The investigation of nematocidal activity in Stenotrophomonas maltophilia G2 and characterization of a novel virulence serine protease

Canadian Journal of Microbiology

Volumn 55, Issue 8, 2009, Pages 934-942

  Huang, Xiaowei ^a   Liu, Junwei ^a   Ding, Junmei ^a   He, Qiusheng ^a   Xiong, Rui ^b   Zhang, Keqin ^a  

^a YUNNAN UNIVERSITY   (China)

^b CHINA PHARMACEUTICAL UNIVERSITY   (China)

Author keywords

Nematocidal activity; Pathogenic factor; Serine protease; Stenotrophomonas maltophilia

Indexed keywords

A PLANTS; BACTERIAL CULTURES; BURSAPHELENCHUS XYLOPHILUS; EXTRACELLULAR PROTEASE; EXTRACELLULAR PROTEINS; FREE-LIVING NEMATODES; GRAM-NEGATIVE BACTERIA; N-TERMINALS; NEMATOCIDAL ACTIVITY; PATHOGENIC FACTOR; PROTEOLYTIC ACTIVITIES; SERINE PROTEASE; SOIL SAMPLE; STENOTROPHOMONAS MALTOPHILIA; VIRULENCE FACTORS;

AMINES; CHROMATOGRAPHIC ANALYSIS; ENZYME ACTIVITY; ORGANIC ACIDS; PURIFICATION;

AMINO ACIDS;

AMINO ACID; CELL PROTEIN; SERINE PROTEINASE; VIRULENCE FACTOR;

AMINO ACID; BACTERIUM; BIOCONTROL AGENT; CHROMATOGRAPHY; CUTICLE; ENZYME ACTIVITY; INHIBITOR; METABOLITE; MOLECULAR ANALYSIS; NEMATODE; PATHOGENICITY; SOIL MICROORGANISM; VIRULENCE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIUM CULTURE; BACTERIUM ISOLATION; CHROMATOGRAPHY; CUTICLE; GRAM NEGATIVE BACTERIUM; METABOLITE; NEMATODE; NONHUMAN; NUCLEOTIDE SEQUENCE; PANAGRELLUS REDIVIVUS; PATHOGENICITY; PLANT; PRIORITY JOURNAL; PROTEIN DEGRADATION; SEQUENCE ANALYSIS; SOIL; STENOTROPHOMONAS MALTOPHILIA; VIRULENCE;

AMINO ACID SEQUENCE; ANIMALS; DNA, BACTERIAL; DNA, RIBOSOMAL; PHYLOGENY; RHABDITIDA; SERINE PROTEASES; STENOTROPHOMONAS MALTOPHILIA; SUBSTRATE SPECIFICITY; VIRULENCE; VIRULENCE FACTORS;

BACTERIA (MICROORGANISMS); BURSAPHELENCHUS XYLOPHILUS; NEGIBACTERIA; NEMATODA; PANAGRELLUS REDIVIVUS; STENOTROPHOMONAS MALTOPHILIA;

EID: 69249134135     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/W09-045     Document Type: Article

References (38)

