Detection of fetal cells from transcervical mucus plug before first-trimester termination of pregnancy by cytokeratin-7 immunohistochemistry

Journal of Obstetrics and Gynaecology Research

Volumn 31, Issue 6, 2005, Pages 500-507

  Fang, Chun Neng ^a,c   Kan, Yuan Yee ^a,b   Hsiao, Chang Chun ^c  

^a KAOHSIUNG VETERANS GENERAL HOSPITAL   (Taiwan)

^b NATIONAL YANG MING UNIVERSITY   (Taiwan)

^c CHANG GUNG UNIVERSITY   (Taiwan)

Author keywords

Cytokeratin 7; Fetal cells; Immunohistochemistry; Pregnancy; Transcervical mucus

Indexed keywords

CYTOKERATIN 7; MONOCLONAL ANTIBODY;

AMNION; CELL STRUCTURE; CHORION; CHORION VILLUS; CHROMOSOME DISORDER; CONFERENCE PAPER; CONTROLLED STUDY; FEMALE; FETUS CELL; FIRST TRIMESTER PREGNANCY; GENETIC DISORDER; GESTATIONAL AGE; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; MAJOR CLINICAL STUDY; MICROSCOPY; PREGNANCY; PREGNANCY TERMINATION; PRENATAL DIAGNOSIS; SAMPLING; TISSUE FIXATION; UTERINE CERVIX MUCUS; UTERUS CAVITY;

ABORTION, INDUCED; ANTIBODIES, MONOCLONAL; CERVIX MUCUS; FEMALE; FETUS; HUMANS; IMMUNOHISTOCHEMISTRY; KERATIN-7; KERATINS; PREGNANCY; PREGNANCY TRIMESTER, FIRST; PRENATAL DIAGNOSIS; SUCTION; TROPHOBLASTS;

EID: 30344433247     PISSN: 13418076     EISSN: 14470756     Source Type: Journal    
DOI: 10.1111/j.1447-0756.2005.00329.x     Document Type: Conference Paper

References (16)

1. Falcinelli C, Battafarano S, Neri C et al. First-trimester fetal sex prediction by deoxyribonucleic acid analysis of maternal peripheral blood. Am J Obstet Gynecol 1999; 181: 675-680.
2. Fukushima A, Utsugisawa Y, Wada Y, Mizusawa N, Horiuchi S, Kagabu T. The application of magnetic cell sorter (MACS) to detect fetal cells in maternal peripheral blood. J Obstet Gynaecol Res 2001; 27: 155-162.
3. Kingdom J, Sherlock J, Rodeck C, Adinolfi M. Detection of trophoblast cells in transcervical samples collected by lavage or cytobrush. Obstet Gynecol 1995; 86: 283-288.
4. Adinolfi M, Sherlock J. Fetal cells in transcervical samples at an early stage of gestation. J Hum Genet 2001; 46: 99-104.
5. Kawashima M, Kitagawa M. An immunohistochemical study of cadherin 5 (VE-cadherin) in vascular endothelial cells in placentas with gestosis. J Obstet Gynaecol Res 1998; 24: 375-384.
6. Blaschitz A, Weiss U, Dohr G, Desoye G. Antibody reaction patterns in first trimester placenta: Implications for trophoblast isolation and purity screening. Placenta 2000; 21: 733-741.
7. Daryani YP, Barker GH, Penna LK, Patton MA. Transcervical sampling as a means of detection of fetal cells during the first trimester of pregnancy. Am J Obstet Gynecol 2000; 183: 752-754.
8. Bulmer JN, Rodeck C, Adinolfi M. Immunohistochemical characterization of cells retrieved by transcervical sampling in early pregnancy. Prenat Diagn 1995; 15: 1143-1153.
9. Bussani C, Cioni R, Scarselli B et al. Strategies for the isolation and detection of fetal cells in transcervical samples. Prenat Diagn 2002; 22: 1098-1101.
10. Haigh T, Chen C, Jones CJ, Aplin JD. Studies of mesenchymal cells from 1st trimester human placenta: expression of cytokeratin outside the trophoblast lineage. Placenta 1999; 20: 615-625.
11. Miller D, Briggs J, Rahman MS et al. Transcervical recovery of fetal cells from the lower uterine pole: Reliability of recovery and histological/immunocytochemical analysis of recovered cell populations. Hum Reprod 1999; 14: 521-531.
12. Bulmer JN, Cioni R, Bussani C et al. HLA-G positive trophoblastic cells in transcervical samples and their isolation and analysis by laser microdissection and QF-PCR. Prenat Diagn 2003; 23: 34-39.
13. Adinolfi M, Davies A, Sharif S, Soothill P, Rodeck C. Detection of trisomy 18 and Y-derived sequences in fetal nucleated cells obtained by transcervical flushing. Lancet 1993; 342: 403-404.
14. Adinolfi M, Pertl B, Sherlock J. Rapid detection of aneuploidies by microsatellite and the quantitative fluorescent polymerase chain reaction. Prenat Diagn 1997; 17: 1299-1311.
15. Adinolfi M, Sherlock J. First trimester prenatal diagnosis using transcervical cells: An evaluation. Hum Reprod Update 1997; 3: 383-392.
16. Chang SD, Lin SL, Chu KK, Hsi BL. Minimally-invasive early prenatal diagnosis using fluorescence in situ hybridization on samples from uterine lavage. Prenat Diagn 1997; 17: 1019-1025.