Fgf8 morphogen gradient forms by a source-sink mechanism with freely diffusing molecules

Nature

Volumn 461, Issue 7263, 2009, Pages 533-536

  Yu, Shuizi Rachel ^a,b   Burkhardt, Markus ^a,b   Nowak, Matthias ^a,b   Ries, Jonas ^a,b   Petráek, Zdenk ^a,b   Scholpp, Steffen ^a   Schwille, Petra ^a,b   Brand, Michael ^a,b  

^a DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^b CENTER FOR REGENERATIVE THERAPIES DRESDEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

FIBROBLAST GROWTH FACTOR 8; MORPHOGEN;

CONCENTRATION (COMPOSITION); CYTOLOGY; EMBRYO; EXPERIMENTAL STUDY; FISH; GENE EXPRESSION; GRADIENT ANALYSIS; MOLECULAR ANALYSIS; MORPHOGENESIS; SOURCE-SINK DYNAMICS;

ARTICLE; CONCENTRATION (PARAMETERS); DIFFUSION; EMBRYO; ENDOCYTOSIS; EXTRACELLULAR SPACE; FLUORESCENCE CORRELATION SPECTROSCOPY; MOLECULE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; SOURCE SINK RELATIONSHIP; TISSUE DIFFERENTIATION; ZEBRA FISH;

ANIMALS; DIFFUSION; EMBRYO, NONMAMMALIAN; ENDOCYTOSIS; EXTRACELLULAR SPACE; FIBROBLAST GROWTH FACTORS; GASTRULATION; GREEN FLUORESCENT PROTEINS; MODELS, BIOLOGICAL; MORPHOGENESIS; RECEPTORS, FIBROBLAST GROWTH FACTOR; ZEBRAFISH; ZEBRAFISH PROTEINS;

DANIO RERIO;

EID: 70349453919     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature08391     Document Type: Article

References (32)

