Optimization of chimeric HIV-1 virus-like particle production in a baculovirus-insect cell expression system

Biotechnology Progress

Volumn 25, Issue 4, 2009, Pages 1153-1160

  Pillay, Sirika ^a   Meyers, Ann ^a   Williamson, Anna Lise ^b,c   Rybicki, Edward P ^a  

^a UNIVERSITY OF CAPE TOWN   (South Africa)

^b UNIVERSITY OF CAPE TOWN   (South Africa)

^c UNIVERSITY OF CAPE TOWN   (South Africa)

Author keywords

Chimera; Gag; HIV; RT; Vaccine

Indexed keywords

BACULOVIRUS; CELL DENSITY; CHIMERA; EXPRESSION SYSTEM; FAVORABLE CONDITIONS; GAG; HIGHER YIELD; HIV; INFLUENCING FACTOR; INSECT CELL LINES; INSECT CELLS; MULTIPLICITY OF INFECTIONS; OPTIMAL CONDITIONS; PROCESS EFFICIENCY; RECOMBINANT PROTEIN; RT; TRICHOPLUSIA NI; VIRUS-LIKE PARTICLES; WESTERN BLOTS;

CELL CULTURE; OPTIMIZATION; PROTEINS; VACCINES;

CELL MEMBRANES;

GAG PROTEIN; HUMAN IMMUNODEFICIENCY VIRUS VACCINE; RECOMBINANT PROTEIN;

ANIMAL; ARMYWORM; BACULOVIRUS; CELL LINE; CONFERENCE PAPER; EVALUATION; GENE EXPRESSION; GENE VECTOR; GENETICS; HUMAN IMMUNODEFICIENCY VIRUS 1; METABOLISM; METHODOLOGY; PHYSIOLOGY; VIRUS CULTURE; VIRUS REPLICATION;

AIDS VACCINES; ANIMALS; BACULOVIRIDAE; CELL LINE; GAG GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; GENE EXPRESSION; GENETIC VECTORS; HIV-1; RECOMBINANT PROTEINS; SPODOPTERA; VIRUS CULTIVATION; VIRUS REPLICATION;

HEXAPODA; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; TRICHOPLUSIA NI;

EID: 69149091472     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1002/btpr.187     Document Type: Conference Paper

References (24)

1. UNAIDS, AIDS epidemic update 2007. Available at: http://data.unaids.org/ pub/EPISlides/2007/2007-epiupdate-en.pdf. 2007.
2. Yao Q, Bu Z, Vzorov A, Yang C, Compans RW. Virus-like particle and DNA-based candidate AIDS vaccines. Vaccine. 2003; 21: 638-643.
3. Noad R, Roy P. Virus-like particles as immunogens. Trends Microbiol. 2003; 11: 438-444.
4. Nabel GJ. HIV vaccine strategies. Vaccine. 2002; 20: 1945-1947.
5. Doan LX, Li M, Chen C, Yao Q. Virus-like particles as HIV-1 vaccines. Rev Med Virol. 2005; 15: 75-88.
6. Kost TA, Condreay JP, Jarvis DL. Baculovirus as versatile vectors for protein expression in insect and mammalian cells. Nat Biotechnol. 2005; 23: 567-575.
7. Hunt I. From gene to protein: a review of new and enabling technologies for multi-parallel protein expression. Protein Expr Purif. 2005; 40: 1-22.
8. Halsey RJ, Tanzer FL, Meyers A, Pillay S, Lynch A, Shephard E, Williamson AL, Rybicki EP. Chimaeric HIV-1 subtype C Gag molecules with large in-frame C-terminal polypeptide fusions form virus-like particles. Virus Res. 2008; 133: 259-268.
9. Deml L, Wild J, Wagner R. Virus-like particles: a novel tool for the induction and monitoring of both T-helper and cytotoxic T-lymphocyte activity. Methods Mol Med. 2004; 94: 133-157.
10. Guide to Baculovirus Expression Vector Systems (BEVS) and Insect cell Culture Techniques: Instruction manual. Invitrogen Life Technologies Inc., CA, USA, 2001.
11. Hink WF, Thomsen DR, Davidson DJ, Meyer AL, Castellino FJ. Expression of three recombinant proteins using baculovirus vectors in 23 insect cell lines. Biotechnol Prog. 1991; 7: 9-14.
12. Wickham TJ, Nemerow GR. Optimization of growth methods and recombinant protein production in BTI-Tn-5B1-4 insect cells using the baculovirus expression system. Biotechnol Prog. 1993; 9: 25-30.
13. Masemola A, Mashishi T, Khoury G, Mohube P, Mokgotho P, Vardas E, Colvin M, Zijenah L, Katzenstein D, Musonda R, Allen S, Kumwenda N, Taha T, Gray G, McIntyre J, Karim SA, Sheppard HW, Gray CM. Hierarchical targeting of subtype C human immunodeficiency virus type 1 proteins by CD8+ T cells: correlation with viral load. J Virol. 2004; 78: 3233-3243.
14. Luo L, Li Y, Cannon PM, Kim S, Kang CY. Chimeric gag-V3 virus-like particles of human immunodeficiency virus induce virus-neutralizing antibodies. Proc Natl Acad Sci USA. 1992; 89: 10527-10531.
15. Nakagawa T, Stadler BM, De Weck AL. Determination of rat mast cells by flow-cytometry. J Immunol Methods. 1980; 33: 87-92.
16. O'Reilly DR, Miller LK, Lucknow VA. Baculovirus Expression Vectors: A Laboratory Manual. New York: Oxford University Press, Inc.; 1994.
17. R Development Core Team R. A Language and Environment for Statistical Computing. Vienna, Austria: R Foundation for Statistical Computing; 2006.
18. Montgomery DC. Design and Analysis of Experiments, 6th e.d. Singapore: John Wiley & Sons; 2005.
19. Wagner R, Deml L, Schirmbeck R, Niedrig M, Reimann J, Wolf H. Construction, expression, and immunogenicity of chimeric HIV-1 virus-like particles. Virology. 1996; 220: 128-140.
20. Maruniak JE, Garcia-Canedo A, Rodrigues JJ. Cell lines used for the selection of recombinant baculovirus. In Vitro Cell Dev Biol Anim. 1994; 30A: 283-286.
21. Wickham TJ, Davis T, Granados RR, Shuler ML, Wood HA. Screening of insect cell lines for the production of recombinant proteins and infectious virus in the baculovirus expression system. Biotechnol Prog. 1992; 8: 391-396.
22. Hu YC, Bentley WE. Enhancing yield of infectious Bursal disease virus structural proteins in baculovirus expression systems: focus on media, protease inhibitors, and dissolved oxygen. Biotechnol Prog. 1999; 15: 1065-1071.
23. Hu YC. Baculovirus as a highly efficient expression vector in insect and mammalian cells. Acta Pharmacol Sin. 2005; 26: 405-416.
24. Modrow S, Kattenbeck B, von Poblotzki A, Niedrig M, Wagner R, Wolf H. The gag proteins of human immunodeficiency virus type 1: mechanisms of virus assembly and possibilities for interference. Med Microbiol Immunol. 1994; 183: 177-194.