High-throughput, cell-free, liposome-based approach for assessing in vitro activity of lipid kinases

Journal of Biomolecular Screening

Volumn 14, Issue 7, 2009, Pages 838-844

  Demian, Douglas J ^a   Clugston, Susan L ^a   Foster, Meta M ^a   Rameh, Lucia ^b   Sarkes, Deborah ^b   Townson, Sharon A ^a   Yang, Lily ^a   Zhang, Melvin ^a   Charlton, Maura E ^a  

^a PFIZER INC   (United States)

^b BOSTON BIOMEDICAL RESEARCH INSTITUTE   (United States)

Author keywords

Fluorescent polarization; Lipid kinase; Liposome; Luminescence; PIP4KII

Indexed keywords

LIPID; LIPOSOME; PHOSPHATIDYLINOSITOL 4 PHOSPHATE KINASE; PHOSPHORUS 32; PHOSPHOTRANSFERASE;

ARTICLE; AUTORADIOGRAPHY; CALORIMETRY; CELL FREE SYSTEM; CONTROLLED STUDY; DOSIMETRY; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; IN VITRO STUDY; NUCLEOTIDE SEQUENCE; PHARMACOPHORE; PRIORITY JOURNAL; THIN LAYER CHROMATOGRAPHY; VALIDITY;

ADENOSINE TRIPHOSPHATE; CALORIMETRY; CELL-FREE SYSTEM; CHROMATOGRAPHY, THIN LAYER; CRYSTALLOGRAPHY, X-RAY; DENSITOMETRY; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; INHIBITORY CONCENTRATION 50; KINETICS; LIPID METABOLISM; LIPOSOMES; PHOSPHORUS RADIOISOTOPES; PHOSPHOTRANSFERASES; PROTEIN KINASE INHIBITORS; REPRODUCIBILITY OF RESULTS; SUBSTRATE SPECIFICITY;

EID: 69549130528     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057109339205     Document Type: Article

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