A novel fluorescent α-conotoxin for the study of α7 nicotinic acetylcholine receptors

Journal of Neurochemistry

Volumn 111, Issue 1, 2009, Pages 80-89

  Hone, Arik J ^a   Whiteaker, Paul ^b   Christensen, Sean ^a   Xiao, Yingxian ^c   Meyer, Erin L ^a   McIntosh, J Michael ^a  

^a UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

^b BARROW NEUROLOGICAL INSTITUTE   (United States)

^c GEORGETOWN UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

conotoxin; 7 nicotinic acetylcholine receptor; Fluorescent; Human embryonic kidney 293 cells

Indexed keywords

ALPHA 7 NICOTINIC CHOLINERGIC RECEPTOR; BUNGAROTOXIN RECEPTOR; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ARTICLE; CHEMICAL STRUCTURE; ELECTROPHYSIOLOGY; FLUORESCENCE IMAGING; HIPPOCAMPUS; IC 50; MALE; MOUSE; NONHUMAN; OOCYTE; PRIORITY JOURNAL; XENOPUS LAEVIS;

ACETYLCHOLINE; ANIMALS; BINDING SITES; BINDING, COMPETITIVE; BIOPHYSICS; BUNGAROTOXINS; CARBACHOL; CELL LINE, TRANSFORMED; CHOLINERGIC AGONISTS; CHOLINERGIC ANTAGONISTS; COBRA NEUROTOXIN PROTEINS; CONOTOXINS; DOSE-RESPONSE RELATIONSHIP, DRUG; ELECTRIC STIMULATION; HUMANS; INHIBITORY CONCENTRATION 50; IODINE ISOTOPES; MEMBRANE POTENTIALS; MICROINJECTIONS; OOCYTES; PATCH-CLAMP TECHNIQUES; RADIOLIGAND ASSAY; RATS; RECEPTORS, NICOTINIC; TRANSFECTION; XENOPUS;

XENOPUS LAEVIS;

EID: 70349205445     PISSN: 00223042     EISSN: 14714159     Source Type: Journal    
DOI: 10.1111/j.1471-4159.2009.06299.x     Document Type: Article

References (51)

