Biochemical analysis of oligomerization of expanded polyalanine repeat proteins

Journal of Neuroscience Research

Volumn 87, Issue 10, 2009, Pages 2290-2296

  Nojima, Jun ^a   Oma, Yoko ^a   Futai, Eugene ^a   Sasagawa, Noboru ^a   Kuroda, Reiko ^a   Turk, Boris ^b   Ishiura, Shoichi ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b JO EF STEFAN INSTITUTE   (Slovenia)

Author keywords

Oligomerization; Polyalanine diseases; Sucrose density centrifugation; Trypsin digestion

Indexed keywords

GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; POLYALANINE REPEAT PROTEIN; POLYPEPTIDE; UNCLASSIFIED DRUG; YELLOW FLUORESCENT PROTEIN; ALANINE; BACTERIAL PROTEIN; PEPTIDE; PHOTOPROTEIN; POLYALANINE; TRYPSIN; YELLOW FLUORESCENT PROTEIN, BACTERIA;

ANIMAL CELL; ARTICLE; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; NONHUMAN; OLIGOMERIZATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; SUCROSE DENSITY GRADIENT CENTRIFUGATION; AMINO ACID SEQUENCE; ANALYSIS OF VARIANCE; ANIMAL; CELL STRAIN COS1; CERCOPITHECUS; CHEMISTRY; GENETIC TRANSFECTION; GENETICS; HAMSTER; HUMAN; METABOLISM; METHODOLOGY; PROTEIN CONFORMATION; TWO HYBRID SYSTEM;

ALANINE; ANALYSIS OF VARIANCE; ANIMALS; BACTERIAL PROTEINS; CERCOPITHECUS AETHIOPS; COS CELLS; CRICETINAE; HUMANS; LUMINESCENT PROTEINS; PEPTIDES; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS; REPETITIVE SEQUENCES, AMINO ACID; TRANSFECTION; TRYPSIN; TWO-HYBRID SYSTEM TECHNIQUES;

EID: 69249202639     PISSN: 03604012     EISSN: 10974547     Source Type: Journal    
DOI: 10.1002/jnr.22052     Document Type: Article

References (20)

1. Abu-Baker A, Messaed C, Laganiere J, Gaspar C, Brais B, Rouleau GA. 2003. Involvement of the ubiquitin-proteasome pathway and molecular chaperones in oculopharyngeal muscular dystrophy. Hum Mol Genet 12:2609-2623.
2. Alba MM, Guigo R. 2004. Comparative analyis of amino acid repeats in rodents and humans. Genome Res 14:549-554.
3. Albrecht A, Mundlos S. 2005. The other trinucleotide repeat: polyalanine expansion disorders. Curr Opin Genet Dev 15:285-293.
4. Albrecht AN, Kornak U, Boddrich A, Suring K, Robinson PN, Stiege AC, Lurz R, Stricker S, Wanker EE, Mundlos S. 2004. A molecular pathogenesis for transcription factor associated poly-alanine tract expansions. Hum Mol Genet 13:2351-2359.
5. Becher MW, Kotzuk JA, Davis LE, Bear DG. 2000. Intranuclear inclusions in oculopharyngeal muscular dystrophy contain poly(A) binding protein 2. Ann Neurol 48:812-815.
6. Dehay B, Bertolotti A. 2006. Critical role of the proline-rich region in huntingtin for aggregation and cytotoxicity in yeast. J Biol Chem 281:35608-35615.
7. Giri K, Bhattacharyya NP, Basak S. 2007. pH-dependent self-assembly of polyalanine peptides. Biophys J 92:293-302.
8. Heiser V, Scherzinger E, Boeddrich A, Nordhoff E, Lurz R, Schugardt N, Lehrach H, Wanker EE. 2000. Inhibition of huntingtin fibrillogenesis by specific antibodies and small molecules: implications for Huntington's disease therapy. Proc Natl Acad Sci USA 97:6739-6744.
9. Nagai Y, Tucker T, Ren H, Kenan DJ, Henderson BS, Keene JD, Strittmatter WJ, Burke JR. 2000. Inhibition of polyglutamine protein aggregation and cell death by novel peptides identified by phage display screening. J Biol Chem 275:10437-10442.
10. Nagai Y, Inui T, Popiel HA, Fujikake N, Hasegawa K, Urade Y, Goto Y, Naiki H, Toda T. 2007. A toxic monomeric conformer of the polyglutamine protein. Nat Struct Mol Biol 14:332-340.
11. Oma Y, Kino Y, Sasagawa N, Ishiura S. 2004. Intracellular localization of homopolymeric amino acid-containing proteins expressed in mammalian cells. J Biol Chem 279:21217-21222.
12. Oma Y, Kino Y, Toriumi K, Sasagawa N, Ishiura S. 2007. Interactions between homopolymeric amino acids (HPAAs). Prot Sci 16:2195-2204.
13. Paul S. 2007. Polyglutamine-mediated neurodegeneration: use of chaperones as prevention strategy. Biochemistry 72:359-366.
14. Rankin J, Wyttenbach A, Rubinsztein DC. 2000. Intracellular green fluorescent protein-polyalanine aggregates are associated with cell death. Biochem J 348:15-19.
15. Shanmugam V, Dion P, Rochefort D, Laganiere J, Brais B, Rouleau GA. 2000. PABP2 polyalanine tract expansion causes intranuclear inclusions in oculopharyngeal muscular dystrophy. Ann Neurol 48:798-802.
16. Sharipo A, Imreh M, Leonchiks A, Brändén C, Masucci MG. 2001. cis-Inhibition of proteasomal degradation by viral repeats: impact of length and amino acid composition. FEBS Lett 499:137-142.
17. Shinchuk LM, Sharma D, Blondelle SE, Reixach N, Inouye H, Kirschner DA. 2005. Poly-(L-alanine) expansions form core beta-sheets that nucleate amyloid assembly. Proteins 61:579-589.
18. Takahashi Y, Okamoto Y, Popiel HA, Fujikake N, Toda T, Kinjo M, Nagai Y. 2007. Detection of polyglutamine protein oligomers in cells by fluorescence correlation spectroscopy. J Biol Chem 282:24039 24048.
19. Toriumi K, Oma Y, Kino Y, Futai E, Sasagawa N, Ishiura S. 2008. Expression of polyalanine stretches induces mitochondrial dysfunction. J Neurosci Res 86:1529-1537.
20. Wang Q, Mosser DD, Bag J. 2005. Induction of HSP70 expression and recruitment of HSC70 and HSP70 in the nucleus reduce aggregation of a polyalanine expansion mutant of PABPN1 in HeLa cells. Hum Mol Genet 14:3673-3684.