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In a real microscopy experiments there is, still, a coverslip between the immersion liquid and the sample medium. Such slab may induce a constant amount of SA that does not depend on the focusing depth. This SA can be compensated statically either with the correction collar, or with a proper index-matching immersion medium. Therefore it is not necessary to consider it in the calculations
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In a real microscopy experiments there is, still, a coverslip between the immersion liquid and the sample medium. Such slab may induce a constant amount of SA that does not depend on the focusing depth. This SA can be compensated statically either with the correction collar, or with a proper index-matching immersion medium. Therefore it is not necessary to consider it in the calculations.
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