The cytosolic subunit p67phox of the NADPH-oxidase complex does not bind NADPH

FEBS Letters

Volumn 583, Issue 19, 2009, Pages 3225-3229

  Baciou, Laura ^a   Erard, Marie ^a   Dagher, Marie Claire ^b   Bizouarn, Tania ^a  

^a UNIVERSITÉ PARIS SUD   (France)

^b TIM C TheREx   (France)

Author keywords

NADPH binding; NADPH dialdehyde; NADPH oxidase; p67phox; Tryptophan fluorescence

Indexed keywords

PROTEIN P67; PROTEIN SUBUNIT; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE OXIDASE; SUPEROXIDE; TRYPTOPHAN; NEUTROPHIL CYTOSOL FACTOR 67K; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; PHOSPHOPROTEIN; RECOMBINANT PROTEIN;

ANIMAL CELL; ARTICLE; BINDING SITE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOSOL; FLUORESCENCE SPECTROSCOPY; HUMAN; HUMAN CELL; NEUTROPHIL; NONHUMAN; PHAGOCYTE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN LOCALIZATION; PROTEIN MODIFICATION; PROTEIN PROTEIN INTERACTION; ANIMAL; CATTLE; CELL MEMBRANE; COMPARATIVE STUDY; ENZYMOLOGY; GENETICS; METABOLISM;

ANIMALS; BINDING SITES; CATTLE; CELL MEMBRANE; CYTOSOL; HUMANS; NADP; NADPH OXIDASE; NEUTROPHILS; PHOSPHOPROTEINS; RECOMBINANT PROTEINS;

EID: 70349561328     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2009.09.011     Document Type: Article

References (31)

