Solvent free synthesis of 1,5-disubstituted tetrazoles derived from Baylis Hillman acetates as potential TNF-α inhibitors

Bioorganic and Medicinal Chemistry Letters

Volumn 19, Issue 19, 2009, Pages 5569-5572

  Srihari, P ^a   Dutta, Palash ^a   Rao, R Srinivasa ^a   Yadav, J S ^a   Chandrasekhar, S ^a   Thombare, P ^b   Mohapatra, J ^b   Chatterjee, A ^b   Jain, Mukul R ^b  

^a INDIAN INSTITUTE OF CHEMICAL TECHNOLOGY   (India)

^b ZYDUS RESEARCH CENTRE   (India)

Author keywords

Arthritis; Baylis Hillman; Click chemistry; Michael reaction; Multicomponent

Indexed keywords

ACETIC ACID; AZIDE; TETRAZOLE DERIVATIVE; TUMOR NECROSIS FACTOR ALPHA INHIBITOR;

ARTICLE; BAYLIS HILLMAN REACTION; HUMAN; HUMAN CELL; ONE POT SYNTHESIS; RHEUMATOID ARTHRITIS;

ACETATES; ANTI-INFLAMMATORY AGENTS; HUMANS; SOLVENTS; TETRAZOLES; TUMOR NECROSIS FACTOR-ALPHA;

EID: 70149123806     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bmcl.2009.08.047     Document Type: Article

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24. 3 (1 mmol) were stirred at room temperature for half an hour and to this mixture was added aryl nitrile (1 mmol) and the mixture was heated to 85 °C till completion of the reaction (as given in Table 1). The compound was diluted with ethyl acetate and water was added. The aqueous layer was extracted with ethyl acetate. The solvent was evaporated under reduced pressure and the crude product was purified by column chromatography.
25. 2
26. The diastereomeric mixture of a representative sample (3j) was separated and their respective geometries were characterized by NOE studies.
27. Procedure for assay: inhibition of TNF-α in human whole blood assay: fresh blood was collected aseptically in the presence of heparin by venipuncture from healthy adult volunteers. Two microliters of either a test compound solution (10, 100 μM) or dimethyl sulfoxide was mixed with 246-μl aliquot of blood and incubated at 37 °C for 1 h. Following this, 2 μl of 125 ng/ml lipopolysaccharide (dissolved in phosphate-buffered saline; final concentration of 1 ng/ml) were added in each microtube. The blood mixture along with LPS was further incubated at 37 °C for 5 h. The reactions were terminated by placing the samples over ice for 10 min. At study completion, the plasma was separated by centrifugation at 3000 rpm for 10 min at 4 °C and stored at -70 °C until further analysis. Concentrations of tumor necrosis factor-alpha in the plasma were determined by enzyme-linked immunosorbent assays (BD Biosciences, USA).