Effects of methodological variation on assessment of riboflavin status using the erythrocyte glutathione reductase activation coefficient assay

British Journal of Nutrition

Volumn 102, Issue 2, 2009, Pages 273-278

  Hill, Marilyn H E ^a   Bradley, Angela ^a   Mushtaq, Sohail ^a   Williams, Elizabeth A ^a   Powers, Hilary J ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

Erythrocyte glutathione reductase activation coefficient; Methodology; Nutrition surveys; Riboflavin status

Indexed keywords

FLAVINE ADENINE NUCLEOTIDE; RIBOFLAVIN; GLUTATHIONE REDUCTASE;

ADULT; ANALYTIC METHOD; ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; ERYTHROCYTE GLUTATHIONE REDUCTASE ACTIVATION COEFFICIENT ASSAY; FEMALE; HEALTH SURVEY; HUMAN; INCUBATION TIME; LABORATORY TEST; MAJOR CLINICAL STUDY; MALE; PREVALENCE; RIBOFLAVIN DEFICIENCY; UNITED KINGDOM; VITAMIN BLOOD LEVEL; BLOOD; COMPARATIVE STUDY; DIET; ENZYME ACTIVATION; ENZYME ASSAY; ENZYMOLOGY; ERYTHROCYTE; METABOLISM; METHODOLOGY; MIDDLE AGED; NONPARAMETRIC TEST; NUTRITION; NUTRITIONAL STATUS; REFERENCE VALUE; SENSITIVITY AND SPECIFICITY; STANDARD;

ADULT; CLINICAL ENZYME TESTS; DIET; ENZYME ACTIVATION; ERYTHROCYTES; FEMALE; GLUTATHIONE REDUCTASE; GREAT BRITAIN; HUMANS; MALE; MIDDLE AGED; NUTRITION SURVEYS; NUTRITIONAL STATUS; REFERENCE VALUES; RIBOFLAVIN; RIBOFLAVIN DEFICIENCY; SENSITIVITY AND SPECIFICITY; STATISTICS, NONPARAMETRIC; YOUNG ADULT;

EID: 69849101953     PISSN: 00071145     EISSN: 14752662     Source Type: Journal    
DOI: 10.1017/S0007114508162997     Document Type: Article

References (20)

1. Gregory J, Foster K, Tyler H, et al. (1990) The Dietary and Nutritional Survey of British Adults. London: The Stationery Office.
2. Rushton D, Hoare J, Henderson L, et al. (2003) The National Diet and Nutrition Survey: Adults Aged 19-24, vol.4. London: Office for National Statistics.
3. Bayoumi RA & Rosalki SB (1976) Evaluation of methods of coenzyme activation of erythrocyte enzymes for detection of deficiency of vitamins B1, B2, and B6. Clin Chem 22, 327-335.
4. Beutler E (1969) Effect of flavin compounds on glutathione reductase activity: in vivo and in vitro studies. J Clin Invest 48, 1957-1966.
5. Glatzle D, Korner WF, Christeller S, et al. (1970) Method for the detection of a biochemical riboflavin deficiency. Stimulation of NADPH2-dependent glutathione reductase from human erythrocytes by FAD in vitro. Investigations on the vitamin B2 status in healthy people and geriatric patients. Int Z Vitaminforsch 40, 166-183.
6. Nichoalds GE (1974) Assessment of status riboflavin nutriture by assay of erythrocyte glutathione reductase activity. Clin Chem 20, 624-628.
7. Thurnham DI & Rathakette P (1982) Incubation of NAD(P)H2: glutathione oxidoreductase (EC 1.6.4.2) with flavin adenine dinucleotide for maximal stimulation in the measurement of riboflavin status. Br J Nutr 48, 459-466.
8. Tillotson JA & Baker EM (1972) An enzymatic measurement of the riboflavin status in man. Am J Clin Nutr 25, 425-431.
9. Powers HJ, Bates CJ, Prentice AM, et al. (1983) The relative effectiveness of iron and iron with riboflavin in correcting a microcytic anaemia in men and children in rural Gambia. Hum Nutr Clin Nutr 37, 413-425.
10. Bingham SA, Gill C, Welch A, et al. (1997) Validation of dietary assessment methods in the UK arm of EPIC using weighed records, and 24-hour urinary nitrogen and potassium and serum vitamin C and carotenoids as biomarkers. Int J Epidemiol 26, Suppl. 1, S137-S151.
11. Holland B, Welch AA, Unwin ID, et al. (1991) McCance and Widdowson's The Composition of Foods, 5th ed. Cambridge: Ministry of Agriculture, Fisheries and Food/Royal Society of Chemistry.
12. Thurnham DI, Migasena P & Pavapootanon N (1970) The ultramicro red-cell glutathione reductase assay for riboflavin status: its use in field studies in Thailand. Mikrochim Acta 58, 988-993.
13. Garry PJ & Owen GM (1976) An automated flavin adenine dinucleotide-dependent glutathione reductase assay for assessing riboflavin nutriture. Am J Clin Nutr 29, 663-674.
14. Schorah CJ & Messenger D (1975) Flavin nucleotides, glutathione reductase and assessment of riboflavin status. Int J Vitam Nutr Res 45, 39-50.
15. Hartman HA, Edmondson DE & McCormick DB (1992) Riboflavin 50-pyrophosphate: a contaminant of commercial FAD, a coenzyme for FAD-dependent oxidases, and an inhibitor of FAD synthetase. Anal Biochem 202, 348-355.
16. Dewitte K, Fierens C, Stockl D, et al. (2002) Application of the Bland-Altman plot for interpretation of method-comparison studies: a critical investigation of its practice. Clin Chem 48, 799-801, author reply 801-1792
17. Bland JM & Altman DG (1986) Statistical methods for assessing agreement between two methods of clinical measurement. Lancet i, 307-310.
18. Dainty JR, Bullock NR, Hart DJ, et al. (2007) Quantification of the bioavailability of riboflavin from foods by use of stable-isotope labels and kinetic modeling. Am J Clin Nutr 85, 1557-1564.
19. Angeline T, Jeyaraj N & Tsongalis GJ (2007) MTHFR gene polymorphisms, B-vitamins and hyperhomocystinemia in young and middle-aged acute myocardial infarction patients. Exp Mol Pathol 82, 227-233.
20. Burtis CA, Ashwood ER & Tietz NW (editors) (1999) Tietz Textbook of Clinical Chemistry, 3rd ed. London: W.B. Saunders.