A critical appraisal of factors affecting the accuracy of results obtained when using flow cytometry in stem cell investigations: Where do you put your gates?

Cytometry Part A

Volumn 75, Issue 9, 2009, Pages 803-810

  Hughes, Owen R ^a,b   Stewart, Rebecca ^a   Dimmick, Ian ^a   Jones, Elizabeth A ^a,c  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

^c UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Doublet discriminator; Enzymatic dissociation; Gating strategies; Isotype controls; Stem cells

Indexed keywords

CD15 ANTIGEN; CHEMOKINE RECEPTOR CXCR4; STEM CELL FACTOR;

ACCURACY; ADULT; ANIMAL CELL; ANTIGEN EXPRESSION; ARTICLE; BONE MARROW CELL; CELL SELECTION; CELL SUBPOPULATION; CONTROLLED STUDY; DISSOCIATION; EMBRYONIC STEM CELL; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LYMPHOCYTE; MOUSE; NONHUMAN; PRIORITY JOURNAL; STEM CELL;

ANIMALS; BONE MARROW CELLS; CELL COUNT; CELL SEPARATION; CELL SURVIVAL; CELLS, CULTURED; EMBRYONIC STEM CELLS; FLOW CYTOMETRY; FUCOSYLTRANSFERASES; HUMANS; LYMPHOCYTES; MICE; MICE, INBRED C57BL; PROTO-ONCOGENE PROTEINS C-KIT; RECEPTORS, CXCR4; REPRODUCIBILITY OF RESULTS;

EID: 68849113390     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20764     Document Type: Article

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