1. Cross A.R., and Segal A.W. The NADPH oxidase of professional phagocytes-prototype of the NOX electron transport chain systems. Biochim. Biophys. Acta 1657 (2004) 1-22
2. Quinn M.T., and Gauss K.A. Structure and regulation of the neutrophil respiratory burst oxidase: comparison with nonphagocyte oxidases. J. Leukoc. Biol. 76 (2004) 760-781
3. Vignais P.V. The superoxide-generating NADPH oxidase: structural aspects and activation mechanism. Cell. Mol. Life Sci. 59 (2002) 1428-1459
4. Dagher M.C., and Pick E. Opening the black box: lessons from cell-free systems on the phagocyte NADPH-oxidase. Biochimie 89 (2007) 1123-1132
5. Freeman J.L., and Lambeth J.D. NADPH oxidase activity is independent of p47phox in vitro. J. Biol. Chem. 271 (1996) 22578-22582
6. Umei T., Takeshige K., and Minakami S. NADPH-binding component of the superoxide-generating oxidase in unstimulated neutrophils and the neutrophils from the patients with chronic granulomatous disease. Biochem. J. 243 (1987) 467-472
7. Sha'ag D., and Pick E. Macrophage-derived superoxide-generating NADPH oxidase in an amphiphile-activated, cell-free system; partial purification of the cytosolic component and evidence that it may contain the NADPH binding site. Biochim. Biophys. Acta 952 (1988) 213-219
8. Umei T., Babior B.M., Curnutte J.T., and Smith R.M. Identification of the NADPH-binding subunit of the respiratory burst oxidase. J. Biol. Chem. 266 (1991) 6019-6022
9. Ge F., and Guillory R.J. NADPH-binding protein of the neutrophil superoxide-generating oxidase of guinea pigs. Proc. Natl. Acad. Sci. USA 91 (1994) 8622-8626
10. Smith R.M., Curnutte J.T., and Babior B.M. Affinity labeling of the cytosolic and membrane components of the respiratory burst oxidase by the 2′,3′-dialdehyde derivative of NADPH. Evidence for a cytosolic location of the nucleotide-binding site in the resting cell. J. Biol. Chem. 264 (1989) 1958-1962
11. Segal A.W., et al. Cytochrome b-245 is a flavocytochrome containing FAD and the NADPH-binding site of the microbicidal oxidase of phagocytes. Biochem. J. 284 Pt 3 (1992) 781-788
12. Ravel P., and Lederer F. Affinity-labeling of an NADPH-binding site on the heavy subunit of flavocytochrome b558 in particulate NADPH oxidase from activated human neutrophils. Biochem. Biophys. Res. Commun. 196 (1993) 543-552
13. Doussière J., Brandolin G., Derrien V., and Vignais P.V. Critical assessment of the presence of an NADPH binding site on neutrophil cytochrome b558 by photoaffinity and immunochemical labeling. Biochemistry 32 (1993) 8880-8887
14. Koshkin V., and Pick E. Generation of superoxide by purified and relipidated cytochrome b559 in the absence of cytosolic activators. FEBS Lett. 327 (1993) 57-62
15. Dang P.M., Johnson J.L., and Babior B.M. Binding of nicotinamide adenine dinucleotide phosphate to the tetratricopeptide repeat domains at the N-terminus of p67PHOX, a subunit of the leukocyte nicotinamide adenine dinucleotide phosphate oxidase. Biochemistry 39 (2000) 3069-3075
16. Sheppard F.R., Kelher M.R., Moore E.E., McLaughlin N.J., Banerjee A., and Silliman C.C. Structural organization of the neutrophil NADPH oxidase: phosphorylation and translocation during priming and activation. J. Leukoc. Biol. 78 (2005) 1025-1042
17. Groemping Y., and Rittinger K. Activation and assembly of the NADPH oxidase: a structural perspective. Biochem. J. 386 (2005) 401-416
18. Bizouarn T., Diggle C., Quirk P.G., Grimley R.L., Cotton N.P., Thomas C.M., and Jackson J.B. Interaction of nucleotides with the NAD(H)-binding domain of the proton-translocating transhydrogenase of Rhodospirillum rubrum. J. Biol. Chem. 271 (1996) 10103-10108
19. Hu X., Zhang J., Fjellstrom O., Bizouarn T., and Rydstrom J. Site-directed mutagenesis of charged and potentially proton-carrying residues in the beta subunit of the proton-translocating nicotinamide nucleotide transhydrogenase from Escherichia coli. Characterization of the beta H91, beta D392, and beta K424 mutants. Biochemistry 38 (1999) 1652-1658
20. Morel F., Doussiere J., Stasia M.J., and Vignais P.V. The respiratory burst of bovine neutrophils. Role of a b type cytochrome and coenzyme specificity. Eur. J. Biochem. 152 (1985) 669-679
21. Li B., and Lin S.X. Fluorescence-energy transfer in human estradiol 17 beta-dehydrogenase-NADPH complex and studies on the coenzyme binding. Eur. J. Biochem. 235 (1996) 180-186
22. Lark R.H., and Colman R.F. Reaction of the 2′,3′-dialdehyde derivative of NADPH at a nucleotide site of bovine liver glutamate dehydrogenase. J. Biol. Chem. 261 (1986) 10659-10666
23. van Gelder B., and Slater E.C. The extinction coefficient of cytochrome c. Biochim. Biophys. Acta 58 (1962) 593-595
24. -{radical dot}-generating oxidase from bovine polymorphonuclear neutrophils. Biochemistry 24 (1985) 7231-7239
25. Bunger P.L., Swain S.D., Clements M.K., Siemsen D.W., Davis A.R., Gauss K.A., and Quinn M.T. Cloning and expression of bovine p47-phox and p67-phox: comparison with the human and murine homologs. J. Leukoc. Biol. 67 (2000) 63-72
26. Babior B.M. The respiratory burst of phagocytes. J. Clin. Invest. 73 (1984) 599-601
27. Umei T., Takeshige K., and Minakami S. NADPH binding component of neutrophil superoxide-generating oxidase. J. Biol. Chem. 261 (1986) 5229-5232
28. Smith R.M., Connor J.A., Chen L.M., and Babior B.M. The cytosolic subunit p67phox contains an NADPH-binding site that participates in catalysis by the leukocyte NADPH oxidase. J. Clin. Invest. 98 (1996) 977-983
29. Dang P.M., Babior B.M., and Smith R.M. NADPH dehydrogenase activity of p67PHOX, a cytosolic subunit of the leukocyte NADPH oxidase. Biochemistry 38 (1999) 5746-5753
30. Taylor W.R., Jones D.T., and Segal A.W. A structural model for the nucleotide binding domains of the flavocytochrome b-245 beta-chain. Protein Sci. 2 (1993) 1675-1685
31. Lapouge K., Smith S.J., Walker P.A., Gamblin S.J., Smerdon S.J., and Rittinger K. Structure of the TPR domain of p67phox in complex with Rac.GTP. Mol. Cell 6 (2000) 899-907