3:179-192
9. Rubin AD. Defective control of ribosomal RNA processing in stimulated leukemic lymphocytes. J Clin Invest 1971;50:2485-2497
10. Busch R, Neese RA, Awada M, Hayes GM, Hellerstein MK. Measurement of cell proliferation by heavy water labeling. Nat Protoc 2007;2:3045-3057
11. Macallan DC, Fullerton CA, Neese RA, Haddock K, Park S, Hellerstein MK. Measurement of cell proliferation by labeling of DNA with stable isotope-labeled glucose: studies in vitro, in animals and in humans. Proc Natl Acad Sci U S A 1998;95:708-713 (Pubitemid 28083808)
12. Macallan DC, Asquith B, Zhang Y, de Lara CM, Ghattas H, Defoiche J, Beverley PC. Measurement of proliferation and disappearance of rapid turnover cell populations in human studies using deuterium-labelled glucose. Nat Protoc 2009;4:1313-1327
13. Neese RA, Siler SQ, Cesar D, Antelo F, Lee D, Misell L, et al. Advances in the stable isotope-mass spectrometric measurement of DNA synthesis and cell proliferation. Anal Biochem 2001;298:189-195 (Pubitemid 33105233)
14. Neese RA, Misell LM, Turner S, Chu A, Kim J, Cesar D, et al. Measurement in vivo of proliferation rates of slow turnover cells by 2H2O labeling of the deoxyribose moiety of DNA. Proc Natl Acad Sci U S A 2002;99:15345-15350 (Pubitemid 35403937)
15. Chu A, Ordonez ET, Hellerstein MK. Measurement of mouse vascular smooth muscle and atheroma cell proliferation by 2H2O incorporation into DNA. Am J Physiol Cell Physiol 2006;291:C1014-C1021. (Pubitemid 44771819)
16. Defoiche J, Debacq C, Asquith B, Zhang Y, Burny A, Bron D, et al. Reduction of B cell turnover in chronic lymphocytic leukaemia. Br J Haematol 2008;143:240-247
17. Messmer BT, Messmer D, Allen SL, Kolitz JE, Kudalkar P, Cesar D, et al. In vivo measurements document the dynamic cellular kinetics of chronic lymphocytic leukemia B cells. J Clin Invest 2005;115:755-764
18. McCune JM, Hanley MB, Cesar D, Halvorsen R, Hoh R, Schmidt D, et al. Factors influencing T-cell turnover in HIV-1-seropositive patients. J Clin Invest 2000;105:R1-8. (Pubitemid 30146516)
19. Hellerstein M, Hanley MB, Cesar D, Siler S, Papageorgopoulos C, Wieder E, et al. Directly measured kinetics of circulating T lymphocytes in normal and HIV-1-infected humans. Nat Med 1999;5:83-89 (Pubitemid 29051003)
20. Asquith B, Zhang Y, Mosley AJ, de Lara CM, Wallace DL, Worth A, et al. In vivo T lymphocyte dynamics in humans and the impact of human T-lymphotropic virus 1 infection. Proc Natl Acad Sci U S A 2007;104:8035-8040
21. Ghattas H, Darboe BM, Wallace DL, Griffin GE, Prentice AM, Macallan DC. Measuring lymphocyte kinetics in tropical field settings. Trans R Soc Trop Med Hyg 2005;99:675-685 (Pubitemid 40945757)
22. Crain PF. Preparation and enzymatic hydrolysis of DNA and RNA for mass spectrometry. Methods Enzymol 1990;193:782-790
23. Asquith B, Debacq C, Macallan DC, Willems L, Bangham C. Lymphocyte kinetics: the interpretation of labelling data. Trends Immunol 2002;23:596-601. (Pubitemid 35449099)
24. Macallan DC, Asquith B, Irvine A, Wallace D, Worth A, Ghattas H, et al. Measurement and modeling of human T cell kinetics. Eur J Immunol 2003;33:2316-2326 (Pubitemid 37010049)
25. Boros LG, Steinkamp MP, Fleming JC, Lee WN, Cascante M, Neufeld EJ. Defective RNA ribose synthesis in fibroblasts from patients with thiamine-responsive megaloblastic anemia (TRMA). Blood 2003;102:3556-3561 (Pubitemid 37409370)
26. Cohen A, Barankiewicz J, Lederman HM, Gelfand EW. Purine and pyrimidine metabolism in human T lymphocytes: regulation of deoxyribonucleotide metabolism. J Biol Chem 1983;258:12334-12340 (Pubitemid 14230021)
27. Reichard P. Interactions between deoxyribonucleotide and DNA synthesis. Annu Rev Biochem 1988;57:349-374
28. Voogt JN, Awada M, Murphy EJ, Hayes GM, Busch R, Hellerstein MK. Measurement of very low rates of cell proliferation by heavy water labeling of DNA and gas chromatography/pyrolysis/isotope ratio-mass spectrometric analysis. Nat Protoc 2007;2:3058-3062