Attachment of stem cells to scaffold particles for intra-cerebral transplantation

Nature Protocols

Volumn 4, Issue 10, 2009, Pages 1440-1453

  Bible, Ellen ^a   Chau, David Y S ^b   Alexander, Morgan R ^b   Price, Jack ^a   Shakesheff, Kevin M ^b   Modo, Michel ^a  

^a KING'S COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

FIBRONECTIN; POLYGLACTIN; SCAFFOLD PROTEIN;

ANIMAL CELL; ARTICLE; BRAIN TRANSPLANTATION; CELL ADHESION; CELL AGGREGATION; CONTROLLED STUDY; HISTOLOGY; NONHUMAN; PRIORITY JOURNAL; STEM CELL TRANSPLANTATION; TISSUE REGENERATION; TRAUMATIC BRAIN INJURY;

ANIMALS; CEREBRUM; FIBRONECTINS; RATS; RATS, SPRAGUE-DAWLEY; STEM CELL TRANSPLANTATION; SURFACE PROPERTIES; TISSUE SCAFFOLDS; TISSUE THERAPY;

EID: 70349084163     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2009.156     Document Type: Article

References (15)

1. Bjorklund, A. & Lindvall, O. Cell replacement therapies for central nervous system disorders. Nat. Neurosci. 3, 537-544 (2000).
2. Aebischer, P., Tresco, P.A., Winn, S.R., Greene, L.A. & Jaeger, C.B. Long-term cross-species brain transplantation of a polymer-encapsulated dopamine-secreting cell line. Exp. Neurol. 111, 269-275 (1991).
3. Aebischer, P., Wahlberg, L., Tresco, P.A. & Winn, S.R. Macroencapsulation of dopamine-secreting cells by coexclusion with an organic polymer solution. Biomaterials 12, 50-56 (1991).
4. Lavik, E.B., Klassen, H., Warfvinge, K., Langer, R. & Young, M.J. Fabrication of degradable polymer scaffolds to direct the integration and differentiation of retinal progenitors. Biomaterials 26, 3187-3196 (2005).
5. Park, K.I., Teng, Y.D. & Snyder, E.Y. The injured brain interacts reciprocally with neural stem cells supported by scaffolds to reconstitute lost tissue. Nat. Biotechnol. 20, 1111-1117 (2002).
6. Tomita, M. et al. Biodegradable polymer composite grafts promote the survival and differentiation of retinal progenitor cells. Stem Cells 23, 1579-1588 (2005).
7. Bible, E. et al. The support of neural stem cells transplanted into stroke-induced brain cavities by PLGA particles. Biomaterials 30, 2985-2994 (2009).
8. Hejcl A. et al. Biocompatible hydrogels in spinal cord injury repair. Physiol. Res. 57 (Suppl 3): S121-132 (2008).
9. Teng, Y.D. et al. Functional recovery following traumatic spinal cord injury mediated by a unique polymer scaffold seeded with neural stem cells. Proc. Natl. Acad. Sci. USA 99, 3024-3029 (2002).
10. Sinden, J.D. et al. Recovery of spatial learning by grafts of a conditionally immortalized hippocampal neuroepithelial cell line into the ischaemia-lesioned hippocampus. Neuroscience 81, 599-608 (1997).
11. Modo, M., Stroemer, R.P., Tang, E., Patel, S. & Hodges, H. Effects of implantation site of dead stem cells in rats with stroke damage. Neuroreport 14, 39-42 (2003).
12. Modo, M. et al. Neurological sequelae and long-term behavioural assessment of rats with transient middle cerebral artery occlusion. J. Neurosci. Methods 104, 99-109 (2000).
13. Modo, M., Stroemer, R.P., Tang, E., Patel, S. & Hodges, H. Effects of implantation site of stem cell grafts on behavioral recovery from stroke damage. Stroke 33, 2270-2278 (2002).
14. Paxinos, G. & Watson, C. The Rat Brain in Stereotaxic Coordinates 6th edn. (Elsevier, Amsterdam, NL, 2007).
15. Zetzer, M. et al. Investigation of cell-surface interactions using chemical gradients formed from plasma polymers. Biomaterials 29 172-184 (2008).