Separation and determination of asiaticoside, asiaticoside-B and madecassoside in centella asiatica total triterpenoid saponins by HPLC

Journal of Liquid Chromatography and Related Technologies

Volumn 32, Issue 13, 2009, Pages 1891-1900

  Xing, Huabin ^a   Su, Baogen ^a   Wang, Yangyang ^a   Yang, Yiwen ^a   Ren, Qilong ^a   Xiao, Wenguang ^a   Lu, Xiuyang ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Asiaticoside; Centella asiatica; HPLC; Isomer; Madecassoside; Separation

Indexed keywords

ASIATICOSIDE; ATLANTIS; CENTELLA ASIATICA; HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHODS; HIGH RESOLUTION; HPLC; MADECASSOSIDE; METHYL TERT BUTYL ETHER; MOBILE PHASE; MOBILE-PHASE COMPOSITION; REVERSED PHASE CHROMATOGRAPHY; STATIONARY PHASE; TRITERPENOID SAPONINS;

ACETONITRILE; CHROMATOGRAPHIC ANALYSIS; COLUMN CHROMATOGRAPHY; ETHERS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PHASE SEPARATION;

ISOMERS;

ASIATICOSIDE; ASIATICOSIDE B; PLANT GLYCOSIDE; SAPONIN; TRITERPENOID; UNCLASSIFIED DRUG;

ACCURACY; ARTICLE; CENTELLA ASIATICA; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOMER; REVERSED PHASE LIQUID CHROMATOGRAPHY; SEPARATION TECHNIQUE; VALIDATION PROCESS;

CENTELLA ASIATICA;

EID: 68949086223     PISSN: 10826076     EISSN: 1520572X     Source Type: Journal    
DOI: 10.1080/10826070903091597     Document Type: Article

References (22)

1. Singh, B.; Rastogi, R.P. A reinvestigation of the triterpenes of Centella asiatica III. Phytochemistry 1969, 8, 917-921.
2. Sahu, N.P.; Roy, S.K.; Mahato, S.B. Spectroscopic determination of structures of triterpenoid trisaccharides from Centella asiatica. Phytochemistry 1989, 28, 2852-2854.
3. Matsuda, H.; Morikawa, T.; Ueda, H.; Yoshikawa, M. Medicinal foodstuffs. XXVII. Saponin constituents of gotu kola (2): Structures of new ursane- and oleanane-type triterpene oligoglycosides, centellasaponins B, C, and D, from Centella asiatica cultivated in Sri Lanka. Chem. Pharm. Bull. 2001, 49 (10), 1368-1371.
4. Gnanapragasam, A.; Yogeeta, S.; Subhashini, R.; Ebenezar, K.K.; Sathish, V.; Devaki, T. Adriamycin induced myocardial failure in rats: Protective role of Centella asiatica. 2007, 294 (1-2), 55-63.
5. Gnanapragasam, A.; Ebenezar, K.K.; Sathish, V.; Govindaraju, P.; Devaki, T. Protective effect of Centella asiatica on antioxidant tissue defense system against adriamycin induced cardiomyopathy in rats. Life Sci. 2004, 76 (5), 585-597.
6. Sampson, J.H.; Raman, A.; Karlsen, G.; Navsaria, H.; Leigh, I.M. In vitro keratinocyte antiproliferant effect of Centella asiatica extract and triterpenoid saponins. Phytomedicine 2001, 8 (3), 230-235.
7. Shukla, A.; Rasik, A.M.; Jain, G.K.; Shankar, R.; Kulshrestha, D.K.; Dhawan, B.N. In vitro and in vivo wound healing activity of asiaticoside isolated from Centella asiatica. J. Ethnopharmacol. 1999, 65 (1), 1-11.
8. Cheng, C.L.; Koo, M.W.L. Effects of Centella asiatica on ethanol induced gastric mucosal lesions in rats. Life Sci. 2000, 67 (21), 2647-2653.
9. Bouillon, A.L.; Paris, G.S. Method for preparing a centelia asiatica extract rich in madecassoside and in terminoloside. US patent 2006=0106206A1 2006.
10. Oleszek, W.; Bialy, Z. Chromatographic determination of plant saponins - An update (2002-2005). J. Chromatogr. A. 2006, 1112, 78-91.
11. Ha, Y.W.; Nab, Y.C.; Seo, J.J.; Kima, S.N.; Linhardt, R.J.; Kim, Y.S. Qualitative and quantitative determination of ten major saponins in Platy-codi Radix by high performance liquid chromatography with evaporative light scattering detection and mass spectrometry. J. Chromatogr. A 2006, 1135, 27-35.
12. Rupasinghe, H.P.V.; Jackson, C.J.; Poysa, V.; Berardo, C.D.; Bewley, J.D.; Jenkinson, J. Soyasapogenol A and B distribution in soybean (Glycine max L. Merr.) in relation to seed physiology, genetic variability, and growing location. J. Agric. Food Chem. 2003, 51 (20), 5888-5894.
13. Müller, A.; Ganzera, M.; Stuppner, H. Analysis of phenolic glycosides and saponins in Primula elatior and Primula veris (primula root) by liquid chromatography, evaporative light scattering detection and mass spectrometry. J. Chromatogr. A 2006, 1112, 218-223.
14. Schaneberg, B.T.; Mikell, J.R.; Bedir, E.; Khan, I.A. An improve HPLC method for quantitative determination of six triterpenes in Centella asiatica extracts and commercial products. Pharmazie 2003, 58, 381-384.
15. Pan, J.; Kai, G.Q.; Yuan, C.X.; Jin. R.S. Separation and determination of the structural isomers of madecassoside by HPLC using β-cyclodextrin as mobile phase additive. Chromatographia 2007, 66 (1-2), 121-123.
16. Jin, M.C.; Yang, Y.W.; Su, B.G.; Ren, Q.L. Determination of soyasaponins Ba and Bb in human serum by high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry. J. Chroma-togr. B 2007, 846, 169-175.
17. Jin, M.C.; Yang, Y.W.; Su, B.G.; Ren, Q.L. Rapid quantification and characterization of soyasaponins by high-performance liquid chromatography coupled with electrospray mass spectrometry. J. Chromatogr. A 2006, 1108, 31-37.
18. Inamdar, P.K.; Yeole, R.D.; Ghogare, A.B. Determination of biologically active constituents in Centella asiatica. J. Chromatogr. A 1996, 742, 127-130.
19. Verma, R.K.; Bhartariya, K.G.; Gupta, M.M.; Kumar, S. Reverse-phase high performance liquid chromatography of asiaticoside in Centella asiatica. Phytochem. Anal. 1999, 10, 191-193.
20. Jia, G.T.; Lu, X.Y. Enrichment and purification of madecassoside and asiaticoside from Centella asiatica extracts with macroporous resins. J. Chromatogr. A 2008, 1993, 136-141.
21. Aziz, Z.A.; Davey, M.R.; Power, J.B.; Anthony, P.; Smith, R.M.; Lowe, K.C. Production of asiaticoside and madecassoside in Centella asiatica in vitro and in vivo. Biologia Plantarum 2007, 51 (1), 34-42.
22. t'Kindtl, R.; Alaerts, G.; Heyden, Y.V.; Deforce, D.; Bocxlaer, J.V. Broad-spectrum separations in metabolomics using enhanced polar LC stationary phases: a dedicated evaluation using plant metabolites. J. Sepn. Sci. 2007, 30, 2002-2011.