Prospective comparison of eubacterial PCR and measurement of procalcitonin levels with blood culture for diagnosing septicemia in intensive care unit patients

Journal of Clinical Microbiology

Volumn 47, Issue 9, 2009, Pages 2964-2969

  Rowther, Farjana B ^a   Rodrigues, Camilla S ^a   Deshmukh, Minal S ^a   Kapadia, Farhad N ^a   Hegde, Ashit ^a   Mehta, Ajita P ^a   Joshi, Vinay R ^a  

^a P D HINDUJA NATIONAL HOSPITAL   (India)

Author keywords

[No Author keywords available]

Indexed keywords

PROCALCITONIN;

ADULT; AGED; ARTICLE; BLOOD CULTURE; CASE CONTROL STUDY; CONTROLLED STUDY; CRITICALLY ILL PATIENT; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; EARLY DIAGNOSIS; EUBACTERIUM; FEMALE; GOLD STANDARD; HUMAN; INTENSIVE CARE UNIT; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MALE; POLYMERASE CHAIN REACTION; PREDICTIVE VALIDITY; PRIORITY JOURNAL; PROSPECTIVE STUDY; SENSITIVITY AND SPECIFICITY; SEPTICEMIA;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; BACTERIA; CALCITONIN; DNA, BACTERIAL; FEMALE; HUMANS; INTENSIVE CARE UNITS; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE OF TESTS; PROTEIN PRECURSORS; SENSITIVITY AND SPECIFICITY; SEPSIS; YOUNG ADULT;

BACTERIA (MICROORGANISMS);

EID: 69949100886     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.00418-07     Document Type: Article

References (30)