1. Wolpert, L. Positional information and the spatial pattern of cellular differentiation. J. Theor. Biol. 25, 1-47 (1969).
2. Tabata, T. & Takei, Y. Morphogens, their identification and regulation. Development 131, 703-712 (2004).
3. Crick, F. Diffusion in embryogenesis. Nature 225, 420-422 (1970).
4. Kerszberg, M. & Wolpert, L. Mechanisms for positional signalling by morphogen transport: a theoretical study. J. Theor. Biol. 191, 103-114 (1998).
5. Entchev, E. V., Schwabedissen, A. & Gonzalez-Gaitan, M. Gradient formation of the TGF-b homolog Dpp. Cell 103, 981-992 (2000).
6. Strigini, M. & Cohen, S. M. Wingless gradient formation in the Drosophila wing. Curr. Biol. 10, 293-300 (2000).
7. McDowell, N., Gurdon, J. B. & Grainger, D. J. Formation of a functional morphogen gradient by a passive process in tissue from the early Xenopus embryo. Int. J. Dev. Biol. 45, 199-207 (2001).
8. Gregor, T., Bialek, W., de Ruyter van Steveninck, R. R., Tank, D. W. & Wieschaus, E. F. Diffusion and scaling during early embryonic pattern formation. Proc. Natl Acad. Sci. USA 102, 18403-18407 (2005).
9. Lander, A. D. Morpheus unbound: reimagining the morphogen gradient. Cell 128, 245-256 (2007).
10. Kicheva, A. et al. Kinetics of morphogen gradient formation. Science 315, 521-525 (2007).
11. Gregor, T., Wieschaus, E. F., McGregor, A. P., Bialek, W. & Tank, D. W. Stability and nuclear dynamics of the bicoid morphogen gradient. Cell 130, 141-152 (2007).
12. Boldajipour, B. et al. Control of chemokine-guided cell migration by ligand sequestration. Cell 132, 463-473 (2008).
13. Reifers, F. et al. Fgf8 is mutated in zebrafish acerebellar (ace) mutants and is required for maintenance of midbrain-hindbrain boundary development and somitogenesis. Development 125, 2381-2395 (1998).
14. Reifers, F., Walsh, E. C., Leger, S., Stainier, D. Y. & Brand, M. Induction and differentiation of the zebrafish heart requires fibroblast growth factor 8 (fgf8/ acerebellar). Development 127, 225-235 (2000).
15. Goldfarb, M. Functions of fibroblast growth factors in vertebrate development. Cytokine Growth Factor Rev. 7, 311-325 (1996).
16. Fürthauer, M., Reifers, F., Brand, M., Thisse, B. & Thisse, C. Sprouty4 acts in vivo as a feedback-induced antagonist of FGF signalling in zebrafish. Development 128, 2175-2186 (2001).
17. Raible, F. & Brand, M. Tight transcriptional control of the ETS domain factors Erm and Pea3 by Fgf signalling during early zebrafish nervous system development. Mech. Dev. 107, 105-117 (2001).
18. Scholpp, S. & Brand, M. Endocytosis controls spreading and effective signalling range of Fgf8 protein. Curr. Biol. 14, 1834-1841 (2004).
19. Rigler, R., Mets, U., Widengren, J. & Kask, P. Fluorescence correlation spectroscopy with high count rate and low-background: analysis of translational diffusion. Eur. Biophys. J. 22, 169-175 (1993).
20. Bacia, K., Kim, S. A. & Schwille, P. Fluorescence cross-correlation spectroscopy in living cells. Nature Methods 3, 83-89 (2006).
21. Amaya, E., Musci, T. J. & Kirschner, M. W. Expression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos. Cell 66, 257-270 (1991).
22. Ries, J., Yu, S. R., Burkhardt, M., Brand, M. & Schwille, P. Modular scanning FCS quantifies receptor-ligand interactions in living multicellular organisms. Nature Methods. doi:10.1038/nmeth.1355 (2 August 2009).
23. Petrás?ek, Z. & Schwille, P. Precise measurement of diffusion coefficients using scanning fluorescence correlation spectroscopy. Biophys. J. 94, 1437-1448 (2008).
24. Brinkmeier, M., Dörre, K., Stephan, J. & Eigen, M. Two beam cross-correlation: a method to characterize transport phenomena in micrometer-sized structures. Anal. Chem. 71, 609-616 (1999).
25. Dertinger, T. et al. Two-focus fluorescence correlation spectroscopy: a new tool for accurate and absolute diffusion measurements. ChemPhysChem 8, 433-443 (2007).
26. Hou, S., Maccarana, M., Min, T. H., Strate, I. & Pera, E. M. The secreted serine protease xHtrA1 stimulates long-range FGF signalling in the early Xenopus embryo. Dev. Cell 13, 226-241 (2007).
27. Baeg, G. H., Selva, E. M., Goodman, R. M., Dasgupta, R. & Perrimon, N. The Wingless morphogen gradient is established by the cooperative action of Frizzled and Heparan Sulphate Proteoglycan receptors. Dev. Biol. 276, 89-100 (2004).
28. Desai, U. R., Wang, H. & Linhardt, R. J. Substrate specificity of the heparin lyases from Flavobacterium heparinum. Arch. Biochem. Biophys. 306, 461-468 (1993).
29. Robinson, M. S., Watts, C. & Zerial, M. Membrane dynamics in endocytosis. Cell 84, 13-21 (1996).
30. Westerfield, M. The Zebrafish Book; a Guide for the Laboratory use of Zebrafish (Danio Rerio) (Univ. of Oregon Press, 2000).
31. Petrov, E. P., Ohrt, T., Winkler, R. G. & Schwille, P. Diffusion and segmental dynamics of double-stranded DNA. Phys. Rev. Lett. 97, 258101 (2006).
32. Burkhardt, M. & Schwille, P. Electron multiplying CCD based detection for spatially resolved fluorescence correlation spectroscopy. Opt. Express 14, 5013-5020 (2006).