Escherichia coli expression and in vitro activation of a unique ligninolytic peroxidase that has a catalytic tyrosine residue

Protein Expression and Purification

Volumn 68, Issue 2, 2009, Pages 208-214

  Miki, Yuta ^a   Morales, María ^a   Ruiz Dueñas, Francisco J ^a   Martínez, María Jesús ^a   Wariishi, Hiroyuki ^b   Martínez, Angel T ^a  

^a CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

^b KYUSHU UNIVERSITY   (Japan)

Author keywords

Escherichia coli expression; Heme protein; In vitro activation; Lignin peroxidase; Trametes cervina; Veratryl alcohol

Indexed keywords

BENZYL ALCOHOL DERIVATIVE; HYDROGEN PEROXIDE; LIGNIN PEROXIDASE; PEROXIDASE; TYROSINE; VERATRYL ALCOHOL;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ENZYME ACTIVATION; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; OXIDATION REDUCTION REACTION; PH; PROTEIN FOLDING; PROTEIN STABILITY; TRAMETES;

AMINO ACID SEQUENCE; BENZYL ALCOHOLS; CLONING, MOLECULAR; ENZYME ACTIVATION; ESCHERICHIA COLI; HYDROGEN PEROXIDE; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR SEQUENCE DATA; OXIDATION-REDUCTION; PEROXIDASES; PROTEIN FOLDING; PROTEIN STABILITY; TRAMETES; TYROSINE;

BASIDIOMYCOTA; ESCHERICHIA COLI; TRAMETES; TRAMETES CERVINA;

EID: 70349466798     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.06.003     Document Type: Article

References (37)