1. Arredondo J., Nguyen V. T., Chernyavsky A. I., Bercovich D., Orr-Urtreger A., Kummer W., Lips K., Vetter D. E. Grando S. A. (2002) Central role of alpha7 nicotinic receptor in differentiation of the stratified squamous epithelium. J. Cell Biol. 159, 325 336.
2. Basus V. J., Song G. Hawrot E. (1993) NMR solution structure of an alpha-bungarotoxin/nicotinic receptor peptide complex. Biochemistry 32, 12290 12298.
3. Biallas S., Wilker S., Lips K. S., Kummer W., Grando S. A., Padberg W. Grau V. (2007) Immunohistochemical detection of nicotinic acetylcholine receptor subunits alpha9 and alpha10 in rat lung isografts and allografts. Life Sci. 80, 2286 2289.
4. Cartier G. E., Yoshikami D., Gray W. R., Luo S., Olivera B. M. McIntosh J. M. (1996) A new alpha-conotoxin which targets alpha3beta2 nicotinic acetylcholine receptors. J. Biol. Chem. 271, 7522 7528.
5. Cheng Y. Prusoff W. H. (1973) Relationship between the inhibition constant (K1) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reaction. Biochem. Pharmacol. 22, 3099 3108.
6. Chernyavsky A. I., Arredondo J., Vetter D. E. Grando S. A. (2007) Central role of alpha9 acetylcholine receptor in coordinating keratinocyte adhesion and motility at the initiation of epithelialization. Exp. Cell Res. 313, 3542 3555.
7. Damaj M. I., Fei-Yin M., Dukat M., Glassco W., Glennon R. A. Martin B. R. (1998) Antinociceptive responses to nicotinic acetylcholine receptor ligands after systemic and intrathecal administration in mice. J. Pharmacol. Exp. Ther. 284, 1058 1065.
8. Damaj M. I., Fonck C., Marks M. J., Deshpande P., Labarca C., Lester H. A., Collins A. C. Martin B. R. (2007) Genetic approaches identify differential roles for alpha4beta2* nicotinic receptors in acute models of antinociception in mice. J. Pharmacol. Exp. Ther. 321, 1161 1169.
9. Dani J. A. Bertrand D. (2007) Nicotinic acetylcholine receptors and nicotinic cholinergic mechanisms of the central nervous system. Annu. Rev. Pharmacol. Toxicol. 47, 699 729.
10. Dvorakova M., Lips K. S., Bruggmann D., Slavikova J., Kuncova J. Kummer W. (2005) Developmental changes in the expression of nicotinic acetylcholine receptor alpha-subunits in the rat heart. Cell Tissue Res. 319, 201 209.
11. Elgoyhen A. B., Johnson D. S., Boulter J., Vetter D. E. Heinemann S. (1994) Alpha 9: an acetylcholine receptor with novel pharmacological properties expressed in rat cochlear hair cells. Cell 79, 705 715.
12. Ellison M., Haberlandt C., Gomez-Casati M. E., Watkins M., Elgoyhen A. B., McIntosh J. M. Olivera B. M. (2006) Alpha-RgIA: a novel conotoxin that specifically and potently blocks the alpha9alpha10 nAChR. Biochemistry 45, 1511 1517.
13. Genzen J. R. McGehee D. S. (2003) Short- and long-term enhancement of excitatory transmission in the spinal cord dorsal horn by nicotinic acetylcholine receptors. Proc. Natl Acad. Sci. USA 100, 6807 6812.
14. Genzen J. R., Van Cleve W. McGehee D. S. (2001) Dorsal root ganglion neurons express multiple nicotinic acetylcholine receptor subtypes. J. Neurophysiol. 86, 1773 1782.
15. Gerzanich V., Anand R. Lindstrom J. (1994) Homomers of alpha 8 and alpha 7 subunits of nicotinic receptors exhibit similar channel but contrasting binding site properties. Mol. Pharmacol. 45, 212 220.
16. Gomez-Casati M. E., Wedemeyer C., Taranda J., Lipovsek M., Dalamon V., Elgoyhen A. B. Katz E. (2009) Electrical properties and functional expression of ionic channels in cochlear inner hair cells of mice lacking the alpha10 nicotinic cholinergic receptor subunit. J. Assoc. Res. Otolaryngol. 10, 221 232.
17. Gotti C., Moretti M., Maggi R., Longhi R., Hanke W., Klinke N. Clementi F. (1997) Alpha7 and alpha8 nicotinic receptor subtypes immunopurified from chick retina have different immunological, pharmacological and functional properties. Eur. J. Neurosci. 9, 1201 1211.
