Validation of a HeLa Mx2/Luc reporter cell line for the quantification of human type i interferons

Pharmacology

Volumn 84, Issue 3, 2009, Pages 135-144

  Seo, Young Jun ^a   Kim, Gi Hyun ^a,b   Kwak, Ho Jung ^a   Nam, Ju Sun ^a   Lee, Hwa Jeong ^b   Suh, Soo Kyung ^a   Baek, Kyung Min ^a   Sohn, Yeo Won ^a   Hong, Seung Hwa ^a  

^a Korea Food and Drug Administration   (South Korea)

^b Ewha Womans University   (South Korea)

Author keywords

HeLa cell line; Interferon ; Mx2; Reporter gene assay; Validation

Indexed keywords

3' DEAMINO 3' MORPHOLINOOXAUNOMYCIN; ALPHA2A INTERFERON; ALPHA2B INTERFERON; COMPLEMENTARY DNA; GAMMA INTERFERON; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; INTERFERON; INTERLEUKIN 1BETA; INTERLEUKIN 2; INTERLEUKIN 5; INTERLEUKIN 6; LUCIFERASE; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; CELL CULTURE; CELL DENSITY; CONTROLLED STUDY; DOSE RESPONSE; DRUG ACTIVITY; GENE EXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; MOLECULAR CLONING; PRIORITY JOURNAL; PROMOTER REGION;

ANIMALS; CATTLE; CELL LINE; CERCOPITHECUS AETHIOPS; DNA, COMPLEMENTARY; DOSE-RESPONSE RELATIONSHIP, DRUG; GENE EXPRESSION PROFILING; GENES, REPORTER; HELA CELLS; HUMANS; INTERFERON ALFA-2A; INTERFERON ALFA-2B; LUCIFERASES; LUMINESCENT MEASUREMENTS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; REPRODUCIBILITY OF RESULTS; TRANSFECTION; VERO CELLS;

EID: 68549096262     PISSN: 00317012     EISSN: None     Source Type: Journal    
DOI: 10.1159/000235158     Document Type: Article

References (26)

1. Isaacs A, Lindenmann J: Virus interference. I. The interferon. Proc R Soc Lond B Biol Sci 1957; 147: 258-267.
2. Pestka S, Langer JA, Zoon KC, Samuel CE: Interferons and their actions. Annu Rev Biochem 1987; 56: 727-777.
3. Pestka S, Krause CD, Walter MR: Interferons, interferon-like cytokines, and their receptors. Immunol Rev 2004; 202: 8-32.
4. LaFleur DW, Nardelli B, Tsareva T, Mather D, Feng P, Semenuk M, Taylor K, Buergin M, Chinchilla D, Roshke V, Chen G, Ruben SM, Pitha PM, Coleman TA, Moore PA: Interferon- kappa, a novel type i interferon expressed in human keratinocytes. J Biol Chem 2001; 276: 39765-39771.
5. Oritani K, Medina KL, Tomiyama Y, Ishikawa J, Okajima Y, Ogawa M, Yokota T, Aoyama K, Takahashi I, Kincade PW, Matsuzawa Y: Limitin: an interferon-like cytokine that preferentially influences b-lymphocyte precursors. Nat Med 2000; 6: 659-666.
6. Roberts RM, Ealy AD, Alexenko AP, Han CS, Ezashi T: Trophoblast interferons. Placenta 1999; 20: 259-264.
7. Pestka S: The interferons: 50 years after their discovery, there is much more to learn. J Biol Chem 2007; 282: 20047-20051.
8. Pestka S: The human interferon alpha species and receptors. Biopolymers 2000; 55: 254-287.
9. Hilkens CM, Schlaak JF, Kerr IM: Differential responses to IFN-alpha subtypes in human T cells and dendritic cells. J Immunol 2003; 171: 5255-5263.
10. Ortaldo JR, Herberman RB, Harvey C, Osheroff P, Pan YC, Kelder B, Pestka S: A species of human alpha interferon that lacks the ability to boost human natural killer activity. Proc Natl Acad Sci USA 1984; 81: 4926-4929.
11. Meager A: Biological assays for interferons. J Immunol Methods 2002; 261: 21-36.
12. Meager A: Assays for antiviral activity. Methods Mol Biol 2004; 249: 121-134.
13. Lleonart R, Näf D, Browning H, Weissmann C: A novel, quantitative bioassay for type I interferon using a recombinant indicator cell line. Biotechnology (NY) 1990; 8: 1263-1267.
14. Canosi U, Mascia M, Gazza L, Serlupi-Crescenzi O, Donini S, Antonetti F, Galli G: A highly precise reporter gene bioassay for type I interferon. J Immunol Methods 1996; 199: 69-76.
15. Hammerling U, Bongcam-Rudloff E, Setterblad N, Kroon R, Rehnstrom AK, Viitanen E, Andersson G, Sjodin L: The beta-gal interferon assay: a new, precise and sensitive method. J Interferon Cytokine Res 1998; 18: 451-460.
16. Bollati-Fogolin M, Muller W: Virus free, cell-based assay for the quantification of murine type I interferons. J Immunol Methods 2005; 306: 169-175.
17. Smilovic V, Caserman S, Fonda I, Gaberc- Porekar V, Menart V: A novel reporter gene assay for interferons based on cho-k1 cells. J Immunol Methods 2008; 333: 192-196.
18. Taylor MW, Grosse WM, Schaley JE, Sanda C, Wu X, Chien SC, Smith F, Wu TG, Stephens M, Ferris MW, McClintick JN, Jerome RE, Edenberg HJ: Global effect of PEG-IFNalpha and ribavirin on gene expression in PBMC in vitro. J Interferon Cytokine Res 2004; 24: 107-118.
19. McClintick JN, Jerome RE, Nicholson CR, Crabb DW, Edenberg HJ: Reproducibility of oligonucleotide arrays using small samples. BMC Genomics 2003; 4: 4.
20. (COE) CoEE, (EDQM) EDftQoM: 5.3. Statistical Analysis of Results of Biological Assays and Tests. European Pharmacopoeia, ed 6. 2007.
21. Son YW, Shin W, Jung JY, Oh IW, Jin JH, Park SY, Park TS: Study on recombinant interferon- alpha standardization. Annu Rep KFDA 2000; 4: 598-606.
22. Pfeffer LM, Dinarello CA, Herberman RB, Williams BR, Borden EC, Bordens R, Walter MR, Nagabhushan TL, Trotta PP, Pestka S: Biological properties of recombinant alphainterferons: 40th anniversary of the discovery of interferons. Cancer Res 1998; 58: 2489-2499.
23. Haller O, Stertz S, Kochs G: The Mx GTPase family of interferon-induced antiviral proteins. Microbes Infect 2007; 9: 1636-1643. (Pubitemid 350217083)
24. Platanias LC: Mechanisms of type-i- and type-ii-interferon-mediated signalling. Nat Rev Immunol 2005; 5: 375-386.
25. Sanda C, Weitzel P, Tsukahara T, Schaley J, Edenberg HJ, Stephens MA, McClintick JN, Blatt LM, Li L, Brodsky L, Taylor MW: Differential gene induction by type I and type II interferons and their combination. J Interferon Cytokine Res 2006; 26: 462-472.
26. Oritani K, Kincade PW, Tomiyama Y: Limitin: an interferon-like cytokine without myeloerythroid suppressive properties. J Mol Med 2001; 79: 168-174.