1. Gellerstedt G., and Henriksson G. Lignins: major sources, structure and properties. In: Belgacem M., and Gandini A. (Eds). Monomers, Polymers and Composites from Renewable Resources (2008), Elsevier, Amsterdam 201-224
2. Glasser W.G., Northey R.A., and Schultz T.P. Lignin: Historical, Biological, and Materials Perspectives (2000), American Chemical Society
3. Tien M., and Kirk T.K. Lignin-degrading enzyme from the hymenomycete Phanerochaete chrysosporium Burds. Science 221 (1983) 661-663
4. 2-requiring enzyme preparation involved in lignin biodegradation by the white rot basidiomycete Phanerochaete chrysosporium. Biochem. Biophys. Res. Commun. 114 (1983) 1077-1083
5. 2-requiring oxygenase. Proc. Natl. Acad. Sci. USA 81 (1984) 2280-2284
6. Hammel K.E., and Cullen D. Role of fungal peroxidases in biological ligninolysis. Curr. Opin. Plant Biol. 11 (2008) 349-355
7. Ruiz-Dueñas F.J., and Martínez A.T. Microbial degradation of lignin: how a bulky recalcitrant polymer is efficiently recycled in nature and how we can take advantage of this. Microb. Biotechnol. 2 (2009) 164-177
8. Kersten P.J., Kalyanaraman B., Hammel K.E., Reinhammar B., and Kirk T.K. Comparison of lignin peroxidase, horseradish peroxidase and laccase in the oxidation of methoxybenzenes. Biochem. J. 268 (1990) 475-480
9. Wariishi H., Sheng D.W., and Gold M.H. Oxidation of ferrocytochrome c by lignin peroxidase. Biochemistry 33 (1994) 5545-5552
10. Kirk T.K., and Farrell R.L. Enzymatic "combustion": the microbial degradation of lignin. Annu. Rev. Microbiol. 41 (1987) 465-505
11. Hammel K.E., Jensen K.A., Mozuch M.D., Landucci L.L., Tien M., and Pease E.A. Ligninolysis by a purified lignin peroxidase. J. Biol. Chem. 268 (1993) 12274-12281
12. A.T. Martínez, F.J. Ruiz-Dueñas, M.J. Martínez, J.C. del Río, A. Gutiérrez, Enzymatic delignification of plant cell-wall: from nature to mill, Curr. Opin. Biotechnol. (2009), doi: 10.1016/j.copbio.2009.05.002.
13. Mester T., Ambert-Balay K., Ciofi-Baffoni S., Banci L., Jones A.D., and Tien M. Oxidation of a tetrameric nonphenolic lignin model compound by lignin peroxidase. J. Biol. Chem. 276 (2001) 22985-22990
14. Doyle W.A., Blodig W., Veitch N.C., Piontek K., and Smith A.T. Two substrate interaction sites in lignin peroxidase revealed by site-directed mutagenesis. Biochemistry 37 (1998) 15097-15105
15. Blodig W., Smith A.T., Doyle W.A., and Piontek K. Crystal structures of pristine and oxidatively processed lignin peroxidase expressed in Escherichia coli and of the W171F variant that eliminates the redox active tryptophan 171. Implications for the reaction mechanism. J. Mol. Biol. 305 (2001) 851-861
16. Gelpke M.D.S., Lee J., and Gold M.H. Lignin peroxidase oxidation of veratryl alcohol: effects of the mutants H82A, Q222A, W171A, and F267L. Biochemistry 41 (2002) 3498-3506
17. Johjima T., Wariishi H., and Tanaka H. Veratryl alcohol binding sites of lignin peroxidase from Phanerochaete chrysosporium. J. Mol. Catal. B: Enzym. 17 (2002) 49-57
18. Pérez-Boada M., Ruiz-Dueñas F.J., Pogni R., Basosi R., Choinowski T., Martínez M.J., Piontek K., and Martínez A.T. Versatile peroxidase oxidation of high redox potential aromatic compounds: site-directed mutagenesis, spectroscopic and crystallographic investigations of three long-range electron transfer pathways. J. Mol. Biol. 354 (2005) 385-402
19. Ruiz-Dueñas F.J., Morales M., Mate M.J., Romero A., Martínez M.J., Smith A.T., and Martínez A.T. Site-directed mutagenesis of the catalytic tryptophan environment in Pleurotus eryngii versatile peroxidase. Biochemistry 47 (2008) 1685-1695
20. Tsukihara T., Honda Y., Sakai R., Watanabe T., and Watanabe T. Mechanism for oxidation of high-molecular-weight substrates by a fungal versatile peroxidase, MnP2. Appl. Environ. Microbiol. 74 (2008) 2873-2881
21. Martínez A.T. Molecular biology and structure-function of lignin-degrading heme peroxidases. Enzyme Microb. Technol. 30 (2002) 425-444
22. Ruiz-Dueñas F.J., Morales M., García E., Miki Y., Martínez M.J., and Martínez A.T. Substrate oxidation sites in versatile peroxidase and other basidiomycete peroxidases. J. Exp. Bot. 60 (2009) 441-452
23. Miki Y., Tanaka H., Nakamura M., and Wariishi H. Isolation and characterization of a novel lignin peroxidase from the white-rot basidiomycete Trametes cervina. J. Fac. Agr. Kyushu Univ. 51 (2006) 99-104
24. Y. Miki, H. Wariishi, H. Tanaka, Lignin peroxidase from the white-rot basidiomycete Trametes cervina, in: Proceedings of the 12th ISWPC, vol. II, Madison, 9-12 June, 2003, p. 95.
25. Stubbe J., and Der Donk W.A. Protein radicals in enzyme catalysis. Chem. Rev. 98 (1998) 705-762
26. Ruiz-Dueñas F.J., Pogni R., Morales M., Giansanti S., Mate M.J., Romero A., Martínez M.J., Basosi R., and Martínez A.T. Protein radicals in fungal versatile peroxidase: catalytic tryptophan radical in both compound I and compound II and studies on W164Y, W164H and W164S variants. J. Biol. Chem. 284 (2009) 7986-7994
27. Y. Miki, H. Wariishi, Molecular characterization of a novel lignin peroxidase from the white-rot basidiomycete Trametes cervina, in: Proceedings of the International Symposium on Mushroom Science, Akita, Japan, 20-22 September, 2006, pp. 183-184.
28. 2+ and heme. J. Biol. Chem. 265 (1990) 13335-13343
29. Whitwam R., and Tien M. Heterologous expression and reconstitution of fungal Mn peroxidase. Arch. Biochem. Biophys. 333 (1996) 439-446
30. 2+ and haem. Biochem. J. 315 (1996) 15-19
31. Pérez-Boada M., Doyle W.A., Ruiz-Dueñas F.J., Martínez M.J., Martínez A.T., and Smith A.T. Expression of Pleurotus eryngii versatile peroxidase in Escherichia coli and optimisation of in vitro folding. Enzyme Microb. Technol. 30 (2002) 518-524
32. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
33. Oyadomari M., Shinohara H., Johjima T., Wariishi H., and Tanaka H. Electrochemical characterization of lignin peroxidase from the white-rot basidiomycete Phanerochaete chrysosporium. J. Mol. Catal. B: Enzym. 21 (2003) 291-297
34. Zeng J., and Fenna R.E. X-ray crystal structure of canine myeloperoxidase at 3 Å resolution. J. Mol. Biol. 226 (2009) 185-207
35. Carpena X., Loprasert S., Mongkolsuk S., Switala J., Loewen P.C., and Fita I. Catalase-peroxidase KatG of Burkholderia pseudomallei at 1.7 Å resolution. J. Mol. Biol. 327 (2009) 475-489
36. Shimada M., Nakatsubo F., Kirk T.K., and Higuchi T. Biosynthesis of the secondary metabolite veratryl alcohol in relation to lignin degradation by Phanerochaete chrysosporium. Arch. Microbiol. 129 (1981) 321-324
37. 2 production by Phanerochaete chrysosporium. J. Bacteriol. 169 (1987) 2195-2201