Allergen content of grass pollen preparations for skin prick testing and sublingual immunotherapy

Allergy: European Journal of Allergy and Clinical Immunology

Volumn 64, Issue 10, 2009, Pages 1486-1492

  Sander, I ^a   Fleischer, C ^a   Meurer, U ^a   Bruning T ^a   Raulf Heimsoth, M ^a  

^a RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

Allergen concentration; Grass pollen allergy; Phleum pratense; Protein concentration; Skin prick test diagnostics

Indexed keywords

ALLERSLIT; GRASS POLLEN VACCINE; IGEVAC; IMMUNOGLOBULIN E; PHL P 5A; POLLEN ANTIGEN; RECOMBINANT PROTEIN; SLITONE; STALORAL; SUBLIVAC; SULGEN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENZYME IMMUNOASSAY; GRASS POLLEN; HUMAN; IMMUNOBLOTTING; IMMUNOTHERAPY; MAINTENANCE DRUG DOSE; PH; PHLEUM PRATENSE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRICK TEST; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONTENT; QUANTITATIVE ANALYSIS;

ADMINISTRATION, CUTANEOUS; ADMINISTRATION, SUBLINGUAL; ALLERGENS; DESENSITIZATION, IMMUNOLOGIC; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; IMMUNOBLOTTING; PLANT PROTEINS; POACEAE; POLLEN; RHINITIS, ALLERGIC, SEASONAL; SKIN TESTS;

EID: 70349216591     PISSN: 01054538     EISSN: 13989995     Source Type: Journal    
DOI: 10.1111/j.1398-9995.2009.02040.x     Document Type: Article

References (15)

1. Larenas-Linnemann D, Cox LS. European allergen extract units and potency: review of available information. Ann Allergy Asthma Immunol 2008 100 : 137 145.
2. van Ree R, Chapman MD, Ferreira F, Vieths S, Bryan D, Cromwell O et al. The CREATE project: development of certified reference materials for allergenic products and validation of methods for their quantification. Allergy 2008 63 : 310 326.
3. Becker WM, van Ree R, Fiebig H, Cromwell O, Weber B, Monsalve R et al. Validation of quantitative, allergen-specific ELISAs. Arb Paul Ehrlich Inst Bundesamt Sera Impfstoffe Frankf a M 2006 91 97.
4. Ramirez J, Obispo TM, Duffort D, Carpizo JA, Chamorro MJ, Barber D et al. Group 5 determination in Pooideae grass pollen extracts by monoclonal antibody-based ELISA. Correlation with biologic activity. Allergy 1997 52 : 806 813.
5. Duffort O, Quintana J, Ipsen H, Barber D, Polo F. Antigenic similarity among group 1 allergens from grasses and quantitation ELISA using monoclonal antibodies to Phl p 1. Int Arch Allergy Immunol 2008 145 : 283 290.
6. Meno K, Bøg A, Giselsson A, Grosch K, Hansen G, Jimeno L et al. Are there differences in the contents of grass skin prick test products from different manufactures that could influence diagnosis? Allergy 2007 62 : 49.
7. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970 227 : 680 685.
8. Blum H, Beier H, Gross HJ. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 1987 8 : 93 99.
9. Sander I, Flagge A, Merget R, Halder TM, Meyer HE, Baur X. Identification of wheat flour allergens by means of two-dimensional immunoblotting. J Allergy Clin Immunol 2001 107 : 907 913.
10. Wheeler AW, Jessberger B, Drachenberg KJ, Rakoski J. Design of an optimally-diagnostic skin test solution for diagnosis of sensitivity to timothy grass (Phleum pratense) pollen. Clin Exp Allergy 1996 26 : 897 902.
11. Focke M, Marth K, Flicker S, Valenta R. Heterogeneity of commercial timothy grass pollen extracts. Clin Exp Allergy 2008 38 : 1400 1408.
12. Bufe A, Ziegler-Kirbach E, Stoeckmann E, Heidemann P, Gehlhar K, Holland-Letz T et al. Efficacy of sublingual swallow immunotherapy in children with severe grass pollen allergic symptoms: a double-blind placebo-controlled study. Allergy 2004 59 : 498 504.
13. Hrabina M, Peltre G, van Ree R, Moingeon P. Grass pollen allergens. Clin Exp Allergy Reviews 2008 8 : 7 11.
14. Niederberger V, Laffer S, Froschl R, Kraft D, Rumpold H, Kapiotis S et al. IgE antibodies to recombinant pollen allergens (Phl p 1, Phl p 2, Phl p 5, and Bet v 2) account for a high percentage of grass pollen-specific IgE. J Allergy Clin Immunol 1998 101 : 258 264.
15. Mari A. Skin test with a timothy grass (Phleum pratense) pollen extract vs. IgE to a timothy extract vs. IgE to rPhl p 1, rPhl p 2, nPhl p 4, rPhl p 5, rPhl p 6, rPhl p 7, rPhl p 11, and rPhl p 12: epidemiological and diagnostic data. Clin Exp Allergy 2003 33 : 43 51.