ELISA protein G for the diagnosis of contagious agalactia in small ruminants

Small Ruminant Research

Volumn 84, Issue 1-3, 2009, Pages 70-75

  Campos, A C ^a   Teles, J A A ^b   Azevedo, E O ^b   Nascimento, E R ^c   Oliveira, M M M ^a   Nascimento, S A ^a   Castro, R S ^a  

^a UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO   (Brazil)

^b FEDERAL UNIVERSITY OF CAMPINA GRANDE   (Brazil)

^c FLUMINENSE FEDERAL UNIVERSITY   (Brazil)

Author keywords

Diagnostic; Epidemiology; Goat; Mycoplasma spp.; Serology

Indexed keywords

ANIMALIA; BOVIDAE; CAPRA; CAPRA HIRCUS; MYCOPLASMA; MYCOPLASMA AGALACTIAE;

EID: 67749143574     PISSN: 09214488     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.smallrumres.2009.06.006     Document Type: Article

References (30)

1. Alberti A., Robino P., Chessa B., Rosati S., Addis M.F., Mercier P., Mannelli A., Cubeddu T., Profiti M., Bandino E., Thiery R., and Pittau M. Characterisation of Mycoplasma capricolum P60 surface lipoprotein and its evaluation in a recombinant ELISA. Vet. Microbiol. 128 (2008) 81-89
2. Akerström B., Brodin T., Reis K., and Björck L. Protein G: a powerful tool for binding and detection of monoclonal and polyclonal antibodies. J. Immunol. 135 4 (1985) 2589-2592
3. Azevedo E.O., Alcântara M.D.B., Tabosa I.M., Nascimento E.R., Farias A.A., Castro R.S., and Campos C.A.M. Contagious agalactia by Mycoplasma agalactiae in dairy goats in Brazil. Epidemiologic findings. Int. Cong. Int. Organ. Mycoplasmol. (IOM) XIV (2002) Vienna, 48
4. Azevedo E.O., Alcântara M.D.B., Nascimento E.R., Tabosa I.M., Barreto M.L., Almeida J.F., Araújo M.D., Rodrigues A.R.O., Riet-Correa F., and Castro R.S. Contagious agalactia by Mycoplasma agalactiae in small ruminants in Brazil: first report. Braz. J. Microbiol. 37 (2006) 576-581
5. Buonavoglia D., Fasanella A., Greco G., and Pratelli A. A study of an experimental infection of sheep with Mycoplasma agalactiae. New Microbiol. 22 1 (1999) 27-30
6. Castro R.S., Leite R.C., Resende M., and Gouveia A.M.G. A labelled avidin-biotin ELISA to detect antibodies to caprine arthritis-encephalitis virus in goats sera. Vet. Res. Commun. 23 (1999) 512-522
7. DaMassa A.J. Recovery of Mycoplasma agalactiae from mastitic goat milk. J. Am. Vet. Med. Assoc. 183 5 (1983) 548-549
8. Dawo F., and Mohan K. Development and application of an indirect ELISA test for the detection of antibodies to Mycoplasma crocodyli infection in crocodiles (Crocodylus niloticus). Vet. Microbiol. 119 (2007) 283-289
9. De la Fe C., Assunção P., Antunes T., Rosales R.S., and Poveda J.B. Microbiological survey for Mycoplasma spp. in a contagious agalactia endemic area. Vet. J. 170 2 (2005) 257-259
10. De la Fe C., Assunção P., Rosales R.S., Antunes T., and Poveda J.B. Characterisation of protein and antigen variability among Mycoplasma mycoides subsp. mycoides (LC) and Mycoplasma agalactiae field strains by SDS-PAGE and immunoblotting. Vet. J. 171 (2006) 532-538
11. De la Fe C., Assunção P., Saavedra P., Tola S., Poveda C., and Poveda J.B. Field trial of two dual vaccines against Mycoplasma agalactiae and Mycoplasma mycoides subsp. mycoides (large colony type) in goats. Vaccine 25 12 (2007) 2340-2345
12. Egwu G.O., Ameh J.A., Aliyu M.M., and Mohammed F.D. Caprine mycoplasmal mastitis in Nigeria. Small Rumin. Res. 39 (2001) 87-91
13. Fusco M., Corona L., Onni T., Marras E., Longheu C., Idini G., and Tola S. Development of a sensitive and specific Enzyme-Linked Immunosorbent Assay based on recombinant antigens for rapid detection of antibodies against Mycoplasma agalactiae in sheep. Clin. Vaccine Immunol 14 4 (2007) 420-425
14. Gil M.C., Peña F.J., Mendoza J.H., and Gomez L. Genital lesions in an outbreak of caprine contagious agalactia caused by Mycoplasma agalactiae and Mycoplasma putrefaciens. J. Vet. Med. B. 50 10 (2003) 484-487
15. Greco G., Corrente M., Buonavoglia D., Alberti A., and Fasanella A. Inactivated vaccine induces protection against Mycoplasma agalactiae infection in sheep. New Microbiol. 25 1 (2002) 17-20
16. IBGE-Instituto Brasileiro de Geografia e Estatística, 2007. URL: http://www.ibge.gov.br/home/.
17. IBGE-Instituto Brasileiro de Geografia e Estatística, 2006. Censo Agropecuário 2006-Resultados Preliminares URL: http://www.ibge.gov.br/home/estatistica/economia/agropecuaria/censoagro/ 2006/agropecuario.pdf, 146p.
18. Kittellberger R., O'keefe J.S., Meynell R., Sewell M., Rosati S., Lambert M., Dufour P., and Pépin M. Comparison of four diagnostic tests for the identification of serum antibodies in small ruminants infected with Mycoplasma agalactiae. N. Z. Vet. J. 54 1 (2006) 10-15
19. Lambert M., Calamel M., Dufour P., Cabasse E., Vitu C., and Pépin M. Detection of false-positive sera in contagious agalactia with a multiantigen ELISA and their elimination with a protein G conjugate. J. Vet. Diagn. Invest. 10 (1998) 326-330
20. Letesson J.J., Tibor A., Van Eynde G., Wansard V., Weynants V., Denoel P., and Saman E. Humoral immune responses of Brucella-infected cattle, sheep, and goats to eight purified recombinant Brucella proteins in an indirect enzyme-linked immunosorbent assay. Clin. Diagn. Lab. Immunol 4 5 (1997) 556-564
21. Levisohn S., Davidson I., Caro Vergara M.R., and Rapoport E. Use of an ELISA for differential diagnosis of Mycoplasma agalactiae and M. mycoides subspecies mycoides (LC) in naturally infected goat herds. Res. Vet. Sci 51 1 (1991) 66-71
22. Nascimento E.R., Barreto M.L., Platenik M.O., Azevedo E.O., Tabosa I.M., Alcântara M.D.B., Almeida J.F., and Nascimento M.G.F. Contagious agalactia by Mycoplasma agalactiae in goats in Brazil. Etiologic study. Int. Cong. Int. Organ. Mycoplasmol. (IOM). XIV, Vienna (2002) 45-46
23. Oliveira, M.M.M., 2007. Diagnóstico e controle de lentivírus de pequenos ruminantes (LVPR) em caprinos (Diagnostic and control to small ruminants lentivirus (SRLV) in goats). Doctoral thesis. Universidade Federal Rural de Pernambuco, 114 pp.
24. OIE - World Organization for Animal Health, 2008a. Manual of diagnostic tests and vaccines for terrestrial animals. Principles of validation of diagnostic assays for infectious diseases. URL: http://www.oie.int/eng/normes/mmanual/2008/pdf/1.1.04_valid.pdf, pp.34-45.
25. OIE - World Organization for Animal Health, 2008b. Manual of diagnostic tests and vaccines for terrestrial animals. Contagious Agalactia. URL: http://www.oie.int/eng/normes/mmanual/2008/pdf/2.07.05_CONTAGIOUS_AGALAC TIA.pdf, pp.992-999.
26. Pépin M., Dufour P., Lambert M., Aubert M., Valognes A., Rotis T., Van de Wiele A., and Bergonier D. Comparison of three enzyme-immunosorbent assays for serologic diagnosis of contagious agalactia in sheep. J. Vet. Diagn. Invest. 15 (2003) 281-285
27. Pereira, M.G., 1999. Epidemiologia: teoria e prática (Epidemiology: theory and practice). Guanabara Koogan, Rio de Janeiro, 596 pp.
28. Sedmak J.J., and Grossberg S.E. A rapid, sensitive and versatile assay for protein using coomassie brilliant blue G250. Anal. Biochem. 79 (1977) 544-552
29. Tola S., Manunta D., Rocca S., Rocchigiani A.M., Idini G., Angioi P.P., and Leori G. Experimental vaccination against Mycoplasma agalactiae using different inactivated vaccines. Vaccine 17 22 (1999) 2764-2768
30. Woubit S., Manso-Silván L., Lorenzon S., Gaurivaud P., Poumarat F., Pellet M.-P., Singh V.P., and Thiaucourt F. A PCR for the detection of mycoplasmas belonging to the Mycoplasma mycoides cluster: Application to the diagnosis of contagious agalactia. Mol. Cell. Probes 21 (2007) 391-399