An optimized procedure for detection of proteins on carrier ampholyte isoelectric focusing and immobilized pH gradient gels with imidazole and zinc salts: Its application to the identification of isoelectric focusing separated isoforms by in-gel proteolysis and mass spectrometry analysis

Electrophoresis

Volumn 22, Issue 9, 2001, Pages 1677-1685

  Castellanos Serra, Lila ^a   Vallin, Antonio ^b   Proenza, Wilfredo ^a   Le Caer, Jean Pierre ^c   Rossier, Jean ^c  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

^b CENTER OF MOLECULAR IMMUNOLOGY   (Cuba)

^c Brain Plasticity Unit   (France)

Author keywords

Imidazole zinc staining; In gel digestion; Isoelectric focusing; Mass spectrometry; Protein staining

Indexed keywords

CHELATION; ELECTROPHORESIS; FOCUSING; GELS; MASS SPECTROMETRY; PH; SALTS; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS; ZINC;

CARRIER AMPHOLYTES; IMIDAZOL; IMIDAZOLE-ZINC STAINING; INGEL DIGESTION; ISOELECTRIC FOCUSING; ISOFORMS; PH GRADIENTS; PROTEIN STAINING; SODIUM DODECYL SULPHATE; ZINC SALTS;

PROTEINS;

EID: 69449102471     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/1522-2683(200105)22:9<1677::AID-ELPS1677>3.0.CO;2-H     Document Type: Article

References (15)

1. Castellanos-Serra, L., Hardy, E., Proenza, W., Huerta H., Gonzalez, L. J., Le Caer, J. P., Moritz, R., Simpson, R, in: Kamp, R. M., Kyriakidis, O., Choli-Papadodlulou, T. (Eds.), Proteins and Proteome Analysis, Springer, Heidelberg 1999, pp. 29-52.
2. Castellanos-Serra, L., Hardy, E., Electrophoresis 2001, 22, 864-873.
3. Castellanos-Serra, L., Proenza, W., Huerta, V., Moritz, R. L., Simpson, R. J. Electrophoresis 1999, 20, 732-737.
4. Chen, P., Hussain, A., Tai, H. H., Anal. Biochem. 1994, 219, 159-161.
5. Westermeier, R., Electrophorese Praktikum, VCH,Weinheim. 1990, pp. 159-160.
6. Goerg, A. Two-Dimensional Protein Electrophoresis Using Immobilized pH Gradients. A Laboratory Manual, Technical University of Munich, Weihenstephan 1997.
7. Robertson, E. F., Danielly, K., Malloy, P. I., Reeves, H., Anal. Biochem. 1987, 167, 290-294.
8. Laemmli, U. K., Nature, 1970, 27, 680-685.
9. Fernandez-Patron, C., Calero, M., Rodriguez, P., Garcia, J., Madrazo, J., Musacchio, A., Estrada, R., Frank, R., Castellanos-Serra, L., Mendez, E., Anal. Biochem. 1995, 224, 203-211.
10. Fernandez-Patron, C., Castellanos-Serra, L., Hardy, E., Guerra, M., Estevez, E., Mehl, E., Frank, R. W., Electrophoresis 1998, 19, 2398-2406.
11. Shevchenko, A., Wilm, M., Vorm, O., Mann, M., Anal. Chem. 1996, 68, 850-858.
12. Ogorzaiek, R. R., Stevenson, T. I., Mitchell, C., Loo, J. A., Andrews, P. C., Anal. Chem. 1996, 68, 1910-1917.
13. Garrels, I., J. Biol. Chem. 1989, 264, 5269-5282.
14. Courchesne, P. L., Patterson, S. D., in Link, A. (Ed.), Methods in Molecular Biology, Vol. 112: 2-D Proteome Analysis Protocols, Humana Press, Totowa, NJ 1999, pp. 487-511.
15. Steinberg, T., Chemokalskaya, E., Berggren, K., López, M., Diwu, Z., Haugland, R., Patton, W., Electrophoresis 2000, 21, 486-496.