Enhanced flavonoid production in hairy root cultures of Glycyrrhiza uralensis Fisch by combining the over-expression of chalcone isomerase gene with the elicitation treatment

Plant Cell Reports

Volumn 28, Issue 8, 2009, Pages 1205-1213

  Zhang, Hai Chao ^a   Liu, Jing Mei ^b   Lu, Hong Yu ^c   Gao, Shan Lin ^a  

^a CHINA PHARMACEUTICAL UNIVERSITY   (China)

^b National Engineering Research Center for Crop Molecular Design   (China)

^c GUANGDONG OCEAN UNIVERSITY   (China)

Author keywords

Chalcone isomerase(chi); Elicitor; Flavonoids; Genetic transformation; Glycyrrhiza uralensis Fisch; Hairy roots

Indexed keywords

AGROBACTERIUM RHIZOGENES; GLYCYRRHIZA; GLYCYRRHIZA URALENSIS;

CHALCONE ISOMERASE; COMPLEMENTARY DNA; FLAVONOID; ISOMERASE; PLANT DNA; VEGETABLE PROTEIN;

ARTICLE; BIOSYNTHESIS; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETIC TRANSFORMATION; GENETICS; GLYCYRRHIZA URALENSIS; METABOLISM; PLANT GENE; PLANT ROOT; RHIZOBIUM RADIOBACTER; TRANSGENIC PLANT;

DNA, COMPLEMENTARY; DNA, PLANT; FLAVONOIDS; GENE EXPRESSION REGULATION, PLANT; GENES, PLANT; GLYCYRRHIZA URALENSIS; INTRAMOLECULAR LYASES; PLANT PROTEINS; PLANT ROOTS; PLANTS, GENETICALLY MODIFIED; RHIZOBIUM RADIOBACTER; TRANSFORMATION, GENETIC;

EID: 69449097014     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00299-009-0721-3     Document Type: Article

References (41)

1. Alpizar E, Dechamp E, Espeout S, Royer M, Lecouls AC, Nicole M, Bertrand B, Lashermes P, Etienne H (2006) Efficient production of Agrobacterium rhizogenes-transformed roots and composite plants for studying gene expression in coffee roots. Plant Cell Rep 25:959-967.
2. Ashraf M (2009) Biotechnological approach of improving plant salt tolerance using antioxidants as markers. Biotechnol Adv 27:84-93.
3. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1995) Short protocols in molecular biology. Wiley, New York.
4. Ayabe SI, Iida K, Furuya T (1986) Studies on plant-tissue culture.46. Stress-induced formation of Echinatin and a metabolite, 5′-Prenyl-Licodione, in cultured Glycyrrhiza-Echinata cells. Phytochemistry 25:2803-2806.
5. Bednarek P, Kerhoas L, Einhorn J, Franski R, Wojtaszek P, Rybus-Zajac M, Stobiecki M (2003) Profiling of flavonoid conjugates in Lupinus albus and Lupinus angustifolius responding to biotic and abiotic stimuli. J Chem Ecol 29:1127-1142.
6. Bourgaud F, Bouque V, Guckert A (1999) Production of flavonoids by Psoralea hairy root cultures. Plant Cell Tissue Organ Cult 56:96-103.
7. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254.
8. Cain CC, Saslowsky DE, Walker RA, Shirley BW (1997) Expression of chalcone synthase and chalcone isomerase proteins in Arabidopsis seedlings. Plant Mol Biol 35:377-381.
9. Damgaard O, Rasmussen O (1991) Direct regeneration of transformed shoots in Brassica napus from hypocotyl infections with Agrobacterium rhizogenes. Plant Mol Biol 17:1-8.
10. Doolittle WF (1987) The origin and function of intervening sequences in DNA: a review. Am Nat 130:915-928.
11. Fukai T, Marumo A, Kaitou K, Kanda T, Terada S, Nomura T (2002) Antimicrobial activity of licorice flavonoids against methicillin-resistant Staphylococcus aureus. Fitoterapia 73:536-539.
12. Guan JY, Wang WW, Ma MT, Zhang SY, Xing Y (1995) Investigation on maceration extract preparation of Saussurea involuerata. J Shen yang Pharma Univ 12:209-211.
13. Jez JM, Noel JP (2002) Reaction mechanism of chalcone isomerase. pH dependence, diffusion control, and product binding differences. J Biol Chem 277:1361-1369.
14. Jouhikainen K, Lindgren L, Jokelainen T, Hiltunen R, Teeri TH, Oksman-Caldentey KM (1999) Enhancement of scopolamine production in Hyoscyamus muticus L. hairy root cultures by genetic engineering. Planta 208:545-551.
15. Kang HJ, Anbazhagan VR, You XL, Moon HK, Yi JS, Choi YE (2006) Production of transgenic Aralia elata regenerated from Agrobacterium rhizogenes-mediated transformed roots. Plant Cell Tissue Organ Cult 85:187-196.
16. Kimura Y, Aoki T, Ayabe S (2001) Chalcone isomerase isozymes with different substrate specificities towards 6′-hydroxy- and 6′-deoxychalcones in cultured cells of Glycyrrhiza echinata, a leguminous plant producing 5-deoxyflavonoids. Plant Cell Physiol 42:1169-1173.
17. Li W, Asada Y, Yoshikawa T (2000) Flavonoid constituents from Glycyrrhiza glabra hairy root cultures. Phytochemistry 55:447-456.
18. Li W, Koike K, Asada Y, Hirotani M, Rui H, Yoshikawa T, Nikaido T (2002) Flavonoids from Glycyrrhiza pallidiflora hairy root cultures. Phytochemistry 60:351-355.
19. Li M, Wang GX, Lin JS (2004) Calcium stimulates the adaptation of cultured liquorice cells to PEG-induced water stress. Russ J Plant Physiol 51:518-524.
20. Li FX, Jin ZP, Zhao DX, Cheng LQ, Fu CX, Ma F (2006) Overexpression of the Saussurea medusa chalcone isomerase gene in S. involucrata hairy root cultures enhances their biosynthesis of apigenin. Phytochemistry 67:553-560.
21. Lister CE, Lancaster JE, Walker JRL (1996) Developmental changes in enzymes of flavonoid biosynthesis in the skins of red and green apple cultivars. J Sci Food Agric 71:313-320.
22. Liu C, Wang Y, Xu X, Ouyang F, Ye H, Li G (1999) Improvement of artemisinin accumulation in hairy root culture of Artemisia annua L. by fungal elicitor. Bioprocess Biosyst Eng 20:161-164.
23. Liu B, Beuerle T, Klundt T, Beerhues L (2004) Biphenyl synthase from yeast-extract-treated cell cultures of Sorbus aucuparia. Planta 218:492-496.
24. Mehdy MC, Lamb CJ (1987) Chalcone isomerase cDNA cloning and mRNA induction by fungal elicitor, wounding and infection. EMBO J 6:1527-1533.
25. Moustafa E, Wong E (1967) Purification and properties of chalcone flavanone isomerase from soybean seed. Phytochemistry 6:625-632.
26. Moyano E, Jouhikainen K, Tammela P, Palazon J, Cusido RM, Pinol MT, Teeri TH, Oksman-Caldentey KM (2003) Effect of pmt gene overexpression on tropane alkaloid production in transformed root cultures of Datura metel and Hyoscyamus muticus. J Exp Bot 54:203-211.
27. Muir SR, Collins GJ, Robinson S, Hughes S, Bovy A, Ric De Vos CH, van Tunen AJ, Verhoeyen ME (2001) Overexpression of petunia chalcone isomerase in tomato results in fruit containing increased levels of flavonols. Nat Biotechnol 19:470-474.
28. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497.
29. Nakamura K, Akashi T, Aoki T, Kawaguchi K, Ayabe S (1999) Induction of isoflavonoid and retrochalcone branches of the flavonoid pathway in cultured Glycyrrhiza echinata cells treated with yeast extract. Biosci Biotechnol Biochem 63:1618-1620.
30. Palazon J, Moyano E, Cusido RM, Bonfill M, Oksman-Caldentey KM, Pinol MT (2003) Alkaloid production in Duboisia hybrid hairy roots and plants overexpressing the h6h gene. Plant Sci 165:1289-1295.
31. Pollak PE, Vogt T, Mo Y, Taylor LP (1993) Chalcone synthase and flavonol accumulation in stigmas and anthers of Petunia hybrida. Plant Physiol 102:925-932.
32. Ralston L, Subramanian S, Matsuno M, Yu O (2005) Partial reconstruction of flavonoid and isoflavonoid biosynthesis in yeast using soybean type I and type II chalcone isomerases. Plant Physiol 137:1375-1388.
33. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York.
34. Shi HP, Kintzios S (2003) Genetic transformation of Pueraria phaseoloides with Agrobacterium rhizogenes and puerarin production in hairy roots. Plant Cell Rep 21:1103-1107.
35. Shimada N, Aoki T, Sato S, Nakamura Y, Tabata S, Ayabe S (2003) A cluster of genes encodes the two types of chalcone isomerase involved in the biosynthesis of general flavonoids and legume-specific 5-deoxy (iso) flavonoids in Lotus japonicus. Plant Physiol 131:941-951.
36. Shul'ts EE, Petrova TN, Shakirov MM, Chernyak EI, Tolstikov GA (2000) Flavonoids of roots of Glycyrrhiza uralensis growing in Siberia. Chem Nat Compd 36:362-368.
37. Toivonen L, Rosenqvist H (1995) Establishment and growth-characteristics of Glycyrrhiza-Glabra hairy root cultures. Plant Cell Tissue Organ Cult 41:249-258.
38. Yang DC, Choi YE (2000) Production of transgenic plants via Agrobacterium rhizogenes-mediated transformation of Panax ginseng. Plant Cell Rep 19:491-496.
39. Yang SH, Liu XF, Shen X, Zheng JH (2006) Ri plasmid transformation of Glycyrrhiza uralensis and effects of some factors on growth of hairy roots. Zhongguo Zhong Yao Za Zhi 31:875-878.
40. Zhang L, Ding R, Chai Y, Bonfill M, Moyano E, Oksman-Caldentey KM, Xu T, Pi Y, Wang Z, Zhang H, Kai G, Liao Z, Sun X, Tang K (2004) Engineering tropane biosynthetic pathway in Hyoscyamus niger hairy root cultures. Proc Natl Acad Sci USA 101:6786-6791.
41. Zhang L, Yang B, Lu B, Kai G, Wang Z, Xia Y, Ding R, Zhang H, Sun X, Chen W, Tang K (2007) Tropane alkaloids production in transgenic Hyoscyamus niger hairy root cultures over-expressing putrescine N-methyltransferase is methyl jasmonate-dependent. Planta 225:887-896.