1. Åhman, J., Johansson, T., Olsson, M., Punt, P.J., van den Hondel, C.A.M.J.J., and Tunlid, A.S. 2002. Improving the pathogenicity of a nematode-trapping fungus by genetic engineering of a subtilisin with nematotoxic activity. Appl. Environ. Microbiol. 689: 3408-3415. doi:10.1128/AEM.68.7.3408- 3415.2002.
2. Anke, H., and Sterner, O. 1997. Nematocidal metabolites from higher fungi. Curr. Org. Chem. 1: 361-374.
3. Barker, K.R. 1998.Introduction and synopsis of advancements in nematology. In Plant and nematode interactions. Edited by K.R. Barker, G.A. Pederson, and G.L. Windham. American Society of Agronomy Inc., Crop Science Society of America Inc., and Soil Science Society of America Inc., Madison, Wis. pp. 1-20.
4. Cox, G. N., Kusch, M., and Edgar, R. S. 1981. Cuticle of Caenorhabditis elegans: its isolation and partial characterization. J. Cell Biol. 90: 7-17. doi:10.1083/jcb.90.1.7. PMID:7251677.
5. Crossman, L.C., Gould, V.C., Dow, J.M., Vernikos, G.S., Okazaki, A., Sebaihia, M. et al. 2008. The complete genome, comparative and functional analysis of Stenotrophomonas maltophilia reveals an organism heavily shielded by drug resistance determinants. Genome Biol. 9: R74. doi:10.1186/gb-2008-9-4-r74. PMID:18419807.
6. Denton, M., and Kerr, K.G. 1998. Microbiological and clinical aspects of infection associated with Stenotrophomonas maltophilia. Clin. Microbiol. Rev. 11: 7-80.
7. Dong, J.Y., Zhao, Z.X., Cai, L., Liu, S.Q., Zhang, H.R., Duan, M., and Zhang, K.Q. 2004. Nematicidal effect of freshwater fungal cultures against the pine-wood nematode, Bursaphelenchus xylophilus. Fungal Divers. 15: 125-135.
8. Dunne, C., Crowley, J.J., Moënne-Loccoz, Y., Dowling, D.N., de Bruijn, F.J., and O'Gara, F. 1997. Bilogical control of Pythimu ultimum by Stenotrophomonas maltophilia W81 is mediated by an extracellular proteolytic activity. Microbiology, 143: 3921-3931. doi:10.1099/00221287-143-12-3921.
9. Dunne, C., Moenne-Loccoz, Y., McCarthy, J., Higgins, P., Powell, J., Dowling, D.N., and O'Gara, F. 1998. Combining proteolytic and phloroglucinol-producing bacteria for improved biocontrol of Pythium-mediated damping-off of sugar beet. Plant Pathol. 47: 299-307. doi:10.1046/j.1365-3059. 1998.00233.x.
10. Dunne, C., Moenne-Loccoz, Y., de Bruijn, F.J., and O'Gara, F. 2000. Overproduction of an inducible extracelluar serine protease improves biological control of Pythium ultimum by Stenotrophomonas maltophilia strain W81. Microbiology, 146: 2069-2078. PMID:10931911.
11. Giesler, L.J., and Yuen, G.Y. 1998. Evalluation of Stenotrophomonas maltophilia strain C3 for biocontrol of brown patch disease. Crop Prot. 17: 509-513. doi:10.1016/S0261-2194(98)00049-0.
12. Griffitts, J.S., Whitacre, J.L., Stevens, D.E., and Aroian, R.V. 2001. Bt toxin resistance from loss of a putative carbohydrate-modifying enzyme. Science, 293: 860-864. doi:10.1126/science.1062441. PMID:11486087.
13. Hauben, L., Vauterin, L., Moore, E.R.B., Hoste, M., and Swings, J. 1999. Genomic diversity of the genus Stenotrophomonas. Int. J. Syst. Bacteriol. 49: 1749-1760. PMID:10555357.
14. Huang, X.W., Zhao, N.H., and Zhang, K.Q. 2004. Extracellular enzymes serving as virulence factors in nematophageous fungi involved in infection of the host. Res. Microbiol. 155: 811-816. doi:10.1016/j.resmic.2004.07.003. PMID:15567274.
15. Huang, X.W., Tian, B.Y., Niu, Q.H., and Zhang, K.Q. 2005. An extracellular protease from Brevibacillus laterosporus G4 without parasporal crystal serve as pathogenetic factor in the infection of nematodes. Res. Microbiol. 156: 719-727. doi:10.1016/j.resmic.2005.02.006. PMID:15950127.
16. Jansson, H.B., and Nordbring-Hertz, B. 1988. Infection mechanisms in the fungus-nematode system. In Diseases of nematodes. Vol.2. Edited by G.O. Poinar Jr. and H.B. Jansson. CRC Press, Boca Raton, Fla. pp. 59-72.
17. Li, J., Yang, J.K., Huang, X.W., and Zhang, K.Q. 2006. Purification and characterization of an extracellular serine protease from Clonostachys rosea and its potential as a pathogenic factor. Process Biochem. 52: 130-139.
18. Lian, L.H., Tian, B.Y., Xiong, R., Zhu, M.Z., Xu, J., and Zhang, K.Q. 2007. Proteases from Bacillus: a new insight into the mechanism of action for rhizobacterial suppression of nematode. Lett. Appl. Microbiol. 45: 262-269. doi:10.1111/j.1472-765X.2007.02184.x. PMID:17718837.
19. Lugtenberg, B.J.J., and Dekker, L.C. 1999. What makes Pseudomonas bacteria rhizosphere competent? Environ. Microbiol. 1: 9-15. doi:10.1046/j.1462-2920.1999.00005.x. PMID:11207713.
20. Luo, H., Li, X., Li, G.H., Pan, Y.B., and Zhang, K.Q. 2006. Acanthocytes of Stropharia rugosoannulata function as a nematode-attacking device. Appl. Environ. Microbiol. 72: 2982-2987. doi:10.1128/AEM.72.4.2982-2987.2006. PMID:16598005.
