Low-intensity pulsed ultrasound stimulates cell proliferation, proteoglycan synthesis and expression of growth factor-related genes in human nucleus pulposus cell line

European Cells and Materials

Volumn 17, Issue , 2009, Pages 15-22

  Kobayashi, Yuka ^a   Sakai, Daisuke ^a   Iwashina, Toru ^a   Iwabuchi, Sadahiro ^b   Mochida, Joji ^a  

^a TOKAI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b Tejin Pharma Ltd   (Japan)

Author keywords

Growth factor; Intervertebral disc; LIPUS; Ultrasound

Indexed keywords

COLLAGEN; EPIDERMAL GROWTH FACTOR; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GROWTH FACTOR; KERATINOCYTE GROWTH FACTOR; MATRIX METALLOPROTEINASE; PROCOLLAGEN C PROTEINASE; PROTEOGLYCAN; TISSUE INHIBITOR OF METALLOPROTEINASE; TRANSFORMING GROWTH FACTOR BETA RECEPTOR 1; VASCULOTROPIN; DIMETHYLMETHYLENE BLUE; DRUG DERIVATIVE; METHYLENE BLUE; PIBENZIMOL; SIGNAL PEPTIDE; SULFUR; THYMIDINE; TRITIUM;

ARTICLE; CELL PROLIFERATION; CELL STIMULATION; CONTROLLED STUDY; DNA SYNTHESIS; GENE EXPRESSION; HUMAN; HUMAN CELL; LOW INTENSITY PULSED ULTRASOUND; NUCLEUS PULPOSUS; PROTEOGLYCAN SYNTHESIS; ULTRASOUND; UPREGULATION; BIOSYNTHESIS; CELL LINE; CYTOLOGY; DNA MICROARRAY; GENE EXPRESSION PROFILING; GENETICS; INTERVERTEBRAL DISK; METABOLISM; POLYMERASE CHAIN REACTION;

BISBENZIMIDAZOLE; CELL LINE; CELL PROLIFERATION; GENE EXPRESSION; GENE EXPRESSION PROFILING; HUMANS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; INTERVERTEBRAL DISK; METHYLENE BLUE; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; POLYMERASE CHAIN REACTION; PROTEOGLYCANS; SULFUR RADIOISOTOPES; THYMIDINE; TRITIUM; ULTRASONICS;

EID: 69849092303     PISSN: 14732262     EISSN: None     Source Type: Journal    
DOI: 10.22203/ecm.v017a02     Document Type: Article

References (24)