18. Grau V., Wilker S., Hartmann P., Lips K. S., Grando S. A., Padberg W., Fehrenbach H. Kummer W. (2007) Administration of keratinocyte growth factor (KGF) modulates the pulmonary expression of nicotinic acetylcholine receptor subunits alpha7, alpha9 and alpha10. Life Sci. 80, 2290 2293.
19. Haberberger R. V., Bernardini N., Kress M., Hartmann P., Lips K. S. Kummer W. (2004) Nicotinic acetylcholine receptor subtypes in nociceptive dorsal root ganglion neurons of the adult rat. Auton. Neurosci. 113, 32 42. (Pubitemid 39024511)
20. Herber D. L., Severance E. G., Cuevas J., Morgan D. Gordon M. N. (2004) Biochemical and histochemical evidence of nonspecific binding of alpha7nAChR antibodies to mouse brain tissue. J. Histochem. Cytochem. 52, 1367 1376.
21. de Jonge W. J. Ulloa L. (2007) The alpha7 nicotinic acetylcholine receptor as a pharmacological target for inflammation. Br. J. Pharmacol. 151, 915 929.
22. Kawashima K. Fujii T. (2004) Expression of non-neuronal acetylcholine in lymphocytes and its contribution to the regulation of immune function. Front. Biosci. 9, 2063 2085.
23. Klink R., de Kerchove d'Exaerde A., Zoli M. Changeux J. P. (2001) Molecular and physiological diversity of nicotinic acetylcholine receptors in the midbrain dopaminergic nuclei. J. Neurosci. 21, 1452 1463.
24. Kumar P. Meizel S. (2005) Nicotinic acetylcholine receptor subunits and associated proteins in human sperm. J. Biol. Chem. 280, 25928 25935.
25. Lips K. S., Pfeil U. Kummer W. (2002) Coexpression of alpha 9 and alpha 10 nicotinic acetylcholine receptors in rat dorsal root ganglion neurons. Neuroscience 115, 1 5.
26. Lips K. S., Konig P., Schatzle K., Pfeil U., Krasteva G., Spies M., Haberberger R. V., Grando S. A. Kummer W. (2006) Coexpression and spatial association of nicotinic acetylcholine receptor subunits alpha7 and alpha10 in rat sympathetic neurons. J. Mol. Neurosci. 30, 15 16.
27. McIntosh J. M., Azam L., Staheli S., Dowell C., Lindstrom J. M., Kuryatov A., Garrett J. E., Marks M. J. Whiteaker P. (2004) Analogs of alpha-conotoxin MII are selective for alpha6-containing nicotinic acetylcholine receptors. Mol. Pharmacol. 65, 944 952.
28. McIntosh J. M., Plazas P. V., Watkins M., Gomez-Casati M. E., Olivera B. M. Elgoyhen A. B. (2005) A novel alpha-conotoxin, PeIA, cloned from Conus pergrandis, discriminates between rat alpha9alpha10 and alpha7 nicotinic cholinergic receptors. J. Biol. Chem. 280, 30107 30112.
29. Mogg A. J., Whiteaker P., McIntosh J. M., Marks M., Collins A. C. Wonnacott S. (2002) Methyllycaconitine is a potent antagonist of alpha-conotoxin-MII-sensitive presynaptic nicotinic acetylcholine receptors in rat striatum. J. Pharmacol. Exp. Ther. 302, 197 204.
30. Moser N., Mechawar N., Jones I. et al. (2007) Evaluating the suitability of nicotinic acetylcholine receptor antibodies for standard immunodetection procedures. J. Neurochem. 102, 479 492.
31. Olivera B. M., Quik M., Vincler M. McIntosh J. M. (2008) Subtype-selective conopeptides targeted to nicotinic receptors: concerted discovery and biomedical applications. Channels (Austin) 2, 143 152.
32. Palma E., Bertrand S., Binzoni T. Bertrand D. (1996) Neuronal nicotinic alpha 7 receptor expressed in Xenopus oocytes presents five putative binding sites for methyllycaconitine. J. Physiol. 491, 151 161.
33. Papadopolou S., Hartmann P., Lips K. S., Kummer W. Haberberger R. V. (2004) Nicotinic receptor mediated stimulation of NO-generation in neurons of rat thoracic dorsal root ganglia. Neurosci. Lett. 361, 32 35. (Pubitemid 38609209)
34. Peng H., Ferris R. L., Matthews T., Hiel H., Lopez-Albaitero A. Lustig L. R. (2004) Characterization of the human nicotinic acetylcholine receptor subunit alpha (alpha) 9 (CHRNA9) and alpha (alpha) 10 (CHRNA10) in lymphocytes. Life Sci. 76, 263 280.
