Assessment of genotoxic damage in nurses occupationally exposed to anaesthetic gases or antineoplastic drugs by the comet assay

Journal of Occupational Health

Volumn 51, Issue 3, 2009, Pages 283-286

  Izdes, Seval ^a   Sardas, Semra ^b   Kadioglu, Ela ^c   Kaymak, Cetin ^d   Ozcagli, Eren ^c  

^a ATATURK TRAINING AND RESEARCH HOSPITAL   (Turkey)

^b MARMARA UNIVERSITY   (Turkey)

^c GAZI UNIVERSITY   (Turkey)

^d KIRIKKALE UNIVERSITY   (Turkey)

Author keywords

Anaesthetics; Antineoplastics; Comet assay; DNA damage; Occupational exposure

Indexed keywords

ANTINEOPLASTIC AGENT; INHALATION ANESTHETIC AGENT;

ADULT; ARTICLE; COMET ASSAY; DNA DAMAGE; DRUG EFFECT; FEMALE; HUMAN; MALE; MIDDLE AGED; NURSE; OCCUPATIONAL EXPOSURE;

ADULT; ANESTHETICS, INHALATION; ANTINEOPLASTIC AGENTS; COMET ASSAY; DNA DAMAGE; FEMALE; HUMANS; MALE; MIDDLE AGED; NURSES; OCCUPATIONAL EXPOSURE;

EID: 69949101503     PISSN: 13419145     EISSN: 13489585     Source Type: Journal    
DOI: 10.1539/joh.M8012     Document Type: Article

References (20)

1. Wiesner G, Hoerauf K, Schroegendorfer K, Sobczynski P, Harth M, Reudiger HW. High-level, but not low-level, occupational exposure to inhaled anaesthetics is associated with genotoxicity in the micronucleus assay. Anesth Analg 2001; 92: 118-22.
2. Kopjar N, Garaj-Vrhovac V. Application of the alkaline comet assay in human biomonitoring for genotoxicity: A study on Croatian medical personnel handling antineoplastic drugs. Mutagenesis 2001; 16: 71-8.
3. Sharbel WM, Erdtmann B. Follow-up study of the genetic damage in lymphocytes of pharmacists and nurses handling antineoplastic drugs evaluated by cytokinesis-block micronuclei analysis and single cell gel electrophoresis assay. Mutat Res 2000; 471: 21-7.
4. Calabresi P, Chabner BA. Chemotherapy of neoplastic disease. In:. Hardman JG, Limbird LE, editors. Goodman and Gilman's: The pharmacological basis of therapeutics. New York: The McGraw-Hill Companies; 2001. p. 1381-461.
5. Buring JE, Hennekens CH, Mayrent SL, Rosner B, Greenberg ER, Colton T. Health experiences of operating room personnel. Anaesthesiology 1985; 62: 325-30.
6. Sardas S, Izdes S, Ozcagli E, Kanbak O, Kadioglu E. The role of antioxidant supplementation in occupational exposure to waste anaestetic gases. Int Arch Occup Environ Health 2006; 80: 154-9.
7. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 1988; 175: 184-91.
8. Collins AR. The comet assay. Principles, applications, and limitations. Meth Mol Biol 2002; 203: 163-77.
9. Laffon B, Teixeira JP, Silva S, et al. Genotoxic effects in a population of nurses handling antineoplastic drugs, and relationship with genetic polymorphisms in DNA repair enzymes. Am J Ind Med 2005; 48: 128-36.
10. Maluf SW, Erdtmann B. Follow-up study of the genetic damage in lymphocytes of pharmacists and nurses handling antineoplastic drugs evaluated by cytokinesisblock micronuclei analysis and single cell gel electrophoresis assay. Mutat Res 2000; 471: 21-7.
11. Sasaki M, Dakeishi M, Hoshi S, Ishii N, Murata K. Assessment of DNA damage in Japanese nurses handling antineoplastic drugs by the comet assay. J Occup Health 2008; 50: 7-12.
12. Burgaz S, Özdamar YN, Karakaya AE. A signal assay for the detection of genotoxic compounds: Application on the urines of cancer patients on chemotherapy and of nurses handling cytotoxic drugs. Human Toxicol 1988; 7: 557-60.
13. Sardas S, Gök S, Karakaya AE. Sister chromatid exchange in lymphocytes of nurses handling antineoplastic drugs. Toxicol Lett 1991; 55: 311-5.
14. Undeger U, Basaran N, Kars A, Guc D. Assesment of DNA damage in nurses handling antineoplastic drugs by alkaline comet assay. Mutat Res 1999; 439: 277-85.
15. Rozgaj R, Kasuba V, Jazbec A. Preliminary study of cytogenetic damage in personnel exposed to anaesthetic gases. Mutagenesis 2001; 16: 139-43.
16. Chandrasekhar M, Rekhadevi PV, Sailaja N, et al. Evaluation of genetic damage in operating room personnel exposed to anaesthetic gases. Mutagenesis 2006; 21: 249-54.
17. Sardas S, Aygün N, Gamli M, Ünal Y, Ünal N, Berk N. Use of alkaline Comet assay (single cell gel electrophoresis technique) to detect DNA damages in lymphocytes of operating room personnel occupationally exposed to anaesthetic gases. Mutat Res 1998; 418: 93-100.
18. Hoerauf K, Funk W, Harth M, Hobbhahn J. Occupational exposure to sevoflurane, halothane and nitrous oxide during paediatric anaesthesia. Waste gas exposure during pediatric anaesthesia. Anaesthesia 1997; 52: 215-9.
19. Moller P, Knudsen LE, Loft S, Wallin H. The comet assay as a rapid test in biomonitoring occupational exposure to DNA-damaging agents and effect of confounding factors. Cancer Epidemiol Biomarkers Prev 2000; 9: 1005-15.
20. Speit G, Witton-Davies T, Heepchantree W, Trenz K, Hoffmann H. Investigations on the effect of cigarette smoking in the comet assay. Mutat Res 2003; 542: 33-42.