Pronuclear DNA injection for the production of transgenic rats

Methods in Molecular Biology

Volumn 561, Issue , 2009, Pages 73-88

  Cozzi, Jean ^a   Anegon, Ignacio ^b   Braun, Valérie ^a   Gross, Anne Catherine ^a   Merrouche, Christel ^a   Cherifi, Yacine ^a  

^a genOway   (France)

^b INSERM   (France)

Author keywords

Embryo transfer; Isoflurane; Microinjection; Ovarian cycle synchronization; Sprague Dawley; Transgenic rat

Indexed keywords

RATTUS;

DNA;

ANIMAL; ANIMAL DISEASE; ANIMAL EMBRYO; ARTICLE; CELL NUCLEUS; CYTOLOGY; EMBRYO TRANSFER; FEMALE; GENE TRANSFER; GENETICS; MALE; METHODOLOGY; MICROINJECTION; PHYSIOLOGY; PRENATAL DEVELOPMENT; RAT; SPRAGUE DAWLEY RAT; TRANSGENE; TRANSGENIC ANIMAL; ZYGOTE;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; CELL NUCLEUS; DNA; EMBRYO TRANSFER; EMBRYO, MAMMALIAN; FEMALE; GENE TRANSFER TECHNIQUES; MALE; MICROINJECTIONS; RATS; RATS, SPRAGUE-DAWLEY; TRANSGENES; ZYGOTE;

EID: 70349229624     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-019-9_5     Document Type: Article

References (9)

1. Cozzi, J., Fraichard, A. and Thiam, K. (2008) Use of genetically modified rats for translational medicine. Drug Discov Today, 13 (11-12), 488-494.
2. Mullins, J.J., Peters, J. and Ganten, D. (1990) Fulminant hypertension in transgenic rats harbouring the mouse Ren-2 gene. Nature, 344, 541-544.
3. Hammer, R.E., Maika, S.D., Richardson, J.A., Tang, J.P. and Taurog, J.D. (1990) Spontaneous inflammatory disease in transgenic rats expressing HLA-B27 and human beta 2m: An animal model of HLA-B27-associated human disorders. Cell, 63, 1099-1112.
4. Armstrong, D.T. and Opavsky, M.A. (1988) Superovulation of immature rats by continuous infusion of follicle-stimulating hormone. Biol Reprod, 39, 511-518.
5. Miyoshi, K., Funahashi, H., Okuda, K. and Niwa, K. (1994) Development of rat one-cell embryos in a chemically defined medium: Effects of glucose, phosphate and osmolarity. J Reprod Fertil, 100, 21-26.
6. Vickery, B.H. and McRae, G.I. (1980) Synchronization of oestrus in adult female rats by utilizing the paradoxical effects of an LH-RH agonist. J Reprod Fertil, 60, 399-402.
7. Filipiak, W.E. and Saunders, T.L. (2006) Advances in transgenic rat production. Transgenic Res, 15, 673-686.
8. Hamilton, G.S. and Armstrong, D.T. (1991) The superovulation of synchronous adult rats using follicle-stimulating hormone delivered by continuous infusion. Biol Reprod, 44, 851-856.
9. Smith, J.C., Corbin, T.J., McCabe, J.G. and Bolon, B. (2004) Isoflurane with morphine is a suitable anaesthetic regimen for embryo transfer in the production of transgenic rats. Lab Anim, 38, 38-43.