PCR-based detection of Phomopsis azadirachtae in die-back affected neem seeds

Archives of Phytopathology and Plant Protection

Volumn 42, Issue 7, 2009, Pages 626-632

  Girish, K ^a   Bhat, S Shankara ^a   Raveesha, K A ^a  

^a UNIVERSITY OF MYSORE   (India)

Author keywords

Die back; Neem; P. azadirachtae; PCR detection; RDNA

Indexed keywords

AZADIRACHTA INDICA; PHOMOPSIS; PHOMOPSIS AZADIRACHTAE;

EID: 68149141145     PISSN: 03235408     EISSN: 14772906     Source Type: Journal    
DOI: 10.1080/03235400701287628     Document Type: Article

References (34)

1. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ. 1997. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Res 25:3389-3402.
2. Anonymous. 1992. Neem - A tree for solving global problems. National Research Council, Washington, DC: National Academy Press.
3. Anonymous. 1985. The wealth of India - Raw materials. New Delhi: Publication and Information Directorate, CSIR.
4. Benson DA, Karsch-Mizrachi I, Lipman DJ, Ostell J, Wheeler L. 2003. GenBank. Nucleic Acids Res 31:23-27.
5. Bhat SS, Sateesh MK, Devaki NS. 1998. A new destructive disease of neem (Azadirachta indica) incited by Phomopsis azadirachtae. Curr Sci 74:17-19.
6. Borneman J, Hartin JR. 2000. PCR Primers that amplify fungal rRNA genes from environmental samples. Appl Environ Microbiol 66:4356-4360.
7. Bridge PD, Arora DK, Reddy CA, Elander RP, editors. 1998. Applications of PCR in Mycology. Wallingford, UK: CAB International.
8. Clapp JP, editor. 1996. Methods in molecular biology, species diagnostics protocols: PCR and other nucleic acid methods. Totowa, NJ: Humana Press.
9. Faggian R, Bulman SR, Lawrie AC, Porter IJ. 1999. Specific polymerase chain reaction primers for the detection of Plasmodiophora brassicae in soil and water. Phytopathology 89:329-397.
10. Fathima SK. 2004. Investigations on the biology and management of Phomopsis azadirachtae on neem [PhD thesis]. Mysore, India: University of Mysore. p 135.
11. Fathima SK, Bhat SS, Girish K. 2004. Variation in Phomopsis azadirachtae, the incitant of die-back of neem. Indian Phytopath 57:30-33.
12. Hamelin RC, Bourassa M, Rail J, Dusabenyagasani M, Jacobi V, Laflamme G. 2000. PCR detection of Gremmeniella abietina, the causal agent of Scleroderris canker in pine. Mycol Res 104:527-532.
13. Henson JM, French R. 1993. The polymerase chain reaction and plant disease diagnosis. Annu Rev Phytopath 31:81-109.
14. Kageyama K, Ohyama A, Hyakumachi M. 1997. Detection of Pythium ultimum using polymerase chain reaction with species-specific primers. Plant Dis 81:1155-1160.
15. Kaminski JE, Dernoeden PH. 2005. A PCR-Based Method for the Detection of Ophiosphaerella agrostis in Creeping Bentgrass. Plant Dis 89:980-985.
16. Kulikova T, Aldebert P, Althorpe N, Baker W, Bates K, Browne P, van den Broek A, Cochrane G, Duggan K, Eberhardt R, Farque N, Garcia-Pastor M, Harte N, Kanz C, leinonen R, Lin Q, Lombord V, Lopez R, Mancuso R, McHale M, Nardone F, Silventoinen V, Stoehr P, Stoesser G, Tuli MA, Tzouvara K, Vaughan R, Wu D, Zhu W, Apweiler R. 2004. The EMBL Nucleotide sequence database. Nucleic Acids Res 32:D27-D30.
17. Lecomte P, Jean-Pierre P, Blancard D, Bastien N, Delye C. 2000. PCR assays that identify the grapevine dieback fungus Eutypa lata. Appl Environ Microbiol 66:4475-4480.
18. McCartney HA, Foster SJ, Fraaije BA, Ward E. 2003. Molecular diagnostics for fungal plant pathogens. Pest Manag Sci 59:129-142.
19. Parry DW, Nicholson P. 1996. Development of a PCR assay to detect Fusarium poae in wheat. Plant Pathol 45:383-391.
20. Prasad MNN, Bhat SS, Raj APC, Janardhana GR. 2006. Molecular detection of Phomopsis azadirachtae, the causative agent of die-back disease of neem by polymerase chain reaction. Curr Sci 91:158-159.
21. Roxburg W. 1874. Description of Indian plants. Calcutta: Thacker, Spink & Company.
22. Rozen S, Skaletsky H. 2000. Primer3 on the WWW for general users and for biologist programmers. Methods Mol Bio 132:365-386.
23. Sateesh MK, Bhat SS. 1999. Detection of seed - borne Phomopsis azadirachtae and its transmission in Azadirachta indica (Neem). Seed Sci Technol 27:753-759.
24. Sateesh MK, Bhat SS, Devaki NS. 1997. Phomopsis azadirachtae sp. nov. from India. Mycotaxon 65:517-520.
25. Schmalenberger A, Schwieger F, Tebbe CC. 2001. Effect of primers hybridizing to different evolutionarily conserved regions of the small-subunit rRNA gene in PCR-based microbial community analyses and genetic profiling. Appl Environ Microbiol 67:3557-3563.
26. Sreenivasaprasad S, Sharada K, Brown AE, Mills PR. 1996. PCR-based detection of Colletotrichum acutatum on strawberry. Plant Pathol 45:650-655.
27. Tewari DN. 1992. Monograph of neem (Azadirachta indica A. Juss.). Dehra Dun: International Book Distributors.
28. Thompson JD, Higgins DG, Gibson TJ. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673-4680.
29. White TJ, Bruns T, Lee S, Taylor JW. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ, editors. PCR protocols: A guide to methods and applications. San Diego: Academic Press. pp 315-322.
30. Willits DA, Sherwood JE. 1999. Polymerase chain reaction detection of Ustilago hordei in leaves of susceptible and resistant barley varieties. Phytopathology 89:212-217.
31. Zhang AW, Hartman GL, Curio-Penny B, Pedersen WL, Becker KB. 1999. Molecular detection of Diaporthe phaseolorum and Phomopsis longicolla from soybean seeds. Phytopathology 89:796-804.
32. Zhang AW, Hartman GL, Riccioni L, Chen WD, Ma RZ, Pedersen WL. 1997. Using PCR to distinguish Diaporthe phaseolorum and Phomopsis longicolla from other soybean fungal pathogens and to detect them in soybean tissues. Plant Dis 81:1143-1149.
33. Zhang AW, Riccioni L, Pedersen WL, Kollipara KP, Hartman GL. 1998. Molecular identification and phylogenetic grouping of Diaporthe phaseolorum and Phomopsis longicolla isolates from soybean. Phytopathology 88: 1306-1314.
34. Zhou G, Whong WZ, Ong T, Chen B. 2000. Development of a fungus-specific PCR assay for detecting low-level fungi in an indoor environment. Mol Cell Probes 14:339-348.