The fast-halo assay for the assessment of DNA damage at the single-cell level

Methods in Molecular Biology

Volumn 521, Issue , 2009, Pages 517-533

  Sestili, Piero ^a  

^a UNIVERSITY OF URBINO   (Italy)

Author keywords

Apoptosis; DNA damage; DNA repair; Double strand breaks; Fluorescence microscopy; Genetic toxicology; Single strand breaks

Indexed keywords

MAMMALIA;

ETHIDIUM; FLUORESCENT DYE; HYDROGEN PEROXIDE; MUTAGENIC AGENT; SEPHAROSE; SOLVENT;

APOPTOSIS; ARTICLE; CELL SEPARATION; CELL STRAIN U937; DNA DAMAGE; DNA STRAND BREAKAGE; FLUORESCENCE MICROSCOPY; HUMAN; METHODOLOGY; STAINING;

APOPTOSIS; CELL SEPARATION; DNA BREAKS; DNA DAMAGE; ETHIDIUM; FLUORESCENT DYES; HUMANS; HYDROGEN PEROXIDE; MICROSCOPY, FLUORESCENCE; MUTAGENS; SEPHAROSE; SOLVENTS; STAINING AND LABELING; U937 CELLS;

EID: 68349140143     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-60327-815-7_30     Document Type: Article

References (33)

1. Moustacchi, E. (2000) DNA damage and repair: consequences on dose-responses, Mutat. Res. 464, 35-40 (Pubitemid 30035288)
2. Furihata, C., and Matsushima, T. (1987) Use of in vivo/in vitro unscheduled DNA synthesis for identification of organ-specific carcinogens, Crit. Rev. Toxicol. 17, 245-277 (Pubitemid 17060267)
3. Erixon, K., and Ahnstrom, G. (1979) Single-strand breaks in DNA during repair of UV-induced damage in normal human and xeroderma pigmentosum cells as determined by alkaline DNA unwinding and hydroxylapatite chromatography: effects of hydroxyurea, 5-fluoro-deoxyuridine and 1-beta-d-arabinofuranosylcy-tosine on the kinetics of repair, Mutat. Res. 59, 257-271 (Pubitemid 9093437)
4. Kohn, K.W., and Grimek-Ewig, R.A. (1973) Alkaline elution analysis, a new approach to the study of DNA single-strand interruptions in cells, Cancer Res. 33, 1849-1853
5. Ostling, O., and Johanson, K.J. (1984) Microelectrophoretic study of radiation-induced DNA damages in individual mammalian cells, Biochem. Biophys. Res. Comm. 123, 291-298 (Pubitemid 14030137)
6. Singh, N.P., McCoy, M.T., Tice, R.R., and Schneider, E.L. (1988) A simple technique for quantitation of low levels of DNA damage in individual cells, Exp. Cell. Res. 175, 184-191
7. Tice, R.R., Andrews, P. W., and Singh, N.P. (1990) The single cell gel assay: a sensitive technique for evaluating intercellular differences in DNA damage and repair, Basic Life Sci. 53, 291-301
8. Sestili, P., and Cantoni, O. (1999) Osmotically driven radial diffusion of single-stranded DNA fragments on an agarose bed as a convenient measure of DNA strand scission, Free Radic. Biol. Med. 26, 1019-1026 (Pubitemid 29198375)
9. Sestili, P., Tommasini, I., and Cantoni, O. (2001) Peroxynitrite promotes mitochon-drial permeability transition-dependent rapid U937 cell necrosis: survivors proliferate with kinetics superimposable on those of untreated cells, Free Radic. Res. 34, 513-527 (Pubitemid 32492690)
10. Sestili, P., Guidarelli, A., Dacha, M., and Cantoni, O. (1998) Quercetin prevents DNA single strand breakage and cytotoxicity caused by tert-butylhydroperoxide: free radical scavenging versus iron chelating mechanism, Free Radic. Biol. Med. 25, 196-200 (Pubitemid 28293154)
11. Sestili, P., Diamantini, G., Bedini, A., Cerioni, L., Tommasini, I., Tarzia, G., et al. (2002) Plant-derived phenolic compounds prevent the DNA single-strand breakage and cytotoxicity induced by tert-butylhydroperoxide via an iron-chelating mechanism, Biochem. J. 364, 121-128 (Pubitemid 34538954)
12. Sestili, P., Alfieri, R., Carnicelli, D., Mar tinelli, C., Barbieri, L., Stirpe, F., et al. (2005) Shiga toxin 1 and ricin inhibit the repair of H 2 O 2-induced DNA single strand breaks in cultured mammalian cells, DNA Repair (Amst) 4, 271-277 (Pubitemid 39601415)
13. Guidarelli, A., Sestili, P., Fiorani, M., and Cantoni, O. (2000) Arachidonic acid induces calcium-dependent mitochondrial formation of species promoting strand scission of genomic DNA, Free Radic. Biol. Med. 28, 1619-1627 (Pubitemid 30628465)
14. Brigotti, M., Alfieri, R., Sestili, P., Bonelli, M., Petronini, P.G., Guidarelli, A., et al. (2002) Damage to nuclear DNA induced by Shiga toxin 1 and ricin in human endothelial cells, FASEB J. 16, 365-372 (Pubitemid 34195297)
15. Wright, W.D., Lagroye, I., Zhang, P., Malyapa, R.S., and Roti, J.L. (2001) Cyto-metric methods to analyze ionizing-radiation effects, Methods Cell. Biol. 64, 251-268
16. Singh, N.P. (2000) A simple method for accurate estimation of apoptotic cells, Exp. Cell. Res. 256, 328-337 (Pubitemid 30211196)
17. Meintieres, S., Nesslany, F., Pallardy, M., and Marzin, D. (2003) Detection of ghost cells in the standard alkaline comet assay is not a good measure of apoptosis, Environ. Mol. Mutagen. 41, 260-269 (Pubitemid 36529773)
18. Kumar, I.P., Namita, S., and Goel, H.C. (2002) Modulation of chromatin organization by RH-3, a preparation of Hippophae rhamnoides, a possible role in radioprotec-tion, Mol. Cell. Biochem. 238, 1-9 (Pubitemid 35023523)
19. Grasso, S., Scifo, C., Cardile, V., Gulino, R., and Renis, M. (2003) Adaptive responses to the stress induced by hyperthermia or hydrogen peroxide in human fibroblasts, Exp. Biol. Med. (Maywood) 228, 491-498 (Pubitemid 37203666)
20. Dwarakanath, B.S., Khaitan, D., and Ravindranath, T. (2004) 2-Deoxy-D-glucose enhances the cytotoxicity of topoisomerase inhibitors in human tumor cell lines, Cancer. Biol. Ther. 3, 864-870 (Pubitemid 41351053)
21. Bacso, Z., and Eliason, J.F. (2001) Measurement of DNA damage associated with apoptosis by laser scanning cytometry, Cytometry 45, 180-186 (Pubitemid 33064139)
22. Sestili, P., Martinelli, C., and Stocchi, V. (2006) The fast halo assay: an improved method to quantify genomic DNA strand breakage at the single-cell level, Mutat. Res. 607, 205-214 (Pubitemid 44107787)
23. Brigotti, M., Carnicelli, D., Ravanelli, E., Vara, A.G., Martinelli, C., Alfieri, R.R., et al. (2007) Molecular damage and induction of proinflammatory cytokines in human endothe-lial cells exposed to Shiga toxin 1, Shiga toxin 2, and alpha-sarcin, Infect Immun 75, 2201-2207 (Pubitemid 46744306)
24. Sestili, P., Martinelli, C., Ricci, D., Fraternale, D., Bucchini, A., Giamperi, L., et al. (2007) Cytoprotective effect of preparations from various parts of Punica granatum L. fruits in oxidatively injured mammalian cells in comparison with their antioxidant capacity in cell free systems, Pharmacol. Res. 56, 18-26 (Pubitemid 46994751)
25. Guidi, C., Potenza, L., Sestili, P., Martinelli, C., Guescini, M., Stocchi, L., et al. (2008) Differential effect of creatine on oxidatively-injured mitochondrial and nuclear DNA, Bio-chim. Biophys. Acta 1780, 16-26 (Pubitemid 350216949)
26. Morgan, A.R., Evans, D.H., Lee, J.S., and Pulleyblank, D.E. (1979) Review: ethidium fluorescence assay. Part II. Enzymatic studies and DNA-protein interactions, Nucleic Acids Res. 7, 571-594 (Pubitemid 10246794)
27. Cook, P.R., and Brazell, I.A. (1977) The superhelical density of nuclear DNA from human cells, Eur. J. Biochem. 74, 527-531 (Pubitemid 8080073)
28. McGlynn, A.P., Wasson, G., O'Connor, J., McKerr, G., McKelvey-Martin, V.J., and Downes, C.S. (1999) The bromodeoxyurid-ine comet assay: detection of maturation of recently replicated DNA in individual cells, Cancer Res. 59, 5912-5916 (Pubitemid 30004950)
29. Eriksson, S., Nygren, J., and Ahnstrom, G. (2002) Matrix association of earlyand late-replicating chromatin studied by single-cell electrophoresis, Biochim. Biophys. Acta 1590, 103-108 (Pubitemid 34626773)
30. Sestili, P., Cattabeni, F., and Cantoni, O. (1996) Direct excision of 50 kb pair DNA fragments from megabase-sized fragments produced during apoptotic cleavage of genomic DNA, FEBS Lett. 396, 337-342 (Pubitemid 26377852)
31. Lunn, G., and Sansone, E.B. (1987) Ethidium bromide: destruction and decontamination of solutions, Anal. Biochem. 162, 453-458
32. Burlinson, B. et al (2007) Fourth International Workgroup on Genotoxicity testing: results of the in vivo Comet assay workgroup, Mutat. Res. 627, 31-35 (Pubitemid 46091764)
33. Ross, G.M., McMillan, T.J., Wilcox, P., and Collins, A.R. (1995) The single cell micro-gel electrophoresis assay (comet assay): technical aspects and applications. Report on the 5th LH Gray Trust Workshop, Institute of Cancer Research, 1994, Mutat. Res. 337, 57-60