21. Luo, H., Liu, Y.J., Fang, L., Li, X., Tang, N.H., and Zhang, K.Q. 2007. Coprinus comatus damages nematode buticle mechanically with spiny balls and produces potent toxins to immobilized nematodes. Appl. Environ. Microbiol. 73: 3916-3923. doi:10.1128/AEM.02770-06. PMID:17449690.
22. Matsumoto, K. 2004. Role of bacterial proteases in pseudomonal and serratial keratitis. Biol. Chem. 385: 1007-1016. doi:10.1515/BC.2004.131.
23. Minkwitz, A., and Berg, G. 2001. Comparison of antifungal activites and 16S ribosomal DNA sequences of clinical and environmental isolates of Stenotrophomonas maltophilia. J. Clin. Microbiol. 39: 139-145. doi:10.1128/JCM.39.1.139-145.2001. PMID:11136762.
24. Miyaji, T., Otta, Y., Shibata, T., Mitsui, K., Nakagawa, T., Watanabe, T., et al. 2005. Purification and characterization of extracellular alkaline serine protease from Stenotrophomonas maltophilia strain S-1. Lett. Appl. Microbiol. 41: 253-257. doi:10.1111/j.1472-765X.2005.01750.x. PMID:16108916.
25. Niu, Q.H., Huang, X.W., Tian, B.Y., Yang, J.K., Liu, J., Zhang, L., and Zhang, K.Q. 2006a. Bacillus sp. B16 kills nematodes with a serine protease identified as a pathogenic factor. Appl. Microbiol. Biotechnol. 69: 722-730. doi:10.1007/s00253-005-0019-5. PMID:16025329.
26. Niu, Q.H., Huang, X.W., Zhang, L., Li, Y.X., Li, J., Yang, J.K., and Zhang, K.Q. 2006b. A neutral protease from Bacillus nematocida, another potential virulence factor in the infection against nematodes. Arch. Microbiol. 185: 439-448. doi:10.1007/s00203-006-0112-x. PMID:16775750.
27. Tian, B., Yang, J., Lian, L., Wang, C., and Zhang, K.Q. 2007. Role of neutral protease from Brevibacillus laterosporus in pathogenesis of nematode. Appl. Microbiol. Biotechnol. 74: 372-380. doi:10.1007/s00253-006-0690-1. PMID:17109172.
28. Tunlid A., Rosen, S., Ek, B., and Rask, L. 1994. Purification and characterization of an extracellular serine protease from the nematode-trapping fungus Arthrobotrys oligospora. Microbiology, 140: 1687-1695. doi:10.1099/13500872-140-7-1687. PMID:8075805. (Pubitemid 24231157)
29. Van Den Mooter, M., and Swings, J. 1990. Numerical analysis of 295 phenotypic features of 266 Xanthomonas strains and related strains and animproved taxonomy of the genus. Int. J. Syst. Bacteriol. 40: 348-369. PMID:2275852.
30. Wang, M., Yang, J.K., and Zhang, K.Q. 2006. Characterization of an extracellular protease and its cDNA from the nematode-trapping fungus Monacrosporium microscaphoides. Can. J. Microbiol. 52: 130-139. doi:10.1139/w05-110. PMID:16541149.
31. Windhorst, S., Frank, E., Geogieva, D.N., Genov, N., Buck, F., Borowski, P., and Weber, W. 2002. The major extracellular protease of the nosocomial pathogen Stenotrophomonas maltophilia. J. Biol. Chem. 277: 11042-11049. doi:10.1074/jbc.M109525200. PMID:11796713.
32. Yang, J., Huang, X.W., Tian, B.Y., and Zhang, K.Q. 2005. Isotation and characterization of a serine protease from the nematophagous fungus Lecanicillium psalliotae, displaying nematicidal activity. Biotechnol. Lett. 27: 1123-1128. doi:10.1007/s10529-005-8461-0. PMID:16132863.
33. Yang, J., Li, J., Liang, L.M., Tian, B.Y., Zhang, Y., and Zhang, K.Q. 2007a. Cloning and characterization of a cuticle-degrading protease from the nematode-trapping fungus Arthrobotrys conoides. Arch. Microbiol. 188: 167-174. doi:10.1007/s00203-007-0233-x. PMID:17390124.
34. Yang, J., Liang, L.M., Zhang, Y., Li, J., Zhang, L., Ye, F.P., et al. 2007b. Purification and cloning of a novel serine protease from the nematode-trapping fungus Dactylellina varietas and its potential roles in infection against nematodes. Appl. Microbiol. Biotechnol. 75: 557-565. doi:10.1007/s00253-007-0839-6. PMID:17294185.
35. Yang, J., Tian, B.Y., Liang, L.M., and Zhang, K.Q. 2007c. Extracellular enzymes and the pathogenesis of nematophagous fungi. Appl. Microbiol. Biotechnol. 75: 21-31. doi:10.1007/s00253-007-0881-4. PMID:17318531.
36. Yoon, J.H., Kim, I.G., Kang, K.H., Oh, T.K., and Park, Y.H. 2003. Bacillus marisflavi sp. nov., and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea. Int. J. Syst. Evol. Microbiol. 53: 1297-1303. doi:10.1099/ijs.0.02365-0. PMID:13130010.
37. Zhao, M.L., Huang, J.S., Mo, M.H., and Zhang, K.Q. 2005. A potential virulence factor involved in fungal pathogenicity: serinelike protease activity of nematophagous fungus Clonostachys rosea. Fungal Divers. 19: 217-234.
38. Zhou, X., Kaya, H.K., Heungens, K., and Heidi, G.B. 2002. Response of ants to a deterrent factor(s) produced by the symbiotic bacteria of entomopathogenic nematodes. Appl. Environ. Microbiol. 68: 6202-6209. doi:10.1128/AEM.68.12.6202-6209.2002. PMID:12450845.