Retrieval of trophoblast cells from the cervical canal for prediction of abnormal pregnancy: A pilot study

Human Reproduction

Volumn 24, Issue 9, 2009, Pages 2086-2092

  Imudia, Anthony N ^a   Suzuki, Yoko ^a   Kilburn, Brian A ^a   Yelian, Frank D ^a   Diamond, Michael P ^a   Romero, Roberto ^a,b   Armant, D Randall ^a,b,c  

^a WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^c WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Blighted ovum; Cervix; Ectopic pregnancy; First trimester; Trophoblast

Indexed keywords

CELL MARKER; HLA G ANTIGEN; MONOCLONAL ANTIBODY;

ACIDIFICATION; ADULT; ARTICLE; BLIGHTED OVUM; CELL COUNT; CONTROLLED STUDY; CYTOTROPHOBLAST; ECTOPIC PREGNANCY; FEMALE; FIRST TRIMESTER PREGNANCY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MAJOR CLINICAL STUDY; NULLIPARA; PILOT STUDY; PREGNANCY; RECEIVER OPERATING CHARACTERISTIC; SENSITIVITY AND SPECIFICITY; TROPHOBLAST; UTERINE CERVIX;

ADOLESCENT; ADULT; CERVIX UTERI; FEMALE; HISTOCOMPATIBILITY ANTIGENS CLASS I; HLA ANTIGENS; HUMANS; MIDDLE AGED; PREGNANCY; PREGNANCY COMPLICATIONS; PREGNANCY TRIMESTER, FIRST; PREGNANCY, ECTOPIC; TROPHOBLASTS;

EID: 68949211884     PISSN: 02681161     EISSN: 14602350     Source Type: Journal    
DOI: 10.1093/humrep/dep206     Document Type: Article

References (20)

1. Adinolfi M, Sherlock J, Tutschek B, Halder A, Delhanty J, Rodeck C. Detection of fetal cells in transcervical samples and prenatal diagnosis of chromosomal abnormalities. Prenat Diagn 1995;15:943-949.
2. Bulmer JN, Rodeck C, Adinolfi M. Immunohistochemical characterization of cells retrieved by transcervical sampling in early pregnancy. Prenat Diagn 1995;15:1143-1153.
3. Bulmer JN, Cioni R, Bussani C, Cirigliano V, Sole F, Costa C, Garcia P, Adinolfi M. HLA-G positive trophoblastic cells in transcervical samples and their isolation and analysis by laser microdissection and QF-PCR. Prenat Diagn 2003;23:34-39.
4. Bussani C, Cioni R, Scarselli B, Barciulli F, Bucciantini S, Simi P, Fogli A, Scarselli G. Strategies for the isolation and detection of fetal cells in transcervical samples. Prenat Diagn 2002;22:1098-1101.
5. Bussani C, Cioni R, Mattei A, Fambrini M, Marchionni M, Scarselli G. Prenatal diagnosis of common aneuploidies in transcervical samples using quantitative fluorescent-PCR analysis. Mol Diagn Ther 2007;11:117-121.
6. Chumbley G, King A, Gardner L, Howlett S, Holmes N, Loke YW. Generation of an antibody to HLA-G in transgenic mice and demonstration of the tissue reactivity of this antibody. J Reprod Immunol 1994;27:173-186.
7. Cioni R, Bussani C, Scarselli B, Bucciantini S, Barciulli F, Scarselli G. Fetal cells in cervical mucus in the first trimester of pregnancy. Prenat Diagn 2003;23:168-171.
8. Cioni R, Bussani C, Scarselli B, Bucciantini S, Marchionni M, Scarselli G. Comparison of two techniques for transcervical cell sampling performed in the same study population. Prenat Diagn 2005;25:198-202.
9. ErgIn T, Baltaci V, Zeyneloglu HB, Duran EH, Ergenel IM, Batioglu S. Non-invasive early prenatal diagnosis using fluorescent in situ hybridization on transcervical cells: comparison of two different methods for retrieval. Eur J Obstet Gynecol Reprod Biol 2001;95: 37-41.
10. Fang CN, Kan YY, Hsiao CC. Detection of fetal cells from transcervical mucus plug before first-trimester termination of pregnancy by cytokeratin-7 immunohistochemistry. J Obstet Gynaecol Res 2005; 31:500-507.
11. Fejgin MD, Diukman R, Cotton Y, Weinstein G, Amiel A. Fetal cells in the uterine cervix: a source for early non-invasive prenatal diagnosis. Prenat Diagn 2001;21:619-621.
12. Katz-Jaffe MG, Mantzaris D, Cram DS. DNA identification of fetal cells isolated from cervical mucus: potential for early non-invasive prenatal diagnosis. BJOG 2005;112:595-600.
13. Loke YW, King A, Burrows T, Gardner L, Bowen M, Hiby S, Howlett S, Holmes N, Jacobs D. Evaluation of trophoblast HLA-G antigen with a specific monoclonal antibody. Tissue Antigens 1997;50:135-146.
14. Massari A, Novelli G, Colosimo A, Sangiuolo F, Palka G, Calabrese G, Camurri L, Ghirardini G, Milani G, Giorlandino C et al. Non-invasive early prenatal molecular diagnosis using retrieved transcervical trophoblast cells. Hum Genet 1996;97:150-155.
15. Miller D, Briggs J, Rahman MS, Griffith-Jones M, Rane V, Everett M, Lilford RJ, Bulmer JN. Transcervical recovery of fetal cells from the lower uterine pole: reliability of recovery and histological/ immunocytochemical analysis of recovered cell populations. Hum Reprod 1999;14:521- 531.
16. Rodeck C, Tutschek B, Sherlock J, Kingdom J. Methods for the transcervical collection of fetal cells during the first trimester of pregnancy. Prenat Diagn 1995;15:933-942.
17. Sherlock J, Halder A, Tutschek B, Delhanty J, Rodeck C, Adinolfi M. Prenatal detection of fetal aneuploidies using transcervical cell samples. J Med Genet 1997;34:302-305.
18. Shiverick KT, King A, Frank H, Whitley GS, Cartwright JE, Schneider H. Cell culture models of human trophoblast II: trophoblast cell lines - a workshop report. Placenta 2001;22:S104-S106.
19. Tutschek B, Sherlock J, Halder A, Delhanty J, Rodeck C, Adinolfi M. Isolation of fetal cells from transcervical samples by micromanipulation: molecular confirmation of their fetal origin and diagnosis of fetal aneuploidy. Prenat Diagn 1995;15:951-960.
20. Ventura SJ, Abma JC, Mosher WD, Henshaw SK. Estimated pregnancy rates by outcome for the United States, 1990-2004. Natl Vital Stat Rep 2008;56:1-2528.