Simple quantification of bacterial envelope-associated extracellular materials

Journal of Microbiological Methods

Volumn 78, Issue 3, 2009, Pages 302-306

  Ionescu, Michael ^a   Belkin, Shimshon ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

Centrifuge; Colanic acid; Exopolysaccharide; Extracellular matrix

Indexed keywords

ESCHERICHIA COLI PROTEIN; EXOPOLYSACCHARIDE; PROTEIN YJBG; SIGMA FACTOR RPOS; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL MEMBRANE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM MUTANT; BIOMASS; CELL MUTANT; CELL SURFACE; CENTRIFUGATION; CONTROLLED STUDY; ESCHERICHIA COLI K 12; EXTRACELLULAR FLUID; NONHUMAN; OPTICAL DENSITY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN SYNTHESIS; QUANTITATIVE ANALYSIS; WILD TYPE;

BACTERIAL PROTEINS; CELL WALL; CENTRIFUGATION; ESCHERICHIA COLI K12; GENE DELETION; POLYSACCHARIDES, BACTERIAL; SIGMA FACTOR;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 68949163030     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2009.06.020     Document Type: Article

References (17)

1. Brady S.L., Ng S.Y.M., and Jerrell F. Prokaryotic motility structures. Microbiology 149 (2003) 295-304
2. Datsenko K.A., and Wanner B.L. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. USA 97 (2000) 6640-6645
3. Duguid J.P. The demonstration of bacteria capsules and slime. J. Pathol. Bacteriol. 63 (1951) 673-685
4. Francez-Charlot A., Laugel B., Van Gemert A., Dubarry N., Wiorowski F., Castanié-Cornet M.P., Gutierrez C., and Cam K. RcsCDB His-Asp phosphorelay system negatively regulates the flhDC operon in Escherichia coli. Mol. Microbiol. 49 (2003) 823-832
5. Gebbink M.F., Claessen D., Bouma B., Dijkhuizen L., and Wösten H.A. Amyloids - a functional coat for microorganisms. Nat. Rev., Microbiol. 3 (2005) 333-341
6. Gottesman S., Trisler P., and Torres-Cabassa A. Regulation of capsular polysaccharide synthesis in Escherichia coli K-12: characterization of three regulatory genes. J. Bacteriol. 162 (1985) 1111-1119
7. Ionescu M., and Belkin S. Overproduction of exopolysaccharides by an Escherichia coli K12 rpoS mutant in response to osmotic stress. Appl. Environ. Microbiol. 75 (2009) 483-492
8. Levine M.M., Bergquist E.J., Nalin D.R., Waterman D.H., Hornick R.B., Young C.R., and Sotman S. Escherichia coli strains that cause diarrhea but do not produce heat-labile or heat-stable enterotoxins and are non-invasive. Lancet 1 (1978) 1119-1112
9. Loewen P.C., and Triggs B.L. Genetic mapping of katF, a locus that with katE affects the synthesis of a second catalase species in Escherichia coli. J. Bacteriol. 160 (1984) 668-675
10. Majdalani N., and Gottesman S. The Rcs phosphorelay: a complex signal transcription system. Annu. Rev. Microbiol. 59 (2005) 379-405
11. Peleg A., Shifrin Y., Ilan O., Nadler-Yona C., Nov S., Koby S., Baruch K., Altuvia S., Elgrably-Weiss M., Abe C.M., Knutton S., Saper M.A., and Rosenshine I. Identification of an Escherichia coli operon required for formation of O-antigen capsule. J. Bacteriol. 187 (2005) 5259-5266
12. Pertoft H., and Laurent T.C. In: Catsimpoolas N. (Ed). Methods of Cell Separation Vol. I: Isopycnic Separation of Cells and Cell Organelles by Centrifugation in Modified Colloid Silica Gradients (1977), Plenum, New York, USA 25-65
13. Pizarro-Cerda J., and Cossart P. Bacterial adhesion and entry into host cells. Cell 124 (2006) 715-727
14. Silhavy T.J., Berman M.L., and Enquist L.W. Experiments with Gene Fusions (1984), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA
15. Whitfield C. Biosynthesis and assembly of capsular polysaccharides in Escherichia coli. Annu. Rev. Biochem. 75 (2006) 39-68
16. Yu D., Ellis H.M., Lee E.C., Jenkins N.A., Copeland N.G., and Court D.L. An efficient recombination system for chromosome engineering in Escherichia coli. Proc. Natl. Acad. Sci. USA 97 (2000) 5978-5983
17. Yuval B., Kaspi R., Shloush S., and Warburg M.S. Nutritional reserves regulate male participation in Mediterranean fruit fly leks. Ecol. Entomol. 23 (1998) 211-215