Expression, purification, and characterization of recombinant human keratinocyte growth factor-2 in Pichia pastoris

Journal of Biotechnology

Volumn 132, Issue 1, 2007, Pages 44-48

  Wang, Yanchun ^a   Yuan, Shengling ^a   Wang, Peng ^a   Liu, Xiangxin ^a   Zhan, Dewen ^a   Zhang, Zhaoshan ^a  

^a BEIJING INSTITUTE OF BIOTECHNOLOGY   (China)

Author keywords

Heterologous expression; KGF 2; Pichia pastoris

Indexed keywords

HETEROLOGOUS EXPRESSION; PICHIA PASTORIS; WESTERN BLOT ANALYSIS;

AFFINITY CHROMATOGRAPHY; BIOACTIVITY; FIBROBLASTS; METHANOL; PROTEINS;

CELL GROWTH;

FIBROBLAST GROWTH FACTOR 10; HEPARIN; METHANOL; PLASMID VECTOR; RECOMBINANT PROTEIN;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELL STRAIN 3T3; CLONING; CONTROLLED STUDY; CULTURE TECHNIQUE; EMBRYO; FUNGAL STRAIN; FUNGUS CULTURE; MOUSE; NONHUMAN; PICHIA PASTORIS; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SEQUENCE ANALYSIS; SHAKE FLASK CULTURE; WESTERN BLOTTING;

BASE SEQUENCE; BIOTECHNOLOGY; BLOTTING, WESTERN; DNA PRIMERS; FIBROBLAST GROWTH FACTOR 10; GENE EXPRESSION; GENETIC VECTORS; HUMANS; PICHIA; PLASMIDS; RECOMBINANT PROTEINS;

PICHIA PASTORIS;

EID: 34848916694     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2007.08.024     Document Type: Article

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