Highly efficient subcloning of rodent malaria parasites by injection of single merosomes or detached cells

Nature Protocols

Volumn 4, Issue 10, 2009, Pages 1433-1439

  Stanway, Rebecca R ^a   Graewe, Stefanie ^a   Rennenberg, Annika ^a   Helm, Susanne ^a   Heussler, Volker T ^a  

^a BERNHARD NOCHT INSTITUTE FOR TROPICAL MEDICINE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; HEPATOMA CELL; IN VITRO STUDY; LARVAL STAGE; MEROZOITE; MOLECULAR CLONING; MOUSE; NONHUMAN; PLASMODIUM BERGHEI; PRIORITY JOURNAL; RODENT MALARIA; SPOROZOITE;

ANIMALS; CELL LINE, TUMOR; HUMANS; INJECTIONS; MALARIA; MICE; PARASITOLOGY; PLASMODIUM BERGHEI;

MUS; PLASMODIUM BERGHEI; PLASMODIUM PARASITES; RODENTIA;

EID: 70349124173     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2009.172     Document Type: Article

References (28)

1. de Koning-Ward, T.F. et al. The selectable marker human dihydrofolate reductase enables sequential genetic manipulation of the Plasmodium berghei genome. Mol. Biochem. Parasitol. 106 199-212 (2000).
2. Janse, C.J. et al. High efficiency transfection of Plasmodium berghei facilitates novel selection procedures. Mol. Biochem. Parasitol. 145, 60-70 (2006).
3. Janse, C.J., Ramesar, J. & Waters, A.P. High-efficiency transfection and drug selection of genetically transformed blood stages of the rodent malaria parasite Plasmodium berghei. Nat. Protoc. 1, 346-356 (2006).
4. Menard, R. & Janse, C. Gene targeting in malaria parasites. Methods 13, 148-157 (1997).
5. Thathy, V. & Menard, R. Gene targeting in Plasmodium berghei. Methods Mol. Med. 72, 317-331 (2002).
6. Jongco, A.M., Ting, L.M., Thathy, V., Mota, M.M. & Kim, K. Improved transfection and new selectable markers for the rodent malaria parasite Plasmodium yoelii. Mol. Biochem. Parasitol. 146, 242-250 (2006).
7. Mota, M.M., Thathy, V., Nussenzweig, R.S. & Nussenzweig, V. Gene targeting in the rodent malaria parasite Plasmodium yoelii. Mol. Biochem. Parasitol. 113, 271-278 (2001).
8. Sultan, A.A., Thathy, V., Nussenzweig, V. & Menard, R. Green fluorescent protein as a marker in Plasmodium berghei transformation. Infect. Immun. 67, 2602-2606 (1999).
9. Natarajan, R. et al. Fluorescent Plasmodium berghei sporozoites and pre-erythrocytic stages: A new tool to study mosquito and mammalian host interactions with malaria parasites. Cell. Microbiol. 3, 371-379 (2001).
10. Franke-Fayard, B. et al. A Plasmodium berghei reference line that constitutively expresses GFP at a high level throughout the complete life cycle. Mol. Biochem. Parasitol. 137, 23-33 (2004).
11. Frevert, U. et al. Intravital observation of Plasmodium berghei sporozoite infection of the liver. PLoS Biol. 3, e192 (2005).
12. Amino, R. et al. Quantitative imaging of Plasmodium transmission from mosquito to mammal. Nat. Med. 12, 220-224 (2006).
13. Amino, R. et al. Imaging malaria sporozoites in the dermis of the mammalian host. Nat. Protoc. 2, 1705-1712 (2007).
14. Thiberge, S. et al. In vivo imaging of malaria parasites in the murine liver. Nat. Protoc. 2, 1811-1818 (2007).
15. Ono, T., Tadakuma, T. & Rodriguez, A. Plasmodium yoelii yoelii 17XNL constitutively expressing GFP throughout the life cycle. Exp. Parasitol. 115, 310-313 (2007).
16. Sturm, A. et al. Alteration of the parasite plasma membrane and the parasitophorous vacuole membrane during exo-erythrocytic development of malaria parasites. Protist 160, 51-63 (2009).
17. Stanway, R.R., Witt, T., Zobiak, B., Aepfelbacher, M. & Heussler, V.T. GFP-targeting allows visualization of the apicoplast throughout the life cycle of live malaria parasites. Biol. Cell 101, 415-430 (2009).
18. Graewe, S., Retzlaff, S., Struck, N., Janse, C.J. & Heussler, V.T. Going live: A comparative analysis of the suitability of the RFP derivatives RedStar, mCherry and tdTomato for intravital and in vitro live imaging of Plasmodium parasites. Biotechnol. J. 4, 895-902 (2009).
19. Sturm, A. et al. Manipulation of host hepatocytes by the malaria parasite for delivery into liver sinusoids. Science 313, 1287-1290 (2006).
20. Mueller, A.K. et al. Plasmodium liver stage developmental arrest by depletion of a protein at the parasite-host interface. Proc. Natl. Acad. Sci. USA 102, 3022-3027 (2005).
21. Mueller, A.K., Labaied, M., Kappe, S.H. & Matuschewski, K. Genetically modified Plasmodium parasites as a protective experimental malaria vaccine. Nature 433, 164-167 (2005).
22. van Dijk, M.R. et al. Genetically attenuated, P36p-deficient malarial sporozoites induce protective immunity and apoptosis of infected liver cells. Proc. Natl. Acad. Sci. USA 102, 12194-12199 (2005).
23. Janse, C. & Waters, A.P. In Malaria Methods and Protocols (ed. Doolan, D.L.) 311-312 (Humana Press Inc., Totowa, New Jersey, 2002).
24. Meissner, M., Brecht, S., Bujard, H. & Soldati, D. Modulation of myosin A expression by a newly established tetracycline repressor-based inducible system in Toxoplasma gondii. Nucleic Acids Res. 29 E115 (2001).
25. Armstrong, C.M. & Goldberg, D.E. An FKBP destabilization domain modulates protein levels in Plasmodium falciparum. Nat. Methods 4, 1007-1009 (2007).
26. Herm-Gotz, A. et al. Rapid control of protein level in the apicomplexan Toxoplasma gondii. Nat. Methods 4, 1003-1005 (2007).
27. Waters, A.P., Thomas, A.W., van Dijk, M.R. & Janse, C.J. Transfection of malaria parasites. Methods 13, 134-147 (1997).
28. Silvie, O., Goetz, K. & Matuschewski, K. A sporozoite asparagine-rich protein controls initiation of Plasmodium liver stage development. PLoS Pathog. 4, e1000086 (2008).