Redesigning the reactive site loop of the wheat subtilisin/chymotrypsin inhibitor (WSCI) by site-directed mutagenesis. A protein-protein interaction study by affinity chromatography and molecular modeling

Biochimie

Volumn 91, Issue 9, 2009, Pages 1112-1122

  Bruni, Natalia ^a   Di Maro, Antimo ^b   Costantini, Susan ^c   Chambery, Angela ^b   Facchiano, Angelo M ^c   Ficca, Anna Grazia ^a   Parente, Augusto ^b   Poerio, Elia ^a  

^a UNIVERSITY OF TUSCIA   (Italy)

^b SECOND UNIVERSITY OF NAPLES   (Italy)

^c CNR   (Italy)

Author keywords

E I complexes; ESI Q TOF mass spectrometry; Time course proteolysis; WSCI mutants

Indexed keywords

ARGININE; ASPARTIC ACID; CHYMOTRYPSIN; CHYMOTRYPSIN INHIBITOR; GLYCINE; HYDROGEN; METHIONINE; PHENYLALANINE; SERINE; SUBTILISIN; THREONINE; TYROSINE; VALINE;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME DEGRADATION; ESCHERICHIA COLI; HYDROGEN BOND; MOLECULAR MODEL; NONHUMAN; PROTEIN EXPRESSION; PROTEIN HYDROLYSIS; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; SITE DIRECTED MUTAGENESIS; WHEAT; WILD TYPE;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, AFFINITY; COMPUTER SIMULATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PLANT PROTEINS; PROTEIN BINDING; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TRITICUM;

ESCHERICHIA COLI; TRITICUM AESTIVUM;

EID: 67849133188     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2009.05.010     Document Type: Article

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