1. Assicot, M., D. Gendrel, H. Carsin, J. Raymond, G. Guilbaud, and C. Bohuon. 1993. High serum procalcitonin concentrations in patients with sepsis and infection. Lancet 341:515-518. (Pubitemid 23066085)
2. Baue, A. E. 1993. The role of gut in the development of multiple organ dysfunction in cardiothoracic patients. Ann. Thorac. Surg. 55:822-829.
3. Bone, R. C. 1992. Epidemiology and natural history of SIRS (systemic in- flammatory response syndrome). JAMA 269:3452-3455.
4. Bone, R. C., R. A. Balk, F. B. Cerra, et al. 1992. American College of Chest Physician/Society of Critical Care Medicine Consensus Conference: definition for sepsis and organ failure and guidelines for the use of innovative therapies in sepsis. Crit. Care Med. 20:864-874.
5. Carrigan, S. D., G. Scott, and M. Tabrizian. 2004. Towards resolving the challenges of sepsis diagnosis. Clin. Chem. 50:1301-1314.
6. Castelli, G. P., C. Pognani, M. Cita, A. Stuani, L. Sgarbi, and R. Paladini. 2006. Procalcitonin C-reactive protein, white blood cells and SOFA score in ICU: diagnosis and monitoring sepsis. Minerva Anestesiol. 72:69-80.
7. Cockerill, F. R., III, J. W. Wilson, E. A. Vetter, et al. 2004. Optimal testing parameters for blood cultures. Clin. Iinfect. Dis. 38:1724-1730.
8. Cursons, R. T., E. Jeyerajah, and J. W. Sleigh. 1999. The use of polymerase chain reaction to detect septicemia in critically ill patients. Crit. Care Med. 27:937-940.
9. Hugenholtz, P., and T. Huber. 2003. Chimeric 16S rDNA sequences of diverse origin are accumulating in the public databases. Int. J. Syst. Evol. Microbiol. 53:289-293.
10. Kane, T. D., J. W. Alexander, and J. A. Johannigman. 1998. The detection of microbial DNA in the blood: a sensitive method for diagnosing bacteremia and/or bacterial translocation in surgical patients. Ann. Surg. 227:1-9.
11. Kane, T. D., S. R. Johnson, J. W. Alexander, et al. 1996. Detection of intestinal bacterial translocation using PCR. J. Surg. Res. 63:59-63.
12. Lee, A., S. Mirrett, L. B. Reller, and M. P. Weinstein. 2007. Detection of bloodstream infections in adults: how many blood cultures are needed? J. Clin. Microbiol. 45:3546-3548.
13. Mathur, P., A. Kapil, and B. Das. 2005. Nosocomial bacteremia in intensive care unit patients of a tertiary care center. Indian J. Med. Res. 122:305-308.
14. Matot, I., and C. L. Sprung. 2001. Definition of sepsis. Intensive Care Med. 27:S3-S9.
15. Meisner, M. 2002. Pathobiochemistry and clinical use of procalcitonin. Clin. Chim. Acta 323:17-29.
16. Meisner, M., K. Tschaikowsky, T. Palmaers, and J. Schmidt. 1999. Comparison of procalcitonin (PCT) and C-reactive protein (CRP) plasma concentrations at different SOFA scores during the course of sepsis and MODS. Crit. Care 3:45-50. (Pubitemid 29304058)
17. Michalopoulos, A., G. Stavridis, and S. Geroulanos. 1998. Severe sepsis in cardiac surgical patients. Eur. J. Surg. 164:217-222.
18. Millar, C. B., J. Xu, and J. E. Moore. 2002. Risk assessment models and communication management: implications for broad-range ribosomal DNA PCR as a diagnostic tool in medical bacteriology. J. Clin. Microbiol. 40:1575-1580.
19. Mitaka, C. 2005. Clinical laboratory differentiation of infectious versus noninfectious systemic inflammatory response syndrome. Clin. Chim. Acta 351:17-29.
20. Reno, W., III, D. O. McDaniel, W. W. Turner, Jr., and M. D. Williams. 2001. Polymerase chain reaction for the detection of bacteremia. Am. Surg. 67:508-512.
21. Rowther, F. B., C. S. Rodrigues, A. P. Mehta, et al. 2005. An improved method of elimination of DNA from PCR reagents. Mol. Diagn. 9:53-57.
22. Simon, L., F. Gauvin, D. K. Amre, P. Saint-Louis, and J. Lacroix. 2004. Serum procalcitonin and C-reactive protein levels as markers of bacterial infection: a systematic review and meta-analysis. Clin. Infect. Dis. 39:206-217.
23. Sponholz, C., Y. Sakr, K. Reinhart, and F. Brunkhorst. 2006. Diagnostic value and prognostic implications of serum procalcitonin after cardiac surgery: a systematic review of the literature. Crit. Care 10:1-11. (Pubitemid 47086948)
24. Suprin, E., C. Camus, A. Gacouin, et al. 2000. Procalcitonin: a valuable indicator of infection in a medical ICU? Intensive Care Med. 26:1232-1238.
25. Takai, S., T. Kuriyama, M. Yanagisawa, K. Nakagawa, and T. Karasawa. 2005. Incidence and bacteriology of bacteremia associated with various oral and maxillofacial surgical procedures. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 99:292-298.
26. Tang, B. M. P., G. D. Eslick, J. C. Craig, and A. S. McLean. 2007. Accuracy of procalcitonin for sepsis diagnosis in critically ill patients: systematic review and meta-analysis. Lancet Infect. Dis. 7:210-217.
27. von Landenberg, P., and Y. Shoenfeld. 2001. New approaches in the diagnosis of sepsis. Isr. Med. Assoc. J. 3:439-441.
28. Wellinghausen, N., B. Wirths, A. R. Franz, L. Karolyi, R. Marre, and U. Reischl. 2004. Algorithm for the identification of bacterial pathogens in positive blood cultures by real- time LightCycler polymerase chain reaction (PCR) with sequence specific probes. Diagn. Microbiol. Infect. Dis. 48:229-241.
29. Whicher, J., J. Bienvenu, and G. Monneret. 2001. Procalcitonin as an acute phase marker. Ann. Clin. Biochem. 38:483-493.
30. Wilson, M. L. 1994. Blood cultures. Introduction. Clin. Lab. Med. 14:1-7.