Flow-through imaging cytometry for characterization of Salmonella subpopulations in alfalfa sprouts, a complex food system

Biotechnology Journal

Volumn 4, Issue 6, 2009, Pages 880-887

  Bisha, Bledar ^a   Brehm Stecher, Byron F ^a  

^a IOWA STATE UNIVERSITY   (United States)

Author keywords

Flow cytometry; Food biotechnology; Imaging; Salmonella

Indexed keywords

CELL AGGREGATES; CELL MORPHOLOGY; COMPLEX FOOD SYSTEM; COMPLEX SAMPLES; CYTOMETRIC ANALYSIS; CYTOMETRY; FLOWTHROUGH; FLUORESCENCE IN SITU HYBRIDIZATION; FOOD BIOTECHNOLOGY; IMAGE-BASED; IMAGING; LOW LEVEL; NATURALLY OCCURRING; PHYSICAL INTERACTIONS; PRESENCE/ABSENCE; SALMONELLA; SALMONELLA SPP;

BIOTECHNOLOGY; CELL MEMBRANES; DEBRIS; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; LABELS; MEATS;

FRUITS;

ALFALFA; ANALYTICAL EQUIPMENT; ARTICLE; BACTERIUM CONTAMINATION; BACTERIUM DETECTION; CELL AGGREGATION; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE IN SITU HYBRIDIZATION; FOOD BIOTECHNOLOGY; HYBRIDIZATION; MICROBIAL MORPHOLOGY; NONHUMAN; PRIORITY JOURNAL; SALMONELLA; SPROUT;

FLOW CYTOMETRY; FOOD MICROBIOLOGY; IN SITU HYBRIDIZATION, FLUORESCENCE; MEDICAGO SATIVA; MICROSCOPY, ELECTRON, SCANNING; PLANT SHOOTS; SALMONELLA TYPHIMURIUM;

MEDICAGO SATIVA; SALMONELLA;

EID: 68049090702     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.200800360     Document Type: Article

References (15)

1. Fett, W. F., Naturally occurring biofilms on alfalfa and other types of sprouts. J. Food Prot. 2000, 63, 625-632.
2. Stewart, D. S., Reineke, K. F., Ulaszek, J. M., Tortorello, M. L., Growth of Salmonella during sprouting of alfalfa seeds associated with salmonellosis outbreaks. J. Food Prot. 2001, 64, 618-622.
3. Fett, W. F., Fu, T.-J., Tortorello M. L., Seed sprouts: The state of microbiological safety, in: Mathews, K. R. (Ed.), Microbiology of Fresh Produce, ASM Press, Washington, D. C., 2006, pp. 167-219.
4. Tournas, V. H., Moulds and yeasts in fresh and minimally processed vegetables, and sprouts. Int. J. Food Microbiol. 2005, 99, 71-77.
5. Robertson, L. J., Johannessen, G. S., Gjerde, B. K., Loncarevic, S., Microbiological analysis of seed sprouts in Norway. Int. J. Food Microbiol. 2002, 75, 119-126.
6. Loui, C., Grigoryan, G., Huang, H., Riley, L. W. et al., Bacterial communities associated with retail alfalfa sprouts. J. Food Prot. 2008, 71, 200-204.
7. Sivapalasingam, S., Friedman, C. R., Cohen, L., Tauxe, R. V., Fresh produce: A growing cause of outbreaks of foodborne illness in the United States, 1973 through 1997. J. Food Prot. 2004, 67, 2342-2353.
8. Brehm-Stecher, B. F., New technologies for imaging individual microbial cells, in: Frischknecht, F., Shorte, S. L. (Eds.), Imaging Cellular and Molecular Biological Function, Springer-Verlag, Berlin, 2007, pp. 313-343.
9. George, T. C., Basiji, D. A., Hall, B. E., Lynch, D. H. et al., Distinguishing modes of cell death using the ImageStream® multispectral imaging flow cytometer. Cytometry A, 2004, 59A, 237-245.
10. Ortyn, W. E., Perry, D. J., Venkatachalam, V., Liang, L. et al., Extended depth of field imaging for high speed cell analysis. Cytometry A 2007, 71A, 215-231.
11. Nordentoft, S., Christensen, H., Wegener, H. C., Evaluation of a fluorescence-labelled oligonucleotide probe targeting 23S rRNA for in situ detection of Salmonella serovars in paraffin-embedded tissue sections and their rapid identification in bacterial smears. J. Clin. Microbiol. 1997, 35, 2642-2648.
12. Kutter, S., Hartmann, A., Schmid, M., Colonization of barley (Hordeum vulgare) with Salmonella enterica and Listeria spp. FEMS Microbiol. Ecol. 2006, 56, 262-271.
13. Lantz, A. W., Brehm-Stecher, B. F., Armstrong, D. W., Combined capillary electrophoresis and DNA-fluorescence in situ hybridization for rapid molecular identification of S. Typhimurium in mixed cultures. Electrophoresis, 2008, 29, 2477-2484.
14. Gourabathini, P., Brandl, M. T., Redding, KS. Gunderson, J. H. et al., Interactions between food-borne pathogens and protozoa isolated from lettuce and spinach. Appl. Environ. Microbiol. 2008, 74, 2518-2525.
15. Vaerewijck, J. M, Sabbe, K., Baré, J., Houf, K., Microscopic and molecular studies of the diversity of free-living protozoa in meat-cutting plants. Appl. Environ. Microbiol. 2008, 74, 5741-5749.