1. Alini M, Li W, Markovic P, Aebi, M, Spiro R, Roughley P (2003) The potential and limitations of a cell-seeded collagen/hyaluronan scaffold to engineer an intervertebral disc-like matrix0Spine 28: 973-981.
2. Duarte LR (1983) The stimulation of bone growth by ultrasound. Arch Orthop Trauma Surg 101: 153-159.
3. Fujita N, Imai J, Suzuki T, Yamada M, Ninomiya K, Iwasaki R, Morioka H, Matsumoto M, Chiba K, Watanabe S, Suda T, Toyama Y, Miyamoto T (2008) Vascular endothelial growth factor-A is a survival factor for nucleus pulposus cells in the intervertebral disc. Biochem Biophys Res Commun 372: 367-372.
4. Gebauer D, Mayr E, Orthner E, Ryaby JP (2005) Low-intensity pulsed ultrasound: Effects on nonunions. Ultrasound in Med Biol 31: 1391-1402. (Pubitemid 41434056)
5. Gruber HE, Hanley Jr EN (1998) Analysis of aging and degeneration of the human intervertebral disc. Comparison of surgical specimens with normal controls. Spine 23: 751-757.
6. Iwabuchi S, Ito M, Hata J, Chikanishi T, Azuma Y, Haro H (2005) In vitro evaluation of low-intensity pulsed ultrasound in herniated disc resorption. Biomaterials 26: 7104-7114.
7. Iwashina T, Mochida J, Miyazaki T, Watanabe T, Iwabuchi S, Ando K, Hotta T, Sakai D (2006) Low-intensity pulsed ultrasound stimulates cell proliferation and proteoglycan production in rabbit intervertebral disc cells cultured in alginate. Biomaterials 27: 354-361. (Pubitemid 41457238)
8. Klein RG, Eek BC, O'Neill CW, Elin C, Mooney V, Derby RR (2003) Biochemical injection treatment for discogenic low back pain: a pilot study. Spine J 3: 220-226. (Pubitemid 38351752)
9. Masuda K, Takegami K, An H, Kumano F, Chiba K, Andersson GB, Schmid T, Thonar E (2003) Recombinant osteogenic protein-1 upregulates extracellular matrix metabolism by rabbit annulus fibrosus and nucleus pulposus cells cultured in alginate beads. J Orthop Res 21: 922-930.
10. Mayr E, Frankel V, Ruter A (2000) Ultrasound-An alternative healing method for nonunions? Arch Orthop Trauma 120: 1-8.
11. Miyamoto K, An HS, Sah RL, Akeda K, Okuma M, Otten L, Thonar EJ, Masuda K (2005) Exposure to pulsed low intensity ultrasound stimulates extracellular matrix metabolism of bovine intervertebral disc cells cultured in alginate beads. Spine 30: 2398-2405.
12. Mizuno H, Roy AK, Vacanti CA, Kojima K, Ueda M, Bonassar LJ (2004) Tissue-engineered composites of annulus fibrosus and nucleus pulposus for intervertebral disc replacement. Spine 29: 1290-1297. (Pubitemid 38781631)
13. Nishida K, Kang JD, Suh JK, Robbins PD, Evans CH, Gilbertson LG (1998) Adenovirus-mediated gene transfer to nucleus pulposus cells: implication for the treatment of intervertebral disc degeneration. Spine 23: 2437-2443. (Pubitemid 28536066)
14. Nolte PA, Krans A, Patka P, Janssen IMC, Ryaby JP, Albers GHR (2001) Low-intensity pulsed in the treatment of nonunions. J Trauma 51: 693-702.
15. Paravizi J, Wu CC, Lewallen DG, Greenleaf JF, Bolander ME (1999) Low-intensity ultrasound stimulates proteoglycan synthesis in rat chondrocytes by increasing aggrecan gene expression. J Orthop Res 17: 488-494.
16. Paravizi J, Parpura V, Greenleaf JF, Bolander ME (2002) Calcium signaling is required for ultrasound stimulated aggrecan synthesis by rat chondrocytes. J Orthop Res 20: 51-57. (Pubitemid 34071421)
17. Phillips FM, An H, Kang JD, Boden SD, Weinstein J (2003) Biologic treatment for intervertebral disc degeneration. Spine 28: S99.
18. Sakai D, Mochida J, Yamamoto Y, Toh E, Iwashina T, Miyazaki T, Inokuchi S, Ando K, Hotta T (2004) Immortalization of human nucleus pulposus cells by a recombinant SV40 adenovirus vector: establishment of a novel cell line for the study of human nucleus pulposus cells. Spine 29: 1515-1523. (Pubitemid 38943834)
19. Sakai D, Mochida J, Iwashina T, Hiyama A, Omi H, Imai M, Nakai T, Ando K, Hotta T (2006) Regenerative effects of transplanting mesenchymal stem cells embedded in atelocollagen to the degenerated intervertebral disc. Biomaterials 27: 335-345. (Pubitemid 41457236)
20. Warden SJ, Bennell JM, McMeeken JM, Wark JD (2000) Acceleration of fresh fracture repair using the sonic accelerated fracture healing system (SAFUS): A review. Calcif Tissue Int 66: 157-163.
21. Yoon ST, Su Kim K, Li J, Soo Park J, Akamaru T, Elmer WA, Hutton WC (2003) The effect of bone morphogenetic protein-2 on rat intervertebral disc cells in vitro. Spine 28: 1773-1780. (Pubitemid 37012751)
22. Zhang ZJ, Huckle J, Francomano CA, Spencer RG (2002) The influence of pulsed low-intensity ultrasound on matrix production of chondrocytes at different stages of differentiation: an explant study. Ultrasound Med Biol 28: 1547-1553. (Pubitemid 36020332)
23. Zhang ZJ, Huckle J, Francomano CA, Spencer RG (2003) The effects of pulsed low intensity ultrasound on chondrocyte viability, proliferation, gene expression and matrix production. Ultrasound Med Biol 29: 1645-1651. (Pubitemid 37488075)
24. Zhou S, Schmelz A, Seufferlein T, Li Y, Zhao J, Bachem MG (2004) Molecular mechanisms of low intensity pulsed ultrasound in human skin fibroblasts. J Biol Chem 24: 54463-54469. (Pubitemid 40053186)