Expression, purification, and characterization of pro-phenoloxidase-activating serine protease from: Spodoptera litura

Archives of Insect Biochemistry and Physiology

Volumn 72, Issue 2, 2009, Pages 61-73

  Arora, Naresh ^a   Hoque, M E ^a,b   Rajagopal, R ^a   Sachdev, Bindiya ^a   Bhatnagar, Raj K ^a  

^a INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY ICGEB   (India)

^b BANGLADESH AGRICULTURAL RESEARCH INSTITUTE   (Bangladesh)

Author keywords

Activation of phenoloxidase; Innate immunity; Melanization; Serine protease; Spodoptera litura

Indexed keywords

MONOPHENOL MONOOXYGENASE; PREPHENOLOXIDASE ACTIVATING ENZYME; PREPHENOLOXIDASE-ACTIVATING ENZYME; SERINE PROTEINASE;

AMINO ACID SEQUENCE; ANIMAL; ARMYWORM; ARTICLE; BIOSYNTHESIS; ENZYME ACTIVATION; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYLOGENY; SEQUENCE ALIGNMENT;

AMINO ACID SEQUENCE; ANIMALS; CLONING, MOLECULAR; ENZYME ACTIVATION; GENE EXPRESSION REGULATION, ENZYMOLOGIC; MOLECULAR SEQUENCE DATA; MONOPHENOL MONOOXYGENASE; PHYLOGENY; SEQUENCE ALIGNMENT; SERINE ENDOPEPTIDASES; SPODOPTERA;

SPODOPTERA LITURA;

EID: 70349622763     PISSN: 07394462     EISSN: 15206327     Source Type: Journal    
DOI: 10.1002/arch.20323     Document Type: Article

References (20)

1. Agrawal N, Adak T, Chauhan VS, Bhatnagar RK. 2008. Propeptide-mediated specific inhibition of a recombinant serine protease from Indian malarial vector, Anopheles culicifacies. Int J Pept Res Ther 14:21-28.
2. Ashida M, Brey PT. 1998. Chapter 6. Recent advances research on insect prophenoloxidase cascade. In: PT Brey, D Hultmark, editors. Molecular mechanisms of immune responses in insects. London: Chapman & Hall. p 135-172.
3. Ashida M, Kinoshita K, Brey PT. 1990. Studies on prophenol oxidase activation in the mosquito Aedes aegypti L. Eur J Biochem 188:507-515.
4. Bhatnagar RK, Arora N, Sachidanand S, Shahabuddin M, Keister D, Chauhan VS. 2003. Synthetic propeptide inhibits mosquito midgut chitinase and blocks sporognic development of malaria parasite. Biochem Biophys Res Commun 304:783-787.
5. Bradford M. 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254.
6. Cerenius L, Söderhäll K. 2004. The prophenoloxidase-activating system in invertebrates. Immunol Rev 198:116-126.
7. Chosa N, Fukumitsu T, Fujimoto K, Ohnishi E. 1997. Activation of prophenoloxidase A1 by an activating enzyme in Drosophila melanogaster. Insect Biochem Mol Biol 27:61-68.
8. Gillespie JP, Kanost MR, Trenczek T. 1997. Biological mediators of insect immunity. Annu Rev Entomol 42:611-643.
9. Hall M, Scott T, Sugumaran M, Soderhall K, Law JH. 1995. Proenzyme of Manduca sexta phenol oxidase: purification, activation, substrate specificity of the active enzyme, and molecular cloning. Proc Natl Acad Sci USA 92:7764-7768.
10. Ji C, Wang Y, Ross J, Jiang H. 2003. Expression and in vitro activation of Manduca sexta prophenoloxidase-activating proteinase-2 precursor (proPAP-2) from baculovirus-infected insect cells. Protein Expr Purif 29:235-243.
11. Jiang H, Wang Y, Ma C, Kanost MR. 1997. Subunit composition of pro-phenol oxidase from Manduca sexta: molecular cloning of subunit ProPO-P1. Insect Biochem Mol Biol 27:835-850.
12. Jiang H, Wang Y, Kanost MR. 1998. Pro-phenol oxidase activating proteinase from an insect, Manduca sexta: a bacteria-inducible protein similar to Drosophila easter. Proc Natl Acad Sci USA 95:12220-12225.
13. Kawabata T, Yasuhara Y, Ochiai M, Matsura S, Ashida M. 1995. Molecular cloning of insect prophenol oxidase: a copper containing protein homologous to protein arthropoid hemocyanin. Proc Natl Acad Sci USA 92:7774-7778.
14. Korde R, Bhardwaj A, Singh R, Srivastava A, Chauhan VS, Bhatnagar RK, Malhotra PM. 2008. A prodomain peptide of Plasmodium falciparum cysteine protease (Falcipain-2) inhibits malaria parasite development. J Med Chem 51:3116-3123.
15. Lee SY, Kwon TH, Hyun JH, Choi JS, Kawabata SI, Iwanaga S, Lee BL. 1998. In vitro activation of pro-phenol-oxidase by two kinds of pro-phenol-oxidase-activating factors isolated from hemolymph of coleopteran, Holotrichia diomphalia larvae. Eur J Biochem 254:50-57.
16. Rajagopal R, Thamilarasi K, Venkatesh GR, Srinivas P, Bhatnagar RK. 2005. Immune cascade of Spodoptera litura: cloning, expression and characterization of inducible prophenoloxidase. Biochem Biophys Res Commun 337:394-400.
17. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
18. Satoh D, Horii A, Ochiai M, Ashida M. 1999. Prophenoloxidase-activating enzyme of the silkworm, Bombyx mori: purification, characterisation and cDNA cloning. J Biol Chem 274:7441-7453.
19. Vilmos P, Kurucz E. 1998. Insect immunity: evolutionary roots of the mammalian innate immune system. Immunol Lett 62:59-66.
20. Wang Y, Jiang H. 2004. Prophenoloxidase (proPO) activation in Manduca sexta: an analysis of molecular interactions among proPO, proPO-activating proteinase-3, and a co-factor. Insect Biochem Mol Biol 34:731-742.