Partial purification and characterization of five α-amylases from a wheat local variety (Balady) during germination

Australian Journal of Basic and Applied Sciences

Volumn 3, Issue 3, 2009, Pages 1740-1748

  Mohamed, Saleh A ^a,b   Al Malki, Abdulrahman L ^a   Kumosani, Taha A ^a  

^a KING ABDULAZIZ UNIVERSITY   (Saudi Arabia)

^b NATIONAL RESEARCH CENTRE   (Egypt)

Author keywords

amylase; Characterization; Germination; Triticum aestivum; Wheat

Indexed keywords

EID: 69549090671     PISSN: 19918178     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (36)

1. Abe, R., A.Y. Chib, T. Nakajima, 2002. Characterization of the functional module responsible for the low temperature optimum of rice α-amylase (Amy 3E). Biologia Bratislava, 57: 197-202.
2. Adewale, I.O., E.N. Agumanu, F.I. Otith-Okoronkwo, 2006. Comparative studies on α-amylases from malted maize (Zea mays), millet (Eleusine coracana) and sorghum (Sorghum bicolor). Carbohyd polym, 66: 71-74.
3. Agu, R.C., G.H. Palmer, 1997. The effect of temperature on the modification of sorghum and barley during malting. Process Biochem., 32: 501-507.
4. Beers, E., Duke, S., 1990. Characterization of α-amylase from shoots and cotyledons of pea (Pisum sativum L.) seedlings. Plant Physiol., 92: 1154-1163.
5. Berbezy, P., L. Legendre, A. Maujean, 1996. Purification and characterization of α-amylase from vine inter-nodes. Plant Physiol and Biochem., 34: 353-361.
6. Bernfeld, P., 1951. Enzymes of starch degradation and synthesis. Adv. Enzymol., 12: 379-428.
7. Bewly, J.D., M. Black, 1985. Seeds: Physiology of development and germination (pp. 1-33) New York: Plenum Press.
8. Bradford, M.M., 1976. A rapid sensitive method of quantitation micro gram quantities of proteins utilizing the principles of protein-dye binding. Analyt. Biochem., 72: 248-254.
9. Bush, D.S., L. Sticher, R.V. Hwystee, D. Wegner, R.L. Jones, 1989. The calcium requirement for stability and enzymatic activity of two isoforms of barley aleuron α-amylase. J. Biol. Chem., 264: 19392-19398.
10. Elarbi, M.B., H. Khemiri, T. Jridi, J.B. Hamida, 2009. Purification and characterization of α-amylase from safflower (Carthamus tinctorius L.) germinating seeds. C.R. Biologies (In Press).
11. Fahmy, A.S., M.A. Mohamed, T.M. Mohamed, S.A. Mohamed, 2000. Distribution of α-amylase in the Gramineae. Partial purification and characterization of α-amylase from Egyptian cultivar of wheat Triticum aestivium. Bull. NRC, Egypt, 25: 61-80.
12. Fischer, E.H., I. Kohtes, 1951. Purification de l'invertase de levure. Helv. Chim. Acta, 34: 1123- 1131.
13. Georg-Kraemer, J.E., E.C. Mundstock, S. Cavalli-Molina, 2001. developmental expression of amylases during barley malting. J. Cereal Sci., 33: 279-288.
14. Hill, R.D., A.W. McGregor, 1988. Cereal α-amylases in grain research and Technology. In Advances in Cereal Science and Technology (Ed. by Pomeranz, Y.), 9: 217-261. St Paul, USA, AACC.
15. Janeck, S., S. Belaz, 1992. α-amylases and approaches leading to their enhanced stability. FEBS Letters, 304: 1-3.
16. Jones, R.L., J.V. Jacobsen, 1991. Regulation of the synthesis and transport of secreted proteins in cereal aleurone. Internatl. Rev. Cytol., 126: 49-88.
17. Kruger, J.E., B.A. Marchylo, 1985. A comparison of the catalysis of starch components by isoenzymes from the two major groups of germinated wheat alpha-amylase. Cereal Chem., 62: 11-18.
18. Kumar, R.S.S., S.A. Singh, A.G.A. Rao, 2009. Conformational stability of α-amylase from malted sorghum (Sorghum bicolor): reversible unfolding by denaturants. Biochimie, 91: 548-557.
19. MacGregor, A.W., 1977. Isolation, purification and electrophoretic properties of an α-amylase from malted barley. Inst Brew., 83: 100-103.
20. Marchylo, B., J.E. Kruger, G.N. Irvine, 1976. α-Amylase from immature red spring wheat. I- Purification and some chemical and physical properties. Cereal Chem., 53: 157-173.
21. Meredith, P., L.D. Jenkins, 1973. Amylase activity of sproutdamaged, malted and immatured wheat. Cereal Chem., 50: 243-253.
22. Mitchell, E.D., 1972. Homogenous α-amylases from malted barley. Phytochem., 11: 1673-1676.
23. Mitsui, T., J. Yamaguchi, T. Akazawa, 1996. Physicochemical and serological characterization of rice alpha-amylase isoforms and identification of their corresponding genes. Plant Physiol., 110: 1395-1404.
24. Muralikrishna, G., M. Nirmala, 2005. Cereal α-amylases-an overview. Carbohydr. Polym, 60: 163-173.
25. Nirmala, M., G. Muralikrishna, 2003a. Three α-amylases from malted finger millet (Ragi, Eleusine coracana, Indaf-15)-purification and partial characterization. Phytochem., 62: 21-30.
26. Nirmala, M., G. Muralikrishna, 2003b. Properties of three purified α-amylases from malted finger millet (Ragi, Eleusine coracana, Indaf-15). Carbohydr. Polym., 54: 43-50.
27. Noman, A.S.M., M.A. Hoque, P.K. Sen, M.R. Karim, 2006. Purification and some properties of α-amylase from post-harvest Pachyrhizus erosus L. tuber. Food Chem., 99: 444-449.
28. Okamoto, K., T. Akazawa, 1979. Enzymic mechanisms of starch breakdown in germinating rice seeds 7. Amylase formation in the epithelium. Plant Physiol., 64: 337-340.
29. Okamoto, K., H. Kitano, T. Akazawa, 1980. Biosynthesis and excretion of hydrolases in germinating cereal seeds. Plant Cell Physiol., 21: 201-204.
30. Parkin, K.L., 1993. In: Food Processing, third ed. T. Nagodawithana and Reed. V.G. (Eds). Academic Press, New York, 7-36.
31. Sprinz, C., 1999. In: Food Chemistry. Belitz, H.D.; Gross, W.; Eds. Springer Verlag, Berlin, Heidelberg, New York, 92-151.
32. Subbarao, K.V., R. Datta, R. Sharma, 1998. Amylases synthesis in scutellum and aleurone layer of maize seeds. Phytochem., 49: 657-666.
33. Tkachuk, R., J.E. Kruger, 1974. Wheat α-amylases II. Physical characterization. Cereal Chem., 51: 508-529.
34. Tripathi, P., L.L. Leggio, Johanna J. Mansfeld, R. Ulbrich-Hofmann, A.M. Kayastha, 2007. α-amylase from mung beans (Vigna radiata) - Correlation of biochemical properties and tertiary structure by homology modeling. Phytochem., 68: 1623-1631.
35. Vihinen, M., P. Mantsala, 1989. Microbial amylolytic enzymes. Crit Rev Biochem Molec Biol., 24: 329-348.
36. Warchaleswiski, J.R., R. Tkachuk, 1978. Durum wheat α-amylases. Isolation and purification. Cereal Chem., 55: 146-156.