A single amino acid substitution in PmrB is associated with polymyxin B resistance in clinical isolate of Pseudomonas aeruginosa

FEMS Microbiology Letters

Volumn 298, Issue 2, 2009, Pages 249-254

  Abraham, Neethu ^a   Kwon, Dong H ^a,b  

^a LONG ISLAND UNIVERSITY   (United States)

^b BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

pmrAB (two component regulatory system); Polymyxin B resistance; Pseudomonas aeruginosa

Indexed keywords

AMINO ACID; AMINO ACID PMRB; CYSTEINE; METHIONINE; POLYMYXIN B; THREONINE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ANIMAL EXPERIMENT; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERICIDAL ACTIVITY; BACTERIUM ISOLATION; CLONE; CLONING VECTOR; DNA SEQUENCE; MINIMUM INHIBITORY CONCENTRATION; MOLECULAR BIOLOGY; NONHUMAN; NUCLEOTIDE SEQUENCE; NULL ALLELE; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; REGULATOR GENE; SINGLE NUCLEOTIDE POLYMORPHISM;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; COLONY COUNT, MICROBIAL; DNA, BACTERIAL; DRUG RESISTANCE, BACTERIAL; GENETIC COMPLEMENTATION TEST; HUMANS; MICROBIAL VIABILITY; MOLECULAR SEQUENCE DATA; MUTATION, MISSENSE; POLYMYXIN B; PSEUDOMONAS AERUGINOSA; PSEUDOMONAS INFECTIONS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; TRANSCRIPTION FACTORS;

PSEUDOMONAS AERUGINOSA;

EID: 68949210558     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2009.01720.x     Document Type: Article

References (21)

1. Arnold TM, Forrest GN Messmer KJ (2007) Polymyxin antibiotics for gram-negative infections. Am J Health-Syst Ph 64 : 819 826.
2. Clinical and Laboratory Standards Institute (CLIS) (2007) Performance Standards for Antimicrobial Susceptibility Testing: Seventeenth Informational Supplement M100-S17. CLIS, Wayne, PA.
3. Falagas ME Bliziotis IA (2007) Pandrug-resistant Gram-negative bacteria: the dawn of the post-antibiotic era? Int J Antimicrob Ag 29 : 630 636.
4. Falagas ME, Rafailidis PI, Matthaiou DK, Virtzili S, Nikita D Michalopoulos A (2008) Pandrug-resistant Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii infections: characteristics and outcome in a series of 28 patients. Int J Antimicrob Ag 32 : 450 454.
5. Gambello MJ Iglewski BH (1991) Cloning and characterization of the Pseudomonas aeruginosa lasR gene, a transcriptional activator of elastase expression. J Bacteriol 173 : 3000 3009.
6. Hancock RE Speert DP (2000) Antibiotic resistance in Pseudomonas aeruginosa: mechanisms and impact on treatment. Drug Resist Update 3 : 247 255.
7. Kwon DH Lu CD (2007) Polyamine effects on antibiotic susceptibility in bacteria. Antimicrob Agents Ch 51 : 2070 2077.
8. Macfarlane EL, Kwasnicka A, Ochs MM Hancock RE (1999) PhoP-PhoQ homologues in Pseudomonas aeruginosa regulate expression of the outer-membrane protein OprH and polymyxin B resistance. Mol Microbiol 34 : 305 316.
9. Macfarlane EL, Kwasnicka A Hancock RE (2000) Role of Pseudomonas aeruginosa PhoP-phoQ in resistance to antimicrobial cationic peptides and aminoglycosides. Microbiology 146 : 2543 2554.
10. Mahenthiralingam E, Campbell ME, Foster J, Lam JS Speert DP (1996) Random amplified polymorphic DNA typing of Pseudomonas aeruginosa isolates recovered from patients with cystic fibrosis. J Clin Microbiol 34 : 1129 1135.
11. McPhee JB, Lewenza S Hancock RE (2003) Cationic antimicrobial peptides activate a two-component regulatory system, PmrA-PmrB, that regulates resistance to polymyxin B and cationic antimicrobial peptides in Pseudomonas aeruginosa. Mol Microbiol 50 : 205 217.
12. McPhee JB, Bains M, Winsor G et al 2006) Contribution of the PhoP-PhoQ and PmrA-PmrB two-component regulatory systems to Mg2+-induced gene regulation in Pseudomonas aeruginosa. J Bacteriol 188 : 3995 4006.
13. Moskowitz SM, Ernst RK Miller SI (2004) PmrAB, a two-component regulatory system of Pseudomonas aeruginosa that modulates resistance to cationic antimicrobial peptides and addition of aminoarabinose to lipid A. J Bacteriol 186 : 575 579.
14. Mulcahy H, Charron-Mazenod L Lewenza S (2008) Extracellular DNA chelates cations and induces antibiotic resistance in Pseudomonas aeruginosa biofilms. PLoS Pathog 4 : e1000213.
15. Oliver A, Canton R, Campo P, Baquero F Blazquez J (2000) High frequency of hypermutable Pseudomonas aeruginosa in cystic fibrosis lung infection. Science 288 : 1251 1254.
16. Park SM, Lu CD Abdelal AT (1997) Cloning and characterization of argR, a gene that participates in regulation of arginine biosynthesis and catabolism in Pseudomonas aeruginosa PAO1. J Bacteriol 179 : 5300 5308.
17. Rowe SM, Miller S Sorscher EJ (2005) Cystic fibrosis. New Engl J Med 352 : 1992 2001.
18. Schweizer HP (1991) Escherichia-Pseudomonas shuttle vectors derived from pUC18/19. Gene 97 : 109 121.
19. Stibitz S, Black W Falkow S (1986) The construction of a cloning vector designed for gene replacement in Bordetella pertussis. Gene 50 : 133 140.
20. Wang CY, Jerng JS, Chen KY, Lee LN, Yu CJ, Hsueh PR Yang PC (2006) Pandrug-resistant Pseudomonas aeruginosa among hospitalised patients: clinical features, risk-factors and outcomes. Clin Microbiol Infect 12 : 63 68.
21. Yeaman MR Yount NY (2003) Mechanisms of antimicrobial peptide action and resistance. Pharmacol Rev 55 : 27 55.