Nitrogen regulates chitinase gene expression in a marine bacterium

ISME Journal

Volumn 3, Issue 9, 2009, Pages 1064-1069

  Delpin, Marina W ^a,b   Goodman, Amanda E ^a  

^a FLINDERS UNIVERSITY   (Australia)

^b TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

5'RACE; Chitinase; Marine bacterium; Nitrogen; Pseudoalteromonas; Regulation

Indexed keywords

BETA GALACTOSIDASE; CHITINASE; GLUTAMIC ACID; N ACETYLGLUCOSAMINE; NITROGEN; QUATERNARY AMMONIUM DERIVATIVE;

ARTICLE; BIOSYNTHESIS; GENE EXPRESSION REGULATION; GENE FUSION; GENETICS; METABOLISM; PHYSIOLOGY; PSEUDOALTEROMONAS; REPORTER GENE; TRANSCRIPTION INITIATION SITE;

ACETYLGLUCOSAMINE; ARTIFICIAL GENE FUSION; BETA-GALACTOSIDASE; CHITINASE; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REPORTER; GLUTAMIC ACID; NITROGEN; PSEUDOALTEROMONAS; QUATERNARY AMMONIUM COMPOUNDS; TRANSCRIPTION INITIATION SITE;

BACTERIA (MICROORGANISMS); PSEUDOALTEROMONAS; PSEUDOALTEROMONAS SP.;

EID: 70349446433     PISSN: 17517362     EISSN: 17517370     Source Type: Journal    
DOI: 10.1038/ismej.2009.49     Document Type: Article

References (23)

1. Albertson NH, Stretton S, Pongpattanakitshote S, Östling J, Marshall KC, Goodman AE et al. (1996). Construction and use of a new vector/transposon, pLBT::mini-Tn10:lac:kan, to identify environmentally responsive genes in a marine bacterium. FEMS Microbiol Lett 140: 287-294
2. Atkinson MR, Blauwkamp TA, Bondarenko V, Studitsky V, Ninfa AJ. (2002). Activation of the glnA, glnK and nac promoters as Escherichia coli undergoes the transition from nitrogen excess growth to nitrogen starvation. J Bacteriol 184: 5358-5363
3. Buck M, Miller S, Drummond M, Dixon R. (1986). Upstream activator sequences are present in the promoters of nitrogen fixation genes. Nature 320: 374-378
4. Cottrell MT, Kirchman DL. (2000). Natural assemblages of marine proteobacteria and members of the Cytophaga- Flavobacter cluster consuming low- and high-molecular- weight dissolved organic matter. Appl Environ Microbiol 66: 1692-1697
5. Davalos M, Fourment J, Lucas A, Bergès H, Kahn D. (2004). Nitrogen regulation in Sinorhizobium meliloti probed with whole genome arrays. FEMS Microbiol Lett 241: 33-40
6. Delpin MW, Goodman AE. (2009). Nutrient regime regulates complex transcriptional start site usage within a Pseudoalteromonas chitinase gene cluster. ISME J (in press
7. Gooday GW. (1990). The ecology of chitin degradation. In: Marshall KC (ed). Advances in Microbial Ecology, vol.11, Plenum Press: New York. pp 387-430
8. Huang X, Miller W. (1991). A time-efficient, linear-space local similarity algorithm. Adv Appl Math 12: 337-357
9. Keyhani NO, Roseman S. (1999). Physiological aspects of chitin catabolism in marine bacteria. Biochim Biophys Acta 1473: 108-122
10. Magasanik B. (1996). Regulation of nitrogen utilization. In: Neidhardt FC (ed). Escherichia coli and Salmonella Cellular and Molecular Biology, vol 1, ASM Press: Washington. pp 1344-1356
11. McGowan CC, Necheva AS, Forsyth MH, Cover TL, Blaser MJ. (2003). Promoter analysis of Helicobacter pylori genes with enhanced expression at low pH. Mol Microbiol 48: 1225-1239
12. Miller JH. (1972). Experiments in Molecular Genetics. Cold Spring Harbor: New York
13. Östling J, Goodman AE, Kjelleberg S. (1991). Behaviour of InP-1 plasmids and a miniMu transposon in a marine Vibrio sp. S14: isolation of starvation inducible lac operon fusions. FEMS Microb Ecol 86: 83-94
14. Reitzer LJ, Magasanik B. (1986). Transcription of glnA in Escherichia coli is stimulated by an activator bound to sites far from the promoter. Cell 45: 785-792
15. Reitzer L, Schneider BL. (2001). Metabolic context and possible physiological themes of s54-dependent genes in Escherichia coli. Microbiol Mol Biol R 65: 422-444
16. Schneider BL, Shiau S-P, Reitzer LJ. (1991). Role of multiple environmental stimuli in control of transcription from a nitrogen-regulated promoter in Escherichia coli with weak or no activator-binding sites. J Bacteriol 173: 6355-6363
17. Stretton S, Marshall KC, Dawes IW, Goodman AE. (1996). Characterisation of carbon dioxide-inducible genes of the marine bacterium, Pseudomonas sp. S91. FEMS Microbiol Lett 140: 37-42
18. Techkarnjanaruk S, Pongpattanakitshote S, Goodman AE. (1997). Use of a promoterless lacZ gene insertion to investigate chitinase gene expression in the marine bacterium Pseudoalteromonas sp. S9. Appl Environ Microbiol 63: 2989-2996
19. Tillett D, Burns BP, Neilan BA. (2000). Optimized rapid amplification of cDNA ends (RACE) for mapping bacterial mRNA transcripts. Biotechniques 28: 448-456
20. Wetzel RG. (1991). Extracellular enzymatic interactions: storage, redistribution, and interspecific communication. In: Chrost RJ (ed). Microbial Enzymes in Aquatic Environments. Springer-Verlag: New York. pp 6-26
21. Zar JH. (1996). Biostatistical Analysis. Prentice-Hall: New Jersey
22. Zehr JP, Ward BB. (2002). Nitrogen cycling in the ocean: new perspectives on processes and paradigms. Appl Environ Microbiol 68: 1015-1024
23. Zimmer DP, Soupene E, Lee HL, Wendisch VF, Khodursky AB, Peter BJ et al. (2000). Nitrogen regulatory protein C-controlled genes of Escherichia coli: scavenging as a defense against nitrogen limitation. Proc Natl Acad Sci USA 97: 14674-14679