35. Rau K. K., Johnson R. D. Cooper B. Y. (2005) Nicotinic AChR in subclassified capsaicin-sensitive and -insensitive nociceptors of the rat DRG. J. Neurophysiol. 93, 1358 1371.
36. Satkunanathan N., Livett B., Gayler K., Sandall D., Down J. Khalil Z. (2005) Alpha-conotoxin Vc1.1 alleviates neuropathic pain and accelerates functional recovery of injured neurones. Brain Res. 1059, 149 158.
37. Scheffer D., Sage C., Plazas P. V. et al. (2007) The alpha1 subunit of nicotinic acetylcholine receptors in the inner ear: transcriptional regulation by ATOH1 and co-expression with the gamma subunit in hair cells. J. Neurochem. 103, 2651 2664.
38. Sgard F., Charpantier E., Bertrand S., Walker N., Caput D., Graham D., Bertrand D. Besnard F. (2002) A novel human nicotinic receptor subunit, alpha10, that confers functionality to the alpha9-subunit. Mol. Pharmacol. 61, 150 159.
39. Taranda J., Maison S. F., Ballestero J. A. et al. (2009) A point mutation in the hair cell nicotinic cholinergic receptor prolongs cochlear inhibition and enhances noise protection. PLoS Biol. 7, e18.
40. Verbitsky M., Rothlin C. V., Katz E. Elgoyhen A. B. (2000) Mixed nicotinic-muscarinic properties of the alpha9 nicotinic cholinergic receptor. Neuropharmacology 39, 2515 2524.
41. Vetter D. E., Katz E., Maison S. F., Taranda J., Turcan S., Ballestero J., Liberman M. C., Elgoyhen A. B. Boulter J. (2007) The alpha10 nicotinic acetylcholine receptor subunit is required for normal synaptic function and integrity of the olivocochlear system. Proc. Natl Acad. Sci. USA 104, 20594 20599.
42. Vincler M. McIntosh J. M. (2007) Targeting the alpha9alpha10 nicotinic acetylcholine receptor to treat severe pain. Expert Opin. Ther. Targets 11, 891 897.
43. Vincler M., Wittenauer S., Parker R., Ellison M., Olivera B. M. McIntosh J. M. (2006) Molecular mechanism for analgesia involving specific antagonism of alpha9alpha10 nicotinic acetylcholine receptors. Proc. Natl Acad. Sci. USA 103, 17880 17884.
44. Wang H., Yu M., Ochani M. et al. (2003) Nicotinic acetylcholine receptor alpha7 subunit is an essential regulator of inflammation. Nature 421, 384 388.
45. Whiteaker P., Christensen S., Yoshikami D., Dowell C., Watkins M., Gulyas J., Rivier J., Olivera B. M. McIntosh J. M. (2007) Discovery, synthesis, and structure activity of a highly selective alpha7 nicotinic acetylcholine receptor antagonist. Biochemistry 46, 6628 6638.
46. Whiteaker P., Marks M. J., Christensen S., Dowell C., Collins A. C. McIntosh J. M. (2008) Synthesis and characterization of 125I-alpha-conotoxin ArIB[V11L;V16A], a selective alpha7 nicotinic acetylcholine receptor antagonist. J. Pharmacol. Exp. Ther. 325, 910 919.
47. Wilkins M. E., Li X. Smart T. G. (2008) Tracking cell surface GABAB receptors using an alpha-bungarotoxin tag. J. Biol. Chem. 283, 34745 34752.
48. Xiao Y. Kellar K. J. (2004) The comparative pharmacology and up-regulation of rat neuronal nicotinic receptor subtype binding sites stably expressed in transfected mammalian cells. J. Pharmacol. Exp. Ther. 310, 98 107.
49. Xiao Y., Meyer E. L., Thompson J. M., Surin A., Wroblewski J. Kellar K. J. (1998) Rat alpha3/beta4 subtype of neuronal nicotinic acetylcholine receptor stably expressed in a transfected cell line: pharmacology of ligand binding and function. Mol. Pharmacol. 54, 322 333.
50. Xiao Y., Abdrakhmanova G. R., Baydyuk M., Hernandez S. Kellar K. J. (2009) Rat neuronal nicotinic acetylcholine receptors containing alpha7 subunit: pharmacological properties of ligand binding and function. Acta Pharmacol. Sin. 30, 842 850.
51. Zorrilla de San Martin J., Ballestero J., Katz E., Elgoyhen A. B. Fuchs P. A. (2007) Ryanodine is a positive modulator of acetylcholine receptor gating in cochlear hair cells. J Assoc Res Otolaryngol 8, 474 483.