A quantitative resazurin assay to determinate the viability of Trichomonas vaginalis and the cytotoxicity of organic solvents and surfactant agents

Experimental Parasitology

Volumn 123, Issue 2, 2009, Pages 195-198

  Duarte, Mariana ^a   Giordani, Raquel Brandt ^a   De Carli, Geraldo Attilio ^b   Zuanazzi, José Angelo ^a   Macedo, Alexandre José ^a   Tasca, Tiana ^a  

^a FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

^b PONTIFICAL CATHOLIC UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

Author keywords

Fluorimetric assay; Organic solvents; Resazurin; Surfactant agents; Trichomonas vaginalis; Vehicles

Indexed keywords

2 PROPANOL; ACETONE; ALCOHOL; DIMETHYL SULFOXIDE; METHANOL; METRONIDAZOLE; ORGANIC SOLVENT; POLYSORBATE 20; POLYSORBATE 80; RESAZURIN; SURFACTANT; TRITON X 100;

ANTIPROTOZOAL ACTIVITY; ARTICLE; CONTROLLED STUDY; CYTOTOXICITY; DRUG SENSITIVITY; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PARASITE ISOLATION; PARASITE VIABILITY; PRIORITY JOURNAL; QUANTITATIVE ASSAY; TRICHOMONAS VAGINALIS; TROPHOZOITE;

2-PROPANOL; ACETONE; ANIMALS; DIMETHYL SULFOXIDE; ETHANOL; FLUOROMETRY; INDICATORS AND REAGENTS; METHANOL; OCTOXYNOL; OXAZINES; PARASITIC SENSITIVITY TESTS; POLYSORBATES; SOLVENTS; SURFACE-ACTIVE AGENTS; TRICHOMONAS VAGINALIS; XANTHENES;

TRICHOMONAS; TRICHOMONAS VAGINALIS;

EID: 68949167646     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2009.07.002     Document Type: Article

References (25)

1. Aldrete M.E.C., Salgado-Zamora H., Luna J., Meléndez E., and Meráz-Ríos M.A. A high throughput colorimetric and fluorimetric microassay for the evaluation of nitroimidazole derivatives anti-Trichomonas activity. Toxicology In Vitro 19 (2005) 1045-1050
2. Carrillo-Muñoz A.J., Quindos G., Ruesga M., del Valle O., Pemán J., Cantón E., Hernández-Molina J.M., and Santos P. In vitro antifungal susceptibility testing of filamentous fungi with sensititre yeast one. Mycoses 49 (2006) 293-297
3. Cherpes T., Wiesenfeld H., Melan M., Kant J.A., Consentino L.A., Meyn L.A., and Hillier S.L. The associations between pelvic inflammatory disease, Trichomonas vaginalis infection, and positive herpes simplex virus type 2 serology. Sexually Transmitted Diseases 33 (2006) 747-752
4. Cotch M.F., Pastorek J.G., Nugent R.P., Hillier S.L., Gibbs R.S., Martin D.H., Eschenbach D.A., Edelman R., Carey J.C., Regan J.A., Krohn M.A., Klebanoff M.A., Rao A.V., and Rhoads G.G. Trichomonas vaginalis associated with low birth weight and preterm delivery. The vaginal infections and prematurity study group. Sexually Transmitted Diseases 24 (1997) 353-360
5. 3H-thymidine incorporation assay. Journal of Clinical Laboratory Analysis 9 (1995) 89-95
6. Diamond L.S. The establishment of various Trichomonas of animals and man in axenic cultures. The Journal of Parasitology 43 (1957) 488-490
7. Fields R.D., and Lancaster M.V. Dual-attribute continuous monitoring of cell proliferation/cytotoxicity. American Biotechnology Laboratory 11 (1993) 48-50
8. Giordani, R.B., De Almeida, M.V., Fernandes, E., França da Costa, C., De Carli, G.A., Tasca, T., Zuanazzi, J.A., 2008. Anti-Trichomonas activity of synthetic lipophilic diamine and amino alcohol derivatives. Biomedicine & Pharmacotherapy. doi:10.1016/j.biopha.2008.10.002.
9. Goldstein F., Goldman M.B., and Cramer D.W. Relation of tubal infertility to a story of sexually transmitted diseases. American Journal of Epidemiology 137 (1993) 577-584
10. Helms D.J., Mosure D.J., Secor W.E., and Workowski K.A. Management of Trichomonas vaginalis in women with suspected metronidazole hypersensitivity. American Journal of Obstetrics and Gynecology 198 (2008) 370-377
11. Michel R.V., Borges F.P., Wiltuschnig R.C.M., Neves F.G., Ribeiro J., Vieiro R.C., Vieira P.B., Bohns G.R., Tasca T., and De Carli G.A. Prevalência da tricomonose em mulheres residentes na Vila dos Papeleiros em Porto Alegre, RS. Revista Brasileira de Análises Clínicas 38 (2006) 127-130
12. Moo-Puc R., Robledo D., and Freile-Pelegrin Y. Evaluation of selected tropical seaweeds for in vitro anti-trichomonal activity. Journal of Ethnopharmacology 120 (2008) 92-97
13. Müller M., Lossick J.G., and Gorell T.E. In vitro susceptibility of Trichomonas vaginalis to metronidazole and treatment outcome in vaginal trichomoniasis. Sexually Transmitted Diseases 15 (1988) 17-24
14. O'Brien J., Wilson I., Orton T., and Pognan F. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. European Journal of Biochemistry 267 (2000) 5421-5426
15. Palomino J.C., Martin A., Camacho M., Guerra H., Swings J., and Portaels F. Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy 46 (2002) 2720-2722
16. Peetters E., Nelis H.J., and Coenye T. Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates. Journal of Microbiological Methods 72 (2008) 157-165
17. Pettit R.K., Weber C.A., Kean M.J., Hoffmann H., Pettit G.R., Tan R., Franks K.S., and Horton M.L. Microplate alamar blue assay for Staphylococcus epidermidis biofilm susceptibility testing. Antimicrobial Agents and Chemotherapy 49 (2005) 2612-2617
18. Porcal G.V., Previtali C.M., and Bertolotti S.G. Photophysics of the phenoxazine dyes resazurin and resorufin in direct and reverse micelles. Dyes and Pigments 80 (2009) 206-211
19. Raz B., Iten M., Grether Buhlery Y., Kaminsky R., and Brun R. The Alamar Blue assay to determine drug sensitivity of African trypanosomes (T.b. rhodesiense and T.b. gambiense) in vitro. Acta Tropica 68 (1997) 139-147
20. Rojas L., Sariego I., Fraga J., Sarría C.A., Sarracent J., and Sanchez L. Use of in vitro cytoadherence assays in the comparative study of the virulence of isolates of Trichomonas vaginalis. Parasitology Research 93 (2004) 332-337
21. Upcroft J.A., Dunn L.A., Wright J.M., Benakli K., Upcroft P., and Vanelle P. 5-Nitroimidazole drugs effective against metronidazole-resistant Trichomonas vaginalis and Giardia duodenalis. Antimicrobial Agents and Chemotherapy 50 (2006) 344-347
22. Van Der Pol B., Kwok C., Pierre-Louis B., Rinaldi A., Salata R.A., Chen P.L., van de Wijgert J., Mmiro F., Mugerwa R., Chipato T., and Morrison C.S. Trichomonas vaginalis infection and human immunodeficiency virus acquisition in African women. The Journal of Infectious Diseases 197 (2008) 548-554
23. Viikki M., Pukkala E., Nieminen P., and Hakama M. Gynaecological infections as risk determinants of subsequent cervical neoplasia. Acta Oncologica 39 (2000) 71-75
24. Wei G.X., Campagna A.N., and Bobek L.A. Effect of MUC7 peptides on the growth of bacteria and on Streptococcus mutans biofilm. The Journal of Antimicrobial Chemotherapy 57 (2006) 1100-1109
25. World Health Organization, 2001. Global Prevalence and Incidence of Selected Curable Sexually Transmitted Infections. Overview and Estimates, WHO, Geneva, Switzerland.