Mixed-substrate (glycerol tributyrate and fibrin) zymography for simultaneous detection of lipolytic and proteolytic enzymes on a single gel

Electrophoresis

Volumn 30, Issue 12, 2009, Pages 2234-2237

  Choi, Nack Shick ^a   Choi, Jong Hyun ^a   Kim, Bo Hye ^a   Han, Yun Jon ^a   Kim, Joong Su ^a   Lee, Seung Goo ^b   Song, Jae Jun ^a  

^a Jeonbuk Branch Institute   (South Korea)

^b Systems Microbiology Research Center   (South Korea)

Author keywords

Electrotransfer; Lipase; Mixed substrates; Protease; Zymography

Indexed keywords

BACTERIOLOGY; ELECTROPHORESIS; ENZYME ACTIVITY; SUBSTRATES;

ELECTROTRANSFER; ENZYMATIC ACTIVITIES; LIPOLYTIC; LIPOLYTIC ENZYMES; MIXED SUBSTRATES; NATIVE CONFORMATION; PROTEASE; PROTEOLYTIC ENZYME; SIMULTANEOUS DETECTION; ZYMOGRAPHY;

GLYCEROL;

ANAZOLENE SODIUM; BACTERIAL ENZYME; DODECYL SULFATE SODIUM; FIBRIN; PROTEINASE; TRIACYLGLYCEROL LIPASE; TRIBUTYRIN; TRITON X 100;

ANALYTIC METHOD; ARTICLE; BACILLUS; BACILLUS LICHENIFORMIS; BACTERIAL STRAIN; BACTERIUM ISOLATION; CONTROLLED STUDY; ENZYMATIC ASSAY; ENZYME ACTIVITY; ENZYME SUBSTRATE; GEL ELECTROPHORESIS; INCUBATION TIME; LIPOLYSIS; MASS SPECTROMETRY; MOLECULAR IMAGING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DEGRADATION; PROTEIN TRANSPORT; SOIL MICROFLORA; STAINING; STAPHYLOCOCCUS; STAPHYLOCOCCUS PASTEURI; ZYMOGRAPHY;

EID: 69449094890     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200800727     Document Type: Article

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