A new DNA polymerase i from Geobacillus caldoxylosilyticus TK4: Cloning, characterization, and mutational analysis of two aromatic residues

Applied Microbiology and Biotechnology

Volumn 84, Issue 1, 2009, Pages 105-117

  Sandalli, Cemal ^a   Singh, Kamalendra ^b   Modak, Mukund J ^b   Ketkar, Amit ^b   Canakci, Sabriye ^c   Demir, Ismail ^c   Belduz, Ali Osman ^c  

^a RECEP TAYYIP ERDOGAN UNIVERSITY   (Turkey)

^b RUTGERS NEW JERSEY MEDICAL SCHOOL   (United States)

^c KARADENIZ TECHNICAL UNIVERSITY   (Turkey)

Author keywords

DNA polymerase I; Geobacillus caldoxylosilyticus TK4; Mutation; Thermophilic

Indexed keywords

AROMATIC RESIDUES; BACILLUS SPECIES; DNA POLYMERASE; DNA POLYMERASE I; DOUBLE STRANDED DNA; E. COLI; ERROR RATE; EXONUCLEASE; EXONUCLEASE ACTIVITY; GEOBACILLUS CALDOXYLOSILYTICUS TK4; MUTATION; MUTATIONAL ANALYSIS; NUCLEASE ACTIVITY; PURIFIED ENZYME; REVERSE-TRANSCRIPTASE; SEQUENCE HOMOLOGY; SPECIFIC ACTIVITY; STRUCTURAL ELEMENTS; THERMOPHILIC; THERMOPHILIC BACTERIA; TRIPHOSPHATE;

AMINES; AMINO ACIDS; AROMATIC COMPOUNDS; BACTERIOLOGY; DNA; ENZYMES; ESCHERICHIA COLI; GENES; MANGANESE; MANGANESE COMPOUNDS; NUCLEIC ACIDS; ORGANIC ACIDS; POLYMERS;

CLONING;

DNA DIRECTED DNA POLYMERASE BETA; DOUBLE STRANDED DNA; EXONUCLEASE; MAGNESIUM ION; RNA DIRECTED DNA POLYMERASE;

BACTERIUM; CLONE; DNA; ENZYME ACTIVITY; GENE EXPRESSION; HOMOLOGY; MUTATION;

ARTICLE; BACILLUS; BACTERIAL GENE; BINDING AFFINITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME BINDING; ENZYME PURIFICATION; ENZYME STABILITY; ESCHERICHIA COLI; GENE OVEREXPRESSION; GEOBACILLUS; GEOBACILLUS CALDOXYLOSILYTICUS; MOLECULAR CLONING; MOLECULAR WEIGHT; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; SEQUENCE HOMOLOGY; THERMOSTABILITY;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; BACILLACEAE; BACTERIAL PROTEINS; CLONING, MOLECULAR; DNA POLYMERASE I; KINETICS; MUTATION; SUBSTRATE SPECIFICITY;

BACILLUS (BACTERIUM); BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; GEOBACILLUS CALDOXYLOSILYTICUS;

EID: 68349104325     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-1962-3     Document